1：Experimental Design and Method Selection:

Films were prepared by drop casting and slow drying methods. Techniques included FTIR, CD, XRD, UV–vis absorption, and fluorescence spectroscopy to analyze SF conformation and RHD aggregation.

2：Sample Selection and Data Sources:

Bombyx mori silk cocoons were used to extract silk fibroin. Films were prepared with SF:RHD molar ratios from 1:

3：005 to

4：List of Experimental Equipment and Materials:

Equipment included FTIR spectrometer (VERTEX 70v with CONFOCHECK system, Bruker), CD spectrometer (Chirascan V100, Applied Photophysics), UV–vis spectrophotometer (Shimadzu UV-2700), spectrofluorimeter (Nanolog with DeltaTime), and time-resolved fluorescence systems (FluoTime 300, PicoQuant). Materials included Na2CO3, LiBr, phosphate buffer, Rhodamine 6G (Sigma-Aldrich N°252433), and polystyrene substrates.

5：Experimental Procedures and Operational Workflow:

SF was extracted, dialyzed, and cast into films with RHD. Films were treated with methanol or water annealing as needed. Spectroscopic measurements were performed at room temperature.

6：Data Analysis Methods:

Data were analyzed using OriginLab software for spectral deconvolution, Gaussian fitting, and lifetime calculations. Exciton theory was applied for dimer geometry.