研究目的
To investigate the effects of picosecond laser micromachining on the microstructure and biofunctionalization of TC4 titanium alloy implants, optimizing laser parameters to enhance cell adhesion and proliferation.
研究成果
Picosecond laser micromachining effectively creates micro-groove structures on TC4 titanium alloy, with optimized parameters enhancing surface morphology and biofunctionalization by promoting endothelial cell adhesion and proliferation, showing potential for improved biomedical implants.
研究不足
Potential heat-affected zones and residues in laser processing; exact mechanism of contact guidance not fully understood; limited to specific alloy and cell type.
1：Experimental Design and Method Selection:
Response surface methodology was used to optimize laser parameters (laser power, scanning speed, scanning number) for creating micro-groove structures on TC4 titanium alloy.
2：Sample Selection and Data Sources:
TC4 titanium alloy plates (10 mm x 10 mm x 1.5 mm) were sanded and cleaned; elemental composition was analyzed.
3：5 mm) were sanded and cleaned; elemental composition was analyzed. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Picosecond laser device (Edgewave, PX200-3-GH), SEM (JEOL JSM-7800F Prime), 3D profilometer (KS-1100), endothelial cells (HUVECs), DMEM medium, fetal bovine serum, penicillin-streptomycin.
4：Experimental Procedures and Operational Workflow:
Laser micromachining performed with varied parameters; surface morphology analyzed by SEM and 3D profilometer; cell culture conducted for 48 hours and imaged.
5：Data Analysis Methods:
DOE statistical method using Minitab for parameter optimization; feature sizes (width, depth, spacing) measured; cell adhesion and proliferation assessed.
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