1：Experimental Design and Method Selection:

A one-pot synthesis method was used to encapsulate ICG into ZIF-8 at low temperatures to achieve high loading capacity. Methods included PXRD, TEM, UV-vis spectroscopy, FTIR, TGA, DLS, confocal microscopy, MTT assay, and in vivo studies.

2：Sample Selection and Data Sources:

ICG was purchased from Melone Pharmaceutical Co., Ltd. ZIF-8 was synthesized using Zn(NO3)2·6H2O and 2-methylimidazole. Cell lines used were PC12 and 4T1 cells. Animal models were nude mice with subcutaneous 4T1 tumors.

3：List of Experimental Equipment and Materials:

Equipment included Shimadzu XRD7000 Powder X-ray diffractometer, Hitachi S-4800 SEM, Philips CM200 TEM, Thermo Fisher Nicolet iS10 FTIR Spectrometer, Shimadzu UV-2600 UV-vis spectrometer, Netzch TG209F3 TGA, FV-1000 confocal microscope, microplate reader, infrared thermal camera. Materials included Zn(NO3)2·6H2O, 2-methylimidazole, ICG, methanol, HCl, MTT, DAPI, DMEM, FBS, P/S, Triton X-100, PBS.

4：Experimental Procedures and Operational Workflow:

Synthesis of ICG@ZIF-8 involved mixing Zn(NO3)2·6H2O and ICG in methanol, adding 2-methylimidazole solution, stirring at 0°C, centrifugation, and washing. Cellular uptake was studied by incubating cells with ICG@ZIF-8 and staining with DAPI. In vitro Zn2+ effects were assessed using MTT assay. In vivo studies involved intratumoral injection of ICG@ZIF-8 and NIR laser irradiation, with temperature monitoring and tumor volume measurement.

5：Data Analysis Methods:

Data were analyzed using PXRD for structural integrity, UV-vis for loading capacity, TGA for thermal stability, confocal microscopy for cellular uptake, MTT assay for cytotoxicity, and statistical analysis for in vivo tumor volume and weight changes.