- 标题
- 摘要
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- 实验方案
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A sensitive and selective fluorescence probe for detection of hypochlorite (OCl?) and its bioimaging in live cells
摘要： A novel indolium-based fluorescent probe (probe 1) for the recognition and detection of hypochlorite (OCl?) has been explored via a double oxidation reaction mechanism. Probe 1 exhibited excellent selectivity and sensitivity for OCl? over other analytes, and with a detection limit of 0.11 μM. Meanwhile, probe 1 showed fast response towards OCl? in less than 3 min with obvious changes in color, which could be observed by naked eye. Moreover, fluorescence imaging experiments by using Eca109 cells were performed utilizing the new probe, demonstrating its practical applications in living cells.
关键词： Oxidize,Fluorescence probe,Bioimaging,Hypochlorite
更新于2025-11-21 11:24:58
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A new FRET-based ratiometric fluorescence probe for hypochlorous acid and its imaging in living cells
摘要： A novel ratiometric fluorescence probe for hypochlorous acid was constructed by coumarin and pyridinium fluorophore based on the Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. In this ICT/FRET system, the energy transfer efficiency is high to 94.3%. Moreover, the probe could respond to hypochlorous acid with high selectivity and sensitivity, and exhibited a large Stokes shift. It was interesting to find that the probe could recognize hypochlorous acid via a new mechanism, in which the a -position of carbonyl group was oxidized to form a diketone derivative. More importantly, the probe was successfully applied to the ratiometric imaging of both exogenous and endogenous hypochlorous acid in living RAW 264.7 cells, with low toxicity and high photo-stability.
关键词： New reaction mechanism,FRET,Hypochlorous acid,Fluorescence probe,Cell image,Ratiometric
更新于2025-11-21 11:08:12
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Photoluminescence enhancement <i>via</i> microwave irradiation of carbon quantum dots derived from solvothermal synthesis of <scp>l</scp> -arginine
摘要： Photoluminescence enhancement of carbon quantum dots was achieved via solvothermal synthesis followed by microwave irradiation. Nitrogen and phosphorous doped carbon quantum dots were prepared by solvothermal heating of L-arginine with phosphoric acid for 12 hours followed by microwave irradiation for 3 minutes. The photoluminescence enhancement was nearly two fold after microwave irradiation. The morphology, structure, and surface properties were the same for the solvothermal (CQDs-S) as well as after microwave (CQDs-M) irradiation. Thus, the enhancement is attributed to the decrease of surface defects within CQDs, which led to a decrease in the non-radiative transitions. The CQDs were quenched selectively by Fe3+ ions. The quenching led to the fabrication of the fluorescence probe for ferric ion determination. The CQDs-M had a low detection limit of 4.0 nM, while CQDs-S had a limit of 50 nM. This study gives a tool for enhancing photoluminescence quantum yields, which is highly desired for biosensing and bioimaging applications.
关键词： Photoluminescence enhancement,Fluorescence probe,Solvothermal synthesis,Ferric ion determination,Microwave irradiation,Carbon quantum dots
更新于2025-11-19 16:56:42
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A Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection
摘要： The accurate detection of glucose in the cellular level remains a big challenge. In this study, a signal amplification strategy mediated by silver nanocube (AgNC), glucose oxidase (GOx) and silver ion fluorescence probe (denoted as AgNC-GOx/Ag+-FP) is proposed for amplified intracellular glucose detection. The AgNC is oxidized into Ag+ by H2O2 generated from the GOx-catalyzed glucose oxidation reaction, and Ag+ remarkably enhances the red fluorescence of Ag+-FP. Our results show that the AgNC-GOx/Ag+-FP is highly sensitive and specific to glucose and H2O2. Afterwards, the feasibility of using AgNC-GOx/Ag+-FP to detect intracellular glucose is verified in five different cell lines. In summary, a sensitive and specific fluorescence amplification strategy has been developed for intracellular glucose detection.
关键词： intracellular glucose,silver nanocube,fluorescence probe,glucose oxidase,signal amplification
更新于2025-11-19 16:56:35
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Water-sensitive ratiometric fluorescent probes and application to test strip for rapid and reversible detection of water
摘要： Naphthalimide-decorated fluorinated acetamides 1 and 2 were developed as solvent-sensitive dual emissive fluorescence probes. Particularly, 1 exhibited dual emission with a large Stokes shift for water, DMF, and DMSO solvents over other various organic solvents. The dual emission might be due to the increase of intramolecular charge transfer (ICT) of the naphthalimide moiety through the association of the difluorinated acetamide group with the solvent molecules. The 1 can give rise to a ratiometric change in the dual emission and a visual fluorescent color change depending on the water contents in organic solvents, including ethanol, methanol, acetonitrile and DMF solvents. Moreover, the 1-impregnated paper strips showed a rapid and easy-to-visualize fluorescent color change enabling water detection in organic solvents. These simple-to-use paper strips were also found to be reusable over 20 times.
