摘要： Surface-enhanced Raman scattering (SERS) significantly increases Raman scattering intensity. SERS usually uses rough-surface nano-metal materials such as gold, silver and copper as a substrate [1]. It has been widely used in surface science, analytical science and other fields [2-4]. According to the preparation method, SERS substrates can be divided into three categories: (1) solid phase substrates with fixed metal nanoparticles [5, 6]; (2) metal nanostructures prepared by nanoimprinting [7, 8]; (3) metal nanostructures prepared by template method [9-11]. Conventional SERS substrates, such as roughened surfaces of noble metals, are difficult to control and the SERS effect is unstable due to the random distribution of nanostructures. The microstructure of the nanometer regular pattern, which is processed by etching and electron beam deposition, is limited by the high cost of the instrument, the complicated production process, the difficulty to make large area and the subsequent modification of the surface. The SERS substrate prepared by a template of nanospheres array has a good controllability and reproducibility. By assembling nanospheres with different sizes to prepare the templates of different thicknesses, 2D metal films with different spacing can be obtained. The preparation of SERS substrate based on nanospheres array is simple and has low cost, easy regulation and high reproducibility. Protective agent and surfactant are not required in the preparation, meanwhile, the obtained substrate is relatively pure. PhCs are periodic structures which can be generated by a controllable self-assembly method of the spherical colloidal particles [12-15]. Light propagated inside PhCs follows Bragg's law of diffraction [16-19]. The characteristics of PhCs strongly depend on the spatial structure. Because of its cost-efficiency and easy preparation, PhC has attracted significant attentions in the application of optical sensors, optical switches [20, 21] and display devices [22-26]. The regular periodical structures can also be used as template to prepare other structural functional materials [27]. A lot of researches on the preparation and application of PhCs have been carried out in our recent work [28, 29]. Based on the special capture effect of PhC optical bandgap, the plasma effect of silver nanoparticles and the enrichment of cellulose matrix on analytes in organic solvents, we designed a combination of three properties for enhancing Raman scattering. Design concept is as follows: a PhC array which bandgap matches the incident light is used as a template, filled with a cellulose matrix that is easily degraded, and modified with silver nanoparticles. Based on the above design, a novel surface-enhanced Raman scattering substrate made by Ag-nanoparticles modified flexible cellulose photonic crystal film was firstly prepared. We synthesized an Ag-nanoparticles modified PhCs methylcellulose film (Ag-PHC-MCF) and carboxymethyl cellulose film (Ag-PHC-CMCF) SERS flexible substrates using the PhCs as templates. The innovative combination of metal nanoparticles and the ordered PhCs cellulose film (PHCCF) effectively avoided the aggregation of metal nanoparticles, as a result, the hot spots of the metal nanoparticles were distributed around the pores to ensure the uniform adsorption of the target analytes and uniform Raman signals on a large area. Through the excellent enrichment of cellulose film with pore structures, the targets in the organic solvent were captured on the cellulose film, so the Raman detection signals of the analyte were enhanced with the contact probability between the targets and the hot spots of metal nanoparticles increasing. At the same time, the light scattered on the PHCCF was enhanced significantly because of the trapping effect of PhC. Based on the original combination of the enrichment of porous methylcellulose, the plasmon resonance of Ag and the bandgap of photonic crystal, the composite film has a significant enhancement of the Raman signals. Simultaneously, due to its good degradability, it also has outstanding advantages in environmental protection. Meanwhile, it is easy to be carried and preserved because of its flexibility and light texture, which has great superiorities in transportation and storage.Therefore, it is of great significance in industrial pollutant detection or national defense safety monitoring.