研究目的
To prepare DDS-modified CaO-SiO2-TiO2 hybrids by the sol-gel method and investigate the effect of Ti4+ content on physical and mechanical properties, as well as conduct in vitro and in vivo tests to evaluate bone tissue formation and material safety.
研究成果
The prepared inorganic/organic hybrids exhibit enhanced mechanical properties and bioactivity with increasing Ti content, forming apatite layers in SBF and regenerating bone tissue in vivo without causing inflammation or organ damage, making them promising for bone substitute applications.
研究不足
The study is limited to specific molar ratios and Ti concentrations; scalability and long-term effects in humans are not addressed. The in vivo tests were conducted only on rabbits over 5 months, which may not fully represent human responses.
1：Experimental Design and Method Selection:
The study used sol-gel technique to prepare CaO-SiO2-DDS and CaO-SiO2-DDS-TiO2 hybrids with varying Ti content. Methods included sol-gel synthesis, characterization via XRD, FTIR, SEM, hardness testing, in vitro SBF immersion, and in vivo implantation in rabbits.
2：Sample Selection and Data Sources:
Samples were prepared with molar ratios as per Table 1, using chemicals like calcium nitrate, TEOS, titanium n-butoxide, DDS, etc. In vivo tests used adult male rabbits.
3：List of Experimental Equipment and Materials:
Equipment included Philips X-ray diffractometer model PW 1730, SEM-JEOL JSM-T20, FTIR spectroscope Bruker Vector 22, hardness tester Omnimet automatic MHK system Model Micro Met 5114, magnetic stirrer, ICP-AES, and SEM/EDAX. Materials included calcium nitrate, TEOS, titanium n-butoxide, DDS, phosphoric acid, ethanol, distilled water, SBF, and rabbits.
4：Experimental Procedures and Operational Workflow:
Sol-gel synthesis involved mixing solutions, gelation at 37°C/7d, ageing at 60°C/7d, drying. Characterization involved XRD, FTIR, SEM, hardness measurements. In vitro tests involved SBF immersion and ion concentration analysis. In vivo tests involved surgical implantation in rabbit femur defects, followed by clinical, SEM, and biological evaluations after 5 months.
5：Data Analysis Methods:
Data analyzed using XRD for phase composition, FTIR for chemical bonds, SEM for microstructure, hardness tester for mechanical properties, ICP-AES for ion concentrations, and statistical analysis for biological data.
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