摘要： Prediction of acid detergent fiber (ADF) content in corn stover depends on precise data and appropriate analytical methods. In this paper, the optimal PLSR-BPNN model was created for rapidly getting ADF content based on the optimal selection of crucial parameters and the combination of partial least squares regression (PLSR) and back propagation neural network (BPNN). Herein, Mahalanobis distance (MD) was proposed as a tool to recognize and remove outliers. Additionally, on the basis of the characteristic bands extracted by correlation coefficient method (CC), principal component analysis (PCA) was performed to select principal components (PCs) to further compress data of bands for obtaining few characteristic wavelengths. It turned out that the performance of PLSR calibration model based on the selected 10 wavelengths was best. The correlation coefficient (R2), root mean square error of prediction (RMSEP), residual predictive deviation (RPD) and relative standard deviation (RSD) of test set successively were 0.9936, 0.3765, 12.5869, and 0.0087. Besides, BPNN was proposed to cut down the nonlinear regression residual of PLSR model. Genetic algorithm (GA) was applied to avoid the problem of local minimum in network. When RMSEP decreased to the minimum value of 0.2181, PLSR-BPNN model was proven to further improve performance and reached for the best level. Finally, the result of external validation shown that the R2, RMSEP, RPD, RSD were 0.9856, 0.4590, 8.3264, 0.0110, respectively, the created model presented the best predictive performance. Hence, the proposed methods combining with NIR-spectroscopy technology can be used to determine ADF content in corn stover.