研究目的
Developing a rapid and accurate assay for T-2 toxin detection in food samples using an exonuclease-assisted target recycling strategy with upconversion nanoparticles and magnetic nanoparticles.
研究成果
The developed aptasensor is highly sensitive and specific for T-2 toxin detection, with a low detection limit and good recoveries in real samples, demonstrating potential for food safety applications. Future work could extend this to other toxins by substituting aptamers.
研究不足
The method may be specific to T-2 toxin and require optimization for other toxins; reliance on specific aptamers and nanoparticles could limit broad applicability; potential interference in complex food matrices not fully explored.
1：Experimental Design and Method Selection:
The study employs an exonuclease-catalyzed target recycling strategy to enhance sensitivity. UCNPs and MNPs are used as signal and capture probes, respectively, with aptamers for specific binding.
2：Sample Selection and Data Sources:
Beer samples are used as real food samples for validation, spiked with known concentrations of T-2 toxin.
3：List of Experimental Equipment and Materials:
Includes UCNPs, MNPs, T-2 toxin aptamer, cDNA, exonuclease I, chemicals from suppliers like Sigma-Aldrich and Sinopharm Chemical Reagent Co., Ltd. Equipment includes TEM, XRD, fluorescence spectrophotometer, and laser.
4：Experimental Procedures and Operational Workflow:
Synthesis and modification of nanoparticles, conjugation with DNA, hybridization, addition of exonuclease and T-2 toxin, incubation, magnetic separation, and fluorescence measurement.
5：Data Analysis Methods:
Fluorescence intensities are recorded, linear regression is used for calibration, and statistical analysis (mean ± SD) is performed with software like Origin 8.5.
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Transmission electron microscope
JEM-2100
JEOL
Characterize the morphology of synthesized nanoparticles
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Fluorescence spectrophotometer
F-7000
Hitachi Co.
Collect fluorescence spectra of samples
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X-ray diffractometer
D2 PHASER
Bruker AXS Ltd.
Characterize the crystal structure of synthesized nanoparticles
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Spectrometer
TU-1900
Purkinje General Corporation
Characterize synthesized nanoparticles
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Laser
980 nm
Beijing Hi-Tech Optoelectronic Co.
Excitation source for fluorescence measurements
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Milli-Q device
Millipore
Produce deionized water for experiments
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ELISA kit
Towei Chemical Reagent Co., Ltd.
Validate T-2 toxin detection in real samples
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