1：Experimental Design and Method Selection:

A one-pot solvothermal method was used to synthesize Fe7S8/Bi2S3 nanoflowers and Bi2S3 nanosheets for comparison. The design rationale was to combine three-dimensional morphology and composition tuning to enhance NIR absorption and photothermal performance.

2：Sample Selection and Data Sources:

Precursors including bismuth nitrate pentahydrate, iron(III) nitrate nonahydrate, and sodium diethyldithiocarbamate were used. Cell lines (HeLa and 7721) and animal models (BALB/c nude mice) were sourced from ATCC and Shanghai SLAC Laboratory Animal, LLC.

3：List of Experimental Equipment and Materials:

Instruments included transmission electron microscope (JEM-2100F), X-ray diffractometer (D/max-2550 PC), XPS spectrometer (ESCALab 250Xi), UV-Vis-NIR spectrophotometer (UV-1902PC), infrared thermal imaging camera (FLIR A300), NIR laser (808 nm, 1 W/cm2), microplate reader, ICP-AES, and others. Materials included various chemicals from Sinopharm Chemical Reagent Co., Ltd.

4：Experimental Procedures and Operational Workflow:

Synthesis involved dissolving precursors, stirring, autoclaving at 170°C for 12 h, centrifugation, and washing. Characterization included TEM, XRD, XPS, UV-Vis-NIR, photothermal effect measurement, drug loading and release assays, cytotoxicity tests, and in vivo therapy experiments.

5：Data Analysis Methods:

Statistical analysis used one-way ANOVA with P < 0.05 as significant. Photothermal conversion efficiency was calculated using Roper's method. Data were analyzed with software tools not specified.