研究目的
Investigating the therapeutic effects of a specific herbal medicine on a particular disease.
研究成果
The study presents novel fluorescent pH indicators based on diazaoxatriangulenium dyes with long fluorescence decay times, high quantum yield, and excellent photostability. These indicators enable efficient elimination of autofluorescence in time-resolved measurements and are suitable for pH sensing and imaging. The planar optodes are particularly attractive for imaging pH distribution with fluorescence lifetime imaging technique, offering a versatile platform for various applications.
研究不足
The study highlights the potential of the new materials for pH sensing and imaging but notes that pH measurement in cells with nanosensors was impossible due to dye leaching in the intracellular environment. Covalent immobilization of the dyes into polymers is suggested to eliminate leaching and reduce cross-sensitivity to the nature of anions used.
1：Experimental Design and Method Selection:
The study involved the synthesis of novel fluorescent diazaoxatriangulenium (DAOTA) pH indicators and their characterization for lifetime-based self-referenced pH sensing. The dyes were decorated with phenolic receptor groups to induce fluorescence quenching via photoinduced electron transfer mechanism.
2：Sample Selection and Data Sources:
The dyes were synthesized and characterized using NMR, mass spectrometry, and photophysical measurements.
3：List of Experimental Equipment and Materials:
Equipment included a Bruker AVANCE III instrument for NMR, a Micromass TofSpec 2E Time-of-Flight Mass Spectrometer, a Varian Cary 50 UV-VIS spectrometer, and a Hitachi-F-7000 fluorescence spectrometer.
4：Experimental Procedures and Operational Workflow:
The dyes were synthesized, characterized, and immobilized into polyurethane hydrogel D4 for optode preparation. pH calibrations were performed, and fluorescence lifetime imaging (FLIM) was used to study pH distribution.
5：Data Analysis Methods:
The data were analyzed using time correlated single photon counting technique (TCSPC) and fluorescence lifetime imaging microscopy (FLIM).
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