研究目的
To develop a multifunctional nanoplatform for concurrent thermo-chemotherapy of cancer cells by co-incorporating gold nanoparticles and cisplatin into alginate hydrogel, and to investigate its ultrastructural and optical characteristics.
研究成果
The ACA nanocomplex effectively combines photothermal therapy and chemotherapy, showing synergistic cytotoxicity in KB cells, mitochondrial targeting, and morphological damages. It holds promise for improving cancer treatment efficacy and reducing side effects, but further in vivo studies are needed.
研究不足
The study is in vitro, so results may not directly translate to in vivo or clinical settings. The Z-scan technique for assessing cell damage needs further validation. The specificity and long-term effects of the nanocomplex were not fully explored.
1：Experimental Design and Method Selection:
The study involved synthesizing a nanocomplex (ACA) by co-loading gold nanoparticles (AuNPs) and cisplatin into alginate hydrogel. Methods included cytotoxicity assays, photothermal performance tests, TEM for ultrastructural analysis, and Z-scan for optical nonlinear refractive index measurement.
2：Sample Selection and Data Sources:
KB human nasopharyngeal cancer cells were used, cultured in RPMI 1640 medium with FBS and antibiotics. Materials included hydrogen tetrachloroaurate, alginic acid sodium salt, cisplatin, and cell culture reagents.
3：List of Experimental Equipment and Materials:
Equipment included TEM (LEO 906; Zeiss), DLS (Malvern Zetasizer Nano ZS-90), UV-visible spectrophotometer (Rayleigh UV-1601), laser source (532 nm, Changchun New Industries Optoelectronics Tech), infrared thermal imaging camera (Testo 875–1i), and Nd:YAG laser for Z-scan. Materials included FBS (Gibco), RPMI 1640 medium, penicillin-streptomycin, trypsin-EDTA (Sigma-Aldrich), hydrogen tetrachloroaurate, alginic acid sodium salt (Sigma-Aldrich), and cisplatin.
4：Experimental Procedures and Operational Workflow:
Synthesis of ACA involved reducing HAuCl4 with sodium borohydride in alginate solution, then adding cisplatin. Cytotoxicity was assessed using MTT assay after treatments with various concentrations and laser irradiation. Photothermal performance was tested by irradiating samples and monitoring temperature. TEM samples were prepared by fixing cells in glutaraldehyde, post-fixing in osmium tetroxide, dehydrating, embedding in resin, and staining. Z-scan was performed using a 532 nm laser to measure nonlinear refractive index.
5：Data Analysis Methods:
Statistical analysis used one-way ANOVA and Tukey test with SPSS software (version 11). Data are mean ± SD, with P < 0.05 considered significant.
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Transmission Electron Microscope
LEO 906
Zeiss
To obtain information about the size and morphology of the synthesized nanocomplex.
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Dynamic Light Scattering Instrument
Zetasizer Nano ZS-90
Malvern
To measure the hydrodynamic diameter of the sample.
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Laser Source
532 nm continuous-wave
Changchun New Industries Optoelectronics Tech
For photothermal irradiation of samples.
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Infrared Thermal Imaging Camera
Testo 875–1i
Testo
To monitor temperature variation in real-time during laser irradiation.
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UV-visible Spectrophotometer
UV-1601
Rayleigh
To record the optical properties of ACA nanocomplex.
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Nd:YAG Laser
532 nm
Used as the light source for Z-scan technique.
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SPSS Software
version 11
SPSS
For statistical analysis using one-way ANOVA and Tukey test.
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