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<sup>99m</sup> Tc-Labeled LyP-1 for SPECT Imaging of Triple Negative Breast Cancer
摘要： Triple negative breast cancer (TNBC), the most aggressive breast cancer type, is associated with high mortality and recurrence rates. An active-targeted strategy based on homing peptides is an effective approach to diagnose and treat cancer as it can deliver imaging agents or therapeutic drugs into desired tissues and accumulate less into off-target tissues. As a homing peptide, LyP-1 has shown properties of targeting, internalization, and proapoptosis to TNBC. In the study, we designed a Technetium-99m- (99mTc-) labeled LyP-1 and investigated its feasibility for targeted single-positron emission computed tomography (SPECT) imaging of TNBC. The results showed that the LyP-1 peptide had acceptable biocompatibility in the studied concentration range and could specifically bind to TNBC cells in vitro. 99mTc-labeled LyP-1 showed high radiochemical purity and stability and could be used as a probe for targeted SPECT imaging of TNBC cells in vitro and in a TNBC tumor-bearing mouse model. Our findings indicate that this active-targeted strategy has great potential to be developed into a new imaging tool for TNBC diagnosis.
关键词： Triple negative breast cancer,LyP-1,SPECT imaging,targeted imaging,99mTc
更新于2025-09-16 10:30:52
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Effects of infrared laser moxibustion on cancer-related fatigue in breast cancer survivors
摘要： Background: Cancer-related fatigue (CRF) is the most common and distressing symptom associated with cancer treatment that breast cancer survivors (BCS) experience. We previously found that laser moxibustion may be ef?cacious for CRF. The primary aim of this study is to determine the speci?c ef?cacy of 10.6 mm infrared laser moxibustion on CRF. The secondary aim is to evaluate the effect of infrared laser moxibustion on co-existing symptoms that BCS experience. Methods: We will conduct a randomized, sham-controlled, three-arm trial of infrared laser moxibustion (ILM) against sham ILM (SILM) and waitlist control (WLC) among BCS with moderate to severe fatigue. The two intervention groups will receive either real or sham infrared laser moxibustion on four acupoints (i.e., ST36 [bilateral], CV4, and CV6) for 20 minutes each session for 6 weeks (twice per week). The primary endpoint is the change in fatigue score from Baseline to Week 6 as measured by the Chinese version of the Brief Fatigue Inventory (BFI-C). Our secondary aim is to compare the severity of co-morbidities (e.g., depression, insomnia, and pain) among the 3 groups. Discussion: The results of our trial will establish evidence for the ef?cacy of infrared laser moxibustion for CRF, a very common and challenging symptom.
关键词： breast cancer,moxibustion,infrared laser,cancer-related fatigue,cancer survivor,acupuncture
更新于2025-09-11 14:15:04
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[ACM Press the 2nd International Conference - Sydney, NSW, Australia (2018.10.06-2018.10.08)] Proceedings of the 2nd International Conference on Graphics and Signal Processing - ICGSP'18 - CNN-Based CAD for Breast Cancer Classification in Digital Breast Tomosynthesis
摘要： Digital breast tomosynthesis (DBT) is a promising new technique for breast cancer diagnosis. DBT has the potential to overcome the tissue superimposition problems that occur on traditional mammograms for tumor detection. However, DBT generates numerous three-dimensional images, thereby creating a heavy workload for radiologists. Therefore, constructing an automatic computer-aided diagnosis (CAD) system for DBT image analysis is necessary. This study compared feature-based CAD and convolutional neural network (CNN)-based CAD for breast cancer classification from DBT images. The research methods included image preprocessing, candidate tumor identification, feature generation, classification, image cropping, augmentation, CNN model design, and deep learning. The accuracy rates (standard deviation) of the CNN- and feature-based CAD for breast cancer classification were 74.85% (0.122) and 87.12% (0.035), respectively. The T value was ?6.229, and the P value was 0.00 < 0.05, which indicated that the CNN-based CAD significantly outperformed feature-based CAD. The results can be applied to clinical medicine and assist radiologists in breast cancer identification.
关键词： computer-aided diagnosis,breast cancer classification,deep learning,Digital breast tomosynthesis
更新于2025-09-11 14:15:04
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Chemo-treated 4T1 breast cancer cells radiation response measured by single and multiple cell ionization using infrared laser trap
摘要： We present a study that uses a laser trapping technique for measurement of radiation sensitivity of untreated and chemo-treated cancer cells. We used a human mammary tumor cell line (4T1) treated by an antitumor compound, 2-Dodecyl-6-methoxycyclohexa-2, 5-diene-1,4-dione (DMDD), which was extracted from the root of Averrhoa carambola L. The untreated control group, and both 2-hour and 24-hour treated groups of 4T1 cells were used in this study. The absorbed threshold ionization energy (TIE) and the threshold radiation dose (TRD) were determined using a high-power infrared laser (at 1064 nm) trap by single and multiple cells trapping and ionization. The results were analyzed using descriptive and t-statistics. The relation of the TIE and TRD to the mass of the individual cells were also analyzed for different hours of treatment in comparison with the control group. Both TIE and TRD decrease with increasing treatment periods. However, the TRD decreases with mass regardless of the treatment. Analyses of the TRD for single vs multiple cells ionizations within each group have also consistently showed this same behavior regardless of the treatment. The underlying factors for these observed relations are explained in terms of radiation, hyperthermia, and chemo effects.
