研究目的
To develop an optical test to unveil twisting of birefringent crystals in spherulites by analyzing their microscopic appearance and birefringence patterns.
研究成果
Twisting of elongated birefringent crystals reduces birefringence uniformly and does not cause periodic banding; observed banding in spherulites is likely due to rhythmic crystallization or thickness oscillations, not helical deformations. The optical test helps discriminate between causes of banding.
研究不足
The analysis is simplified for cylindrical crystals; more complex shapes require advanced mathematical methods. The test is qualitative and may not resolve very small pitches below microscope resolution. Banding can have multiple causes, not solely twisting.
1:Experimental Design and Method Selection:
The study uses polarizing microscopy to observe spherulites formed from crystallized substances, focusing on birefringence patterns to detect helical deformations. Theoretical models of torsion and birefringence are applied.
2:Sample Selection and Data Sources:
Bacterial poly(3-hydroxybutyrate) (PHB) specimens were prepared by melting and cooling between glass coverslips to form spherulites of specific thickness (30–50 μm).
3:List of Experimental Equipment and Materials:
PHB polymer, glass coverslips, hot plate, microscope hot stage, polarizing microscope with λ/4 and λ plates, video camera, and software for image analysis.
4:Experimental Procedures and Operational Workflow:
PHB was melted at 200°C, cooled to 30°C at a controlled rate to induce crystallization, and observed under polarized light with retardation plates to analyze birefringence and color patterns.
5:Data Analysis Methods:
Images were captured and analyzed using software to interpret birefringence signs and patterns, comparing observed results with theoretical predictions.
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camera
EOS 1300D
Canon
Takes pictures of the specimens.
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polarizing microscope
Axioscope
Zeiss
Used to observe birefringence patterns in spherulites under polarized light.
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hot stage
THMS 600
Linkam
Used to control temperature during sample crystallization.
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temperature programmer
TMS91
Linkam
Programs temperature changes for the hot stage.
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video camera
TK-1085
JVC
Captures images from the microscope for analysis.
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software
Image Pro Plus 3.0
Used for image analysis and processing.
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objective
105 mm F2.8 EX DG Macro OS
Sigma
Macro lens for close-up photography.
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retardation plate
λ/4 plate
Leitz
Converts linear polarized light to circularly polarized light for birefringence analysis.
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retardation plate
λ plate
Leitz
Used to determine birefringence sign of spherulites.
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hot plate
TechoKartell TK22
TechoKartell
Used for initial melting of samples.
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glass coverslips
Linkam 3930
Linkam
Used to hold samples during preparation.
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