1：Experimental Design and Method Selection:

The study uses UV/Vis absorption spectroscopy, dynamic light scattering, zeta-potential measurements, and atomic force microscopy to investigate aggregate dispersions and aqueous colloids of P3HT and P3HT:PCBM. Methods include rapid injection and slow interfacial growth for aggregate preparation, and mini-emulsion for colloid formation.

2：Sample Selection and Data Sources:

P3HT with specific molecular weights and regioregularity, PCBM, solvents (chloroform, dichloromethane), and surfactant SDS are used. Samples are prepared in various CF:DCM volume ratios.

3：List of Experimental Equipment and Materials:

Equipment includes spectrophotometer (Perkin-Elmer Lambda 45), DLS instrument (Malvern Zetasizer Nano ZS), AFM (TT-AFM from AFM Workshop), syringe pump, and standard lab glassware. Materials include P3HT, PCBM, SDS, chloroform, dichloromethane, and ultra-pure water.

4：Experimental Procedures and Operational Workflow:

Aggregate dispersions are prepared by injecting DCM into P3HT/CF solutions or using interfacial growth. Colloids are formed by adding dispersions to SDS solution, sonicating, and heating to remove solvents. UV/Vis, DLS, and AFM measurements are performed on diluted samples and films.

5：Data Analysis Methods:

Data analysis involves calculating Rabs and extent of aggregation from UV/Vis spectra, particle size distributions from DLS, zeta potentials, and morphological analysis from AFM images using software like Gwyddion.