研究目的
Developing a specific, fast response and mitochondria-targeted fluorescent probe for monitoring SO2 in mitochondria.
研究成果
The developed ratiometric fluorescent probe based on pyrido[1,2-a]benzimidazole and hemicyanine shows high sensitivity and selectivity toward SO2 in pure water, with a low detection limit of 26.7 nM. It was successfully applied for detecting endogenous SO2 in mitochondria in living Glioma cells.
研究不足
The probe's performance is limited to relatively alkaline pH conditions (5.0-10.0).
1：Experimental Design and Method Selection:
A FRET-based ratiometric fluorescent probe was designed using pyrido[1,2-a]benzimidazole as the donor and hemicyanine as the acceptor.
2：Sample Selection and Data Sources:
Glioma cells were used for imaging experiments.
3：List of Experimental Equipment and Materials:
UV-2600 spectrometer, FS-5 luminescence spectrophotometer, Varian Mercury Plus 400 spectrometer, inverted fluorescence microscope Ti 2 (Nikon).
4：Experimental Procedures and Operational Workflow:
Cells were incubated with the probe and imaged using fluorescence microscopy.
5：Data Analysis Methods:
Fluorescence intensity ratios were analyzed to determine SO2 derivatives concentrations.
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UV-2600 spectrometer
UV-2600
Shimadzu
Recording UV-vis spectra
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FS-5 luminescence spectrophotometer
FS-5
Edinburgh
Recording fluorescence spectra
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Varian Mercury Plus 400 spectrometer
Mercury Plus 400
Varian
Measuring 1H NMR and 13C NMR spectra
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inverted fluorescence microscope
Ti 2
Nikon
Cell imaging
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