关键词： Naphthalimide,Ratiometric fluorescence probe,Reusable test strip,Intramolecular charge transfer (ICT),Water detection
更新于2025-11-19 16:46:39
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Benzo[ghi]perylene & Coronene as Ratiometric Reversible Optical Oxygen Nano-Sensor
摘要： The development of novel single molecular probe based optical ratiometric oxygen sensor with simple fabrication, high sensitivity, and linear response is highly desirable. Herein, benzo[ghi]perylene (BzP) and coronene (Cron) were explored as dual-emissive ratiometric oxygen nano-sensor that displayed linear plots at a low probe concentration (6 μM). The sensor fabrication was simple, and the probes exhibited efficient fluorescence quenching of monomer emission by molecular oxygen. However, the excimer emission of probe remained unaffected. Thus a ratiometric method is developed based on the dual emitting luminophore. The method can be used to estimate dissolved oxygen in liquids and 5 – 100 % of oxygen concentration in gaseous mixtures.
关键词： Coronene,Benzo[ghi]perylene,Oxygen sensing,Excimer emission,Ratiometric sensor,Fluorescence probe
更新于2025-11-14 17:04:02
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A near-infrared fluorescence probe for ultrafast and selective detection of peroxynitrite with large Stokes shift in inflamed mouse models
摘要： Peroxynitrite (ONOO?) is a kind of reactive oxygen species (ROS) which is associated with pathogenesis of many diseases. A new near-infrared fluorescence probe (DCM-OH) which based on dicyanomethylene-4H-pyrans to detect endogenous ONOO? was designed and synthesized. The two-photon absorption cross sections and large Stokes shift make the probe deeper issue penetration and lower self-absorption. The obtained results demonstrated that probe DCM-OH could sensitively detect ONOO? with a low detection limit (53 nM). What’s more, probe DCM-OH exhibited an ultrafast response rate (within 5 s) toward ONOO?, which would be in favor of tracking the highly reactive and short-lived ONOO? in the living systems. Moreover, DCM-OH was successfully employed for imaging endogenous ONOO? in HepG2/RAW 264.7 cells and further applied to visualize oxidative stress in mouse model of inflammation.
关键词： In vivo imaging,NIR,Fluorescence probe,Peroxynitrite,Large Stokes shift
更新于2025-11-14 15:29:11
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Subchronic intravenous toxicity study of biofunctional ZnO and its application as a fluorescence probe for cell-specific targeting
摘要： Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early cancer is a great challenge. Herein, we choose the visible‐light emitting zinc oxide non–core/shell type nanoparticle (NP) fluorophores (ZHIE) as prototypical materials. We have reported on these materials previously. The results showed that the ZHIE NPs exhibited good water solubility and good biocompatibility. This study was conducted to investigate the toxicity of ZHIE NPs when intravenously administered to mice repeatedly at the dose required for successful tumor imaging in vivo. Anti‐macrophage‐1 antigen (Mac1), a macrophage differentiation antigen, antibody‐conjugated ZHIE NPs successfully realized targeted imaging of murine macrophage cell line Raw264.7 cells. In conclusion, ZHIE NPs are not toxic in vivo and antibody‐conjugated ZHIE NPs have great potential in applications, such as single cell labeling.
关键词： subchronic toxicity,zinc oxide (ZnO) nanoparticles,fluorescence probe
更新于2025-09-23 15:23:52
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Highly Luminescent Nitrogen-Doped Carbon Dots as “Turn-On” Fluorescence Probe for Selective Detection of Melamine
摘要： In our work, a new and simple method for selective detection of melamine is established by developing a "turn-on" fluorescence probe based on nitrogen-doped carbon dots (N-CQDs). The N-CQDs have been facilely prepared by one-step simple hydrothermal reaction and it is highly luminescent that with 51% fluorescent quantum yield. In this sensor, the fluorescent intensity of N-CQDs was found to be efficient quenched by Fe3+, upon addition of melamine the fluorescent intensity of N-CQDs-Fe3+ could gradually recover, which may because of the competitive combination of Fe3+ and melamine leading to the departure of Fe3+ from the N-CQDs' surface. Under optimum conditions, the fluorescence intensity has a good liner relationship with melamine in the range of 2.0 to 290 μM. The probe displayed good sensitivity toward melamine with a lower detection limit of 0.67 μM. In addition, this fluorescence probe has been used for the analysis of milk samples, demonstrating the fluorescence probe has potential application in the detection of melamine.
关键词： selective detection,turn-on fluorescence probe,melamine,carbon quantum dot
更新于2025-09-23 15:23:52
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A Sensitive Near-Infrared Fluorescent Probe for Detecting Heavy Metal Ag+ in Water Samples
摘要： Silver is a common catalyst in industrial production, and the frequent use of Ag+ can cause water pollution. Thus, the detection of Ag+ in the environment is necessary to determine the level of pollution from silver. In this work, we designed a new, highly selective near-infrared (NIR) fluorescent probe QCy to detect Ag+. The probe exhibits 'turn-off' fluorescence quenching responses at 760 nm towards Ag+ over other relevant cations, with outstanding sensitivity and a low detection limit (0.03 μM), which is considerably lower than the standard of the World Health Organization (WHO) for drinking water (0.9 μM). Meanwhile, QCy showed a very good linearity at a low concentration of Ag+ with a 'naked eye' visible color change of solution from blue to red. The probe has been applied successfully for the detection of Ag+ in real water samples.
关键词： selectivity,fluorescence probe,Ag+,sensitivity,water samples
更新于2025-09-23 15:22:29