1：Experimental Design and Method Selection:

The study designed SWNTs modified with DSPE-PEG5000-NH2 for improved water solubility and biocompatibility, conjugated with CY7 for near-infrared fluorescence imaging and enhanced photothermal conversion, and coupled with anti-IGF-1R antibody for active tumor targeting. Methods included synthesis, characterization (using TEM, AFM, Raman spectroscopy, FTIR, zeta potential, UV-VIS-NIR spectra), stability tests, cytotoxicity assays, in vitro and in vivo photothermal evaluations, flow cytometry, Western blot, immunohistochemistry, biodistribution studies using IVIS spectrum and photoacoustic imaging, and in vivo therapeutic efficacy assessments in orthotopic pancreatic cancer mouse models.

2：Sample Selection and Data Sources:

Pancreatic cancer cell lines (ASPC-1, BXPC-3, PANC-1, SW1990) and orthotopic pancreatic cancer-bearing mice were used. Data were sourced from laboratory experiments including cell culture, animal models, and various imaging and analytical techniques.

3：List of Experimental Equipment and Materials:

Equipment included atomic force microscope (AFM), Raman spectrometer, transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), zeta potential analyzer, UV-VIS-NIR spectrophotometer, small animal optical molecular imaging system (IVIS spectrum), fluorescence microscope, thermal imager, flow cytometer, Western blot apparatus, immunohistochemistry tools, photoacoustic imaging system, Cellvizio system, and confocal laser endoscope. Materials included single-walled carbon nanotubes (SWNTs), DSPE-PEG5000-NH2, CY7-NHS, anti-IGF-1R antibody, cell counting kit-8, calcein-AM, propidium iodide, reactive oxygen species assay kit, and various buffers and cell culture media.

4：Experimental Procedures and Operational Workflow:

SWNTs were carboxylated, coated with DSPE-PEG5000-NH2, conjugated with CY7 and anti-IGF-1R antibody. Characterization involved morphological, spectroscopic, and stability analyses. In vitro studies included cytotoxicity, photothermal effects, ROS production, and cellular uptake. In vivo studies involved tail vein injection of nanoprobes, biodistribution monitoring using IVIS and photoacoustic imaging, photothermal therapy under optical guidance, and assessment of therapeutic efficacy through survival and histological analysis.

5：Data Analysis Methods:

Data were analyzed using statistical methods (mean ± SD, t-tests, linear regression) with software tools for imaging and flow cytometry analysis. Quantitative measurements included fluorescence intensity, temperature changes, cell viability, and tumor-to-background ratios.