关键词： threshold ionization energy,chemo-treated cancer cells,threshold radiation dose,4T1 breast cancer cells,laser trapping technique,DMDD,radiation sensitivity
更新于2025-09-11 14:15:04
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Development of a compact laser-diode based frequency domain photoacoustic sensing system: Application of human breast cancer diagnosis
摘要： We present the development of a laser diode based photoacoustic spectral response (PASR) setup capable of diagnosing human breast cancer tissues through the use of mechanobiological properties of the tissue. A detailed description of the laser driver is provided, highlighting the important characteristics of the developed driver. Furthermore, the amplifier development is described. The developed laser diode based PASR system has been characterized using standard samples. Subsequently, the developed experiment has been applied onto diagnosis of human breast tumors. Energy has been used as a parameter to differentiate between normal and malignant tissues. The results were statistically consistent and then compared with standard histopathology for correlation.
关键词： photoacoustic spectral response,breast cancer diagnosis,laser diode,mechanobiological properties
更新于2025-09-11 14:15:04
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Theranostic nanocomplex of gold-decorated upconversion nanoparticles for optical imaging and temperature-controlled photothermal therapy
摘要： The multifunctional hybrid nanomaterials could bring alternative solutions to current public health problems like cancer diagnosis and treatment. In this work, the near-infrared-activated NaYF4:Yb,Er up-conversion nanoparticles (UCNPs) were synthesized and covalently decorated with sub-10 nm gold nanoparticles (AuNPs) in a 10 min reaction. The UCNPs in the UCNPs-AuNPs complex converted the deep-penetrating 975 nm near-infrared photons into visible emissions, which were simultaneously used for multiple applications: i) plasmon-induced photothermal therapy, ii) in situ sensing and control of the temperature (nano-thermometer), and iii) contrast agent for fluorescence imaging and cell tracking at the tissues transparency window. The effective energy transfer of green emissions and consequent temperature increment was enhanced by the short separation between donor (UCNPs) and acceptor nanoparticles (AuNPs). The induced hyperthermia locally triggered irreversible cancer cell damage, considerably reducing the cell viability upon 5 min of NIR irradiation but being practically inert in the absence of infrared light exposure. In addition, the non-transferred fractions of the 525 and 545 nm green emission bands were suitable for the ratiometric temperature sensing in a physiologically relevant range (25–50 °C), which allowed to accurately monitor and control the heat generation during the photothermal therapy application (with effective temperature increase from 37 up to 45 °C). Since the 659 nm red emission was only slightly affected by the attached-gold nanospheres, the UCNPs-AuNPs nanocomplex were used to track the MCF-7 breast cancer cells. The simplicity and functionality of this nanocomplex make it an attractive alternative for simultaneous breast cancer detection and temperature-controlled photothermal therapy.
关键词： Nano-thermometer,Breast cancer,Upconversion nanoparticles,Photothermal therapy,Theranostics
更新于2025-09-11 14:15:04
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Recycling the GaN Waste from LED Industry by Pressurized Leaching Method
摘要： Background: Ductal carcinoma in situ with microinvasion (DCISM) represents ~1% of all breast cancer cases. Risk factors for lymph node (LN) metastasis and appropriate adjuvant therapy for DCISM are still widely debated. Methods: We retrieved DCISM data from the National Cancer Institute’s Surveillance, Epidemiology, and End Results registry database (1998–2013). Chi-squared tests and logistic regression models were applied to investigate the potential risks of LN metastasis. Univariate and multivariate Cox proportional hazards regressions were performed to estimate the prognostic factors of DCISM. Survival outcomes were estimated using the Kaplan–Meier method. A 1:1 propensity score matching was used to minimize potential bias. Results: Overall, 6,219 patients with DCISM met our inclusion criteria. Younger age and higher grade disease were identified as risk factors for LN metastasis. In the multivariable analysis, LN metastasis and chemotherapy were prognostic factors for worse overall survival and breast cancer-specific survival. Furthermore, propensity score matching and subgroup analysis showed that chemotherapy may not be effective for DCISM patients. Conclusion: Younger patients with high-grade disease tend to have LN involved in DCISM. Adjuvant chemotherapy might not be necessary for patients with DCISM.
