研究目的
To enhance the performance of ZnO nanorod-based enzymatic glucose sensors by introducing reduced graphene oxide (rGO) between the ZnO nanorods and ITO electrode and stimulating under UV irradiation to improve electron transfer and catalytic activity.
研究成果
The performance of ZnO nanorod-based enzymatic glucose sensors is significantly enhanced with rGO and UV irradiation, improving electron transfer, reducing recombination of electron-hole pairs, and increasing catalytic activity. Sensitivity increases from 1.47 to 3.90 μA·mM-1·cm-2, detection limit decreases from 2.3 to 0.5 μM, and sensors show high selectivity, stability, and reliability for real serum analysis, with potential applications in clinical diagnosis and other enzymatic biosensors.
研究不足
The linear ranges of the as-prepared glucose sensors are smaller than those of some pristine ZnO-based sensors, and the Michaelis-Menten constants are larger, indicating lower affinity of GOx towards glucose. Stability decreases over time due to GOx dropping off and rGO peeling. The study is limited to glucose sensing and may not generalize to other biosensors without further optimization.
1：Experimental Design and Method Selection:
The study involves synthesizing ZnO nanorods on rGO/ITO electrodes, immobilizing glucose oxidase (GOx), and conducting electrochemical tests under UV irradiation to evaluate sensor performance. Theoretical models include energy band structures and Schottky barrier effects.
2：Sample Selection and Data Sources:
ITO electrodes, rGO powder, GOx enzyme, and various chemicals were used. Samples were prepared by hydrothermal synthesis and drop-casting methods.
3：List of Experimental Equipment and Materials:
Equipment includes FE-SEM (SU8000, Hitachi), XRD (Advanced D8 A25, Panak), Raman spectrometer (HR800, Horiba JOBIN YVON), contact-angle analyzer (OCA20, Dataphysics), electrochemical workstation (CHI660D, Shanghai Chenhua Instrument Co., Ltd), and UV light source (ZTF, Xi’an TianLong Science and Technology Co., Ltd). Materials include rGO, ITO glass, GOx, Nafion, glucose, urea, uric acid, ascorbic acid, and various reagents.
4：Experimental Procedures and Operational Workflow:
Steps include preparation of rGO/ITO electrode, hydrothermal synthesis of ZnO nanorods, immobilization of GOx, coating with Nafion, and electrochemical tests (EIS, cyclic voltammetry, amperometric response) under dark and UV conditions.
5：Data Analysis Methods:
Data were analyzed using electrochemical impedance spectroscopy, cyclic voltammetry curves, calibration plots for sensitivity and detection limit, and statistical methods for selectivity and stability.
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