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A novel “turn-on” mitochondria-targeting near-infrared fluorescent probe for H2S detection and in living cells imaging
摘要： Hydrogen sulfide (H2S) has been considered to be involved in cytoprotective processes and redox signaling. It is very meaningful to track and analyze it in mitochondria. Herein, we report a novel “turn-on” mitochondria-targeting near-infrared fluorescent probe (Mito-NIR-SH) for detection of H2S in living cells, which was designed and synthesized by introducing 2,4-dinitrophenyl as fluorescence quenching group and H2S response moiety into Changsha near-infrared fluorophore (CS-OH). The structure of the fluorophore and the probe were characterized by 1H NMR, 13C NMR and mass spectrometry. Meanwhile, Mito-NIR-SH could quantitatively detect H2S at concentrations ranging from 0 to 30 μM with a detection limit as low as 89.3 nM, showing good chemical stability, fast “turn-on” response, selectively mitochondrial location, as well as high sensitivity and selectivity toward H2S. Based on this, it was successfully applied to imaging exogenous and endogenous H2S in living HeLa cells via confocal fluorescence microscopy.
关键词： High sensitivity and selectivity,Near-infrared fluorescent probe,Hydrogen sulfide,Mitochondria-targeting,Bioimaging
更新于2025-09-23 15:22:29
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Improved Performance of Thermally Evaporated Sb2Se3 Thin-Film Solar Cells via Substrate-Cooling-Speed Control and Hydrogen-Sulfide Treatment
摘要： Antimony selenide is a promising abundant absorber material for solar cells. However, current Sb2Se3 photovoltaic devices, which are fabricated via thermal evaporation, tend to have stoichiometric problems and show suboptimal performance. In this paper, we use a modified thermal evaporator to fabricate high-quality Sb2Se3 films. By dedicatedly cooling the substrate, we can improve both the Sb2Se3 morphology and the Sb2Se3/CdS heterojunction interface substantially. We find a suitable annealing atmosphere, H2S, which can largely compensate for possible deficiencies of Se and remove the antimony-oxide layer on the film surface. Thanks to cooling control and H2S treatment, we obtain a significantly improved efficiency (6.24%) for the Sb2Se3 solar cells. Our results indicate that this thermal evaporation technique is a promising approach to improve the large-scale fabrication of antimony chalcogenide solar cells.
关键词： Thermal evaporation,Sb2Se3,Hydrogen-sulfide,Large-scale,Post-annealing process
更新于2025-09-23 15:21:01
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Hydrogen Sulfide Gas Sensor Based on Titanium Dioxide/Amino-Functionalized Graphene Quantum Dots Coated Photonic Crystal Fiber
摘要： A novel photonic crystal fiber (PCF) Mach-Zehnder interferometer (MZI) was proposed for detecting hydrogen sulfide (H2S) gas. Two single-mode fibers (SMFs), two multi-mode fibers (MMFs), and a PCF are sequentially fused to form a MZI with SMF-MMF-PCF-MMF-SMF structure. Titanium dioxide/amino-functionalized graphene quantum dots (TiO2/af-GQDs) composite is coated on the surface of PCF as sensing membrane. The fabricated sensing membrane is characterized by X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and scanning electron microscopy (SEM). Furthermore, the properties of the sensor are examined. The results show that a uniform TiO2/af-GQDs film with a thickness of 1 μm is successfully coated on the surface of the PCF. The sensor has a sensitivity of 26.62 pm/ppm, showing a good linearity and selectivity for H2S in the range of 0~55 ppm. The response time and recovery time are about 68 s and 77 s, respectively. The sensor has the advantages of low cost, small volume and simple structure, which is suitable for on-line monitoring of H2S.
关键词： Mach-Zehnder interference,Graphene quantum dots,Titanium dioxide,Fiber-optic sensing,Hydrogen sulfide
更新于2025-09-23 15:21:01
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Design, synthesis, crystal structure and cytotoxicity studies of colorimetric fluorescent “OFF-ON” probes for rapid detection of hydrogen sulfide based on Cu(II) complex
摘要： Four colorimetric fluorescent probes based on copper(II) complexes were synthesized and the molecular structures were characterized by X-ray diffraction, ESI-HRMS and elemental analysis. When exposed to HS–, the synthesized probes showed significant color changes from yellow to red and remarkable increase of fluorescence intensity (over 80-fold). What’s more, the interaction of host-guest could be completed in 2 minutes. However, no clear color and fluorescence intensity changes were observed in the presence of other anions (AcO–, H2PO4–, F–, Cl–, Br–, I–, SO42-, SO32-, CO32-, Cys and GSH). The synthesized colorimetric fluorescent probes could detect HS– rapidly and conveniently. Cytotoxicity studies indicated that the synthesized fluorescent probes were low cytotoxicity in Hela cells and may be used to detect H2S level in vivo.
关键词： hydrogen sulfide,copper complex crystal,fluorescent probe,colorimetric detection,cytotoxicity
更新于2025-09-23 15:21:01
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Laser-Generated BiVO4 Colloidal Particles with Tailoring Size and Native Oxygen Defect for Highly Efficient Gas Sensing
摘要： To alleviate the poor sensing performance of BiVO4, developing new strategies for the fabrication of unique device with improved sensing properties is very necessary and has great practical significance. In this work, size-tailored and uniform black BiVO4 colloids with abundant oxygen vacancy were synthesized by a unique method of pulsed laser irradiation of colloidal nanoparticles (PLICN). The corresponding laser irradiation effects on the sensing properties are comparatively investigated. The results indicate that the BiVO4 nanospheres with average size of 50 nm shows best sensing properties with high sensitivity, superior selectivity, low detection limit (44 ppb) to H2S at low working temperature (75 oC). Its sensing response is over 4 times higher when comparing with that of the raw material. Further investigation manifests that laser irradiation could induce quantity of the oxygen vacancy and decrease the resistance of the sensing device, which is mainly responsible for the enhanced sensing performance. Moreover, the density functional theories (DFT) calculations suggest that the oxygen vacancies can greatly decrease the surface absorption energy with enhanced H2S absorption capability on BiVO4 surface and lower the bader charger transfer from the absorbed H2S molecules to the BiVO4, thus enabling the implementation for the enhanced gas-sensing properties.
关键词： Pulsed laser irradiation,Hydrogen sulfide,Bismuth vanadate,Nanosphere,Gas sensor
更新于2025-09-23 15:19:57
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Phosphorene as H <sub/>2</sub> S and CH <sub/>4</sub> Gas Sensor
摘要： First-principle calculations within density functional theory are carried out to investigate the adsorption of various gas molecules, including hydrogen sulfide and methