研究目的
To demonstrate a scanning radially-polarized surface plasmon microscopy (SSPM) with submicron lateral resolution and high refractive index sensitivity for verifying the quality of deposited Au film and Au structures, detecting defects on SPR chips, and imaging lipid arrays to compare thicknesses, with potential applications in measuring nanogold array properties and digital-array biosensors.
研究成果
SSPM successfully imaged various materials on gold surfaces, demonstrating its capability to identify defects in nanostructures and measure thickness changes in coating materials. It shows high resolving power in lateral and vertical directions, with potential for quality verification and monitoring in nanoscale applications, though improvements in accuracy are required.
研究不足
Fitting errors can occur when the quantity of lipid exceeds the imaging capacity of SSPM, possibly due to high refractive index and instability of lipid molecules; further calculation and fitting are needed for more accurate thickness monitoring.
1：Experimental Design and Method Selection:
The SSPM uses radially-polarized illumination to locally excite surface plasmon resonance (SPR) at a focal point on a 47 nm Au film, with Fourier plane imaging for SPR angle analysis. A LabVIEW-controlled scanning stage and RI analysis algorithm are employed to capture RI images.
2：Sample Selection and Data Sources:
Samples include polystyrene microspheres on Au film, a hexagonal gold structure array produced by microsphere lithography, and a DOPC lipid array made by dip-pen nanolithography on gold-covered coverglass.
3：List of Experimental Equipment and Materials:
Ultrafast laser (Tsunami, 780 nm), radial polarization converter (Arcoptix S.A.), objective (PlanApo 60× oil, NA=1.4, Olympus), sample stage (Proscan II, PRIOR), camera (Mightex, frame rate=10 Hz), Au film, polystyrene microspheres, DOPC lipid.
4：4, Olympus), sample stage (Proscan II, PRIOR), camera (Mightex, frame rate=10 Hz), Au film, polystyrene microspheres, DOPC lipid.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The laser beam is expanded and focused to excite SPR; raster scanning with a step size of 200 nm and rate of 100 ms/step is synchronized with camera frame rate to acquire Fourier plane images for SPR angle calculation.
5：Data Analysis Methods:
SPR coupling angle is calculated from the radius of the dark ring in Fourier images using SPR dispersion relations; effective refractive index is derived to analyze sample properties.
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