研究目的
To investigate the synthesis of tissue-engineered constructs utilizing human cadaveric donor periosteum and nanofiber PCL scaffold mats with and without added silicon in the form of SiO2 with the intent of demonstrating possible utilization of such mats to enhance or regenerate bone matrix after injury.
研究成果
The study demonstrated the successful fabrication of silica/nanoPCL scaffolds that support human periosteal cell growth in vitro and in vivo. The presence of silica in the scaffolds appeared to promote osteoblast migration and infiltration into allograft bone, suggesting potential clinical applications for bone defect repair.
研究不足
The study was limited by the variability in donor age and gender, which may affect cell behavior. Additionally, the in vivo model used was a nude mouse, which may not fully replicate human physiological conditions.
1：Experimental Design and Method Selection
The study involved the fabrication of electrospun PCL nanofiber scaffolds with different silica content (0, 0.5 and 1.0 wt%) and their use as substrates for human periosteal cell growth in vitro and in vivo. The scaffolds were characterized using SEM and ESEM, and cell viability was assessed using a PrestoBlue? viability assay.
2：Sample Selection and Data Sources
Human periosteum was retrieved from cadaveric donors within 24 hours of donor death. Periosteal cells were isolated and expanded in culture before being seeded onto the scaffolds.
3：List of Experimental Equipment and Materials
Electrospinning system, SEM (JEOL model 7401), ESEM (FEI model Quanta450 FEG), EDS (Hitachi model S-2600N SEM), PrestoBlue? cell viability assay kit, Synergy MX microplate photometer.
4：Experimental Procedures and Operational Workflow
Scaffolds were fabricated by electrospinning, characterized, and then used for in vitro cell viability studies and in vivo implantation studies in athymic mice. Histological and immunohistochemical analyses were performed on retrieved constructs.
5：Data Analysis Methods
Statistical analysis of fiber diameters and silicon content was performed using ANOVA and post-hoc Bonferroni tests. Cell viability data were analyzed using ANOVA in Minitab.
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