研究目的
To visualize metastasis at high resolution in the UPS PDOX model and demonstrate metastasis in a sarcoma PDOX model for the first time.
研究成果
This study successfully visualized metastasis in a sarcoma PDOX model for the first time, using RFP labeling. It provides a valuable tool for screening anti-metastatic drugs and understanding the metastatic process in sarcoma, with potential applications for patient-specific therapy.
研究不足
The study is limited to a specific sarcoma type (UPS) and may not generalize to other cancers. The use of mouse models may not fully replicate human metastasis. Small sample size (5 mice) could affect statistical power.
1：Experimental Design and Method Selection:
The study used a patient-derived orthotopic xenograft (PDOX) model with red fluorescent protein (RFP) labeling to visualize metastasis. Tumor fragments were implanted in transgenic RFP-expressing nude mice to acquire RFP-expressing stroma, then orthotopically implanted in non-transgenic nude mice. Non-invasive and ex-vivo imaging were performed using FluorVivo? and FV1000? confocal laser microscope.
2：Sample Selection and Data Sources:
A patient-derived UPS tumor was used, obtained with informed consent under an approved protocol.
3：List of Experimental Equipment and Materials:
Athymic nu/nu nude mice, transgenic RFP-expressing athymic nu/nu mice, FluorVivo? imaging system, FV1000? confocal laser microscope, optimal cutting temperature (OTC) compound, Cryomicrotome, 6-0 nylon suture.
4：Experimental Procedures and Operational Workflow:
UPS tumor fragments were implanted subcutaneously in RFP transgenic mice, then harvested and orthotopically implanted in non-transgenic mice. After six weeks, primary tumor was imaged non-invasively, and organs were resected for ex-vivo imaging and confocal microscopy.
5：Data Analysis Methods:
Presence of RFP was visualized and confirmed to indicate metastases; results were tabulated.
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