关键词： ductal carcinoma in situ with microinvasion,SEER database,breast cancer,lymphatic metastasis,adjuvant chemotherapy
更新于2025-09-11 14:15:04
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The effect of vaginal erbium laser treatment on sexual function and vaginal health in women with a history of breast cancer and symptoms of the genitourinary syndrome of menopause
摘要： Objectives: To assess the effects of vaginal erbium laser treatment on the vaginal health and sexual function of postmenopausal women with a history of breast cancer. Methods: An open, prospective, therapeutic intervention study was conducted with 24 postmenopausal women with a history of breast cancer and vaginal dryness, and/or dyspareunia, who had not used vaginal hormone therapy for at least 6 months. The women were treated using a 2,940-nm Erbium: YAG laser (Etherea-MX, Athena, Sa?o Carlos, Sa?o Paulo, Brazil), with 90o and 360o scanning scopes, between August, 2017 and October, 2017 in a private clinic in a city of southeastern Brazil. Vaginal erbium laser treatment was performed at three sessions with a 30-day interval between each session. Sexual function was assessed before and 1 month after treatment using the Short Personal Experiences Questionnaire. Questions related to genitourinary symptoms were also applied. Vaginal health was assessed before each laser session using the Vaginal Health Index Score. Results: Mean age was 53.7 years. Vaginal health improved, as shown by an increased overall score (P < 0.001). The effect size was large between pretreatment and post-treatment scores for vaginal elasticity, fluid volume, epithelial integrity, and moisture. The effect size was also significant for the overall sexual function score and for the score in the dyspareunia domain between pretreatment and 1 month after the final treatment session. Conclusion: Vaginal erbium laser may represent a novel therapeutic option for improving vaginal health and sexual function in postmenopausal women with a history of breast cancer.
关键词： Sexual function,Vulvovaginal atrophy,YAG laser,Dyspareunia,Menopause,Breast cancer,Erbium
更新于2025-09-11 14:15:04
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The feasibility of dedicated breast PET for the assessment of residual tumor after neoadjuvant chemotherapy
摘要： Purpose To evaluate the utility of ring-type dedicated breast positron emission tomography (dbPET) for the detection of the residual tumor after neoadjuvant chemotherapy (NAC). Materials and methods This prospective study included 27 women with histologically proven breast cancer over a 37-month period. All patients underwent ring-type dbPET followed by whole-body PET-CT (WBPET) for preoperative tumor evaluation and re-staging after NAC. The maximum standardized uptake value (SUVmax) of the tumor lesion and the degree of confidence for the presence of the residual tumor were compared between pathological complete response (pCR) and non-pCR tumors. The sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) for the detection of a non-pCR tumor were compared between dbPET and WBPET. Results On dbPET, SUVmax was significantly higher in non-pCR than in pCR tumors (P = 0.030). The sensitivity for the detection of a non-pCR tumor was significantly higher with dbPET than with WBPET (84.2% vs 26.3%, P = 0.001). In the qualitative analysis, the sensitivity for the detection of a non-pCR tumor was also significantly higher with dbPET than with WBPET (57.9% vs 21.1%, P = 0.016). Conclusion The dbPET can provide more sensitive detection of residual tumor after NAC than can WBPET.
关键词： Breast cancer,Dedicated breast positron emission tomography,Neoadjuvant chemotherapy,Whole-body PET-CT,Residual tumor
更新于2025-09-10 09:29:36
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Applications of Molecular Spectroscopy to Current Research in the Chemical and Biological Sciences || Using Fluorescence Spectroscopy to Diagnose Breast Cancer
摘要： Optical spectroscopy methods have had considerable impact in the field of biomedical diagnostics, providing novel methods for the early or noninvasive diagnosis of various medical conditions. Among them, fluorescence spectroscopy has been the most widely explored mainly because fluorescence is highly sensitive to the biochemical makeup of tissues. It has been shown that tumors were easily detected on account of altered fluorescence properties with respect to fluorescence of ordinary tissue. Breast cancer is one of the most commonly diagnosed cancers among women in the world and also it is one of the leading causes of deaths from cancer for the female population. However, when detected in early stage, it is one of the most treatable forms of cancer. Therefore, fluorescence technologies could be highly beneficial in early detection and timely treatment of cancer. This chapter presents main results and conclusions that have been reported on the use of fluorescence spectroscopy for the investigation of breast cancer. It also gives an overview on the instruments and methodology of measurements, on the main endogenous fluorophores present in tissues, on the tissue fluorescence, and on the statistical methods that aid interpretations of fluorescence spectra. Finally, examples of using various fluorescence techniques, such as excitation, emission and synchronous spectroscopy, excitation-emission matrices, and lifetimes, for the breast cancer diagnosis are presented.
关键词： fluorescence,cancer diagnosis,breast cancer,fluorophores,tissue fluorescence
更新于2025-09-10 09:29:36