1：Experimental Design and Method Selection:

The study used a protein-directed synthesis approach with BSA as a biotemplate and stabilizer under mild conditions. Various Au/Ag molar ratios were tested to optimize nanoparticle properties.

2：Sample Selection and Data Sources:

Nanoparticles were synthesized with different Au/Ag ratios (e.g., Au-0, Au-40, Au-50, Au-60, Au-90, Au-100). Human red blood cells, A549 and MCF-7 cell lines, and zebrafish embryos were used for biocompatibility and toxicity tests.

3：0). Human red blood cells, A549 and MCF-7 cell lines, and zebrafish embryos were used for biocompatibility and toxicity tests. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Equipment included Malvern Zetasizer Nano ZS90 for zeta potential and size measurements, JEOL JEM-2100 TEM for imaging, Varian Cary-Eclipse 500 spectrophotometer for UV-vis spectra, Perkin-Elmer PHI 5000C ESCA for XPS, Olympus IX71 microscope for cell and embryo observation, and Philips Brilliance 64-slice CT system for imaging. Materials included HAuCl4, AgNO3, BSA, NaBH4, and various biochemical reagents from Sigma-Aldrich and other suppliers.

4：Experimental Procedures and Operational Workflow:

Synthesis involved mixing BSA with HAuCl4 and AgNO3, adding NaBH4, stirring, dialysis, and lyophilization. CT imaging was performed at various concentrations. Hemolytic assays, cell viability tests (CCK-8), cellular uptake studies (fluorescence and TEM), and zebrafish toxicity assessments were conducted with specific protocols.

5：Data Analysis Methods:

Data were analyzed using statistical methods for survival and hatching rates, UV-vis spectrophotometry for hemolysis, and software tools for imaging analysis.