研究目的
To develop and demonstrate a real-time label-free DNA biosensor based on a thin-core fiber interferometer for detecting DNA hybridization.
研究成果
The TCF-based biosensor successfully detects DNA hybridization through wavelength blue-shifts in transmission spectra, offering advantages of low cost, simplicity, and rapid response, with potential applications in medical diagnosis, cancer detection, and environmental engineering.
研究不足
The sensor's performance may depend on the precision of TCF corrosion and surface modification; potential for optimization in sensitivity and specificity for different DNA sequences; reliance on external equipment like OSA and SBS.
1：Experimental Design and Method Selection:
The biosensor uses a thin-core fiber (TCF) interferometer spliced between single-mode fibers (SMFs) and integrated into a microfluidic channel. Modal interference is exploited for sensing, with theoretical analysis based on phase difference equations.
2：Sample Selection and Data Sources:
Biomaterials include poly-l-lysine (PLL), PBS buffer, TE buffer, probe DNA, and target DNA, with specific sequences provided.
3：List of Experimental Equipment and Materials:
TCF (Nufern, 460-HP), SMFs (SMF-28e), fusion splicer (FITEL, ver.2), hydrofluoric acid (HF) for corrosion, micro-flow channel with tube and T-branch pipe, syringe pump, supercontinuum broadband source (SBS), optical spectrum analyzer (OSA, Yokogawa AQ6370C).
4：Experimental Procedures and Operational Workflow:
The TCF is spliced, corroded with HF to reduce diameter, inserted into a microfluidic channel. Steps involve surface functionalization with PLL, immobilization of probe DNA, injection of target DNA, and real-time monitoring of transmission spectra using OSA.
5：Data Analysis Methods:
Transmission spectra are analyzed for wavelength shifts and intensity changes; simulations using beam propagation method to analyze light characteristics in TCF with different cladding diameters.
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fusion splicer
ver.2
FITEL
Used to splice the TCF between SMFs to construct the sensor.
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optical spectrum analyzer
AQ6370C
Yokogawa
Used to monitor and measure the transmission spectra of the biosensor.
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thin-core fiber
460-HP
Nufern
Used as the core sensing element in the interferometer to enable modal interference for DNA detection.
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single mode fiber
SMF-28e
Used to splice with the TCF for light input and output in the sensor setup.
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supercontinuum broadband source
Used as the light source for the interferometer to provide broadband illumination.
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syringe pump
Used to inject liquid samples into the microfluidic channel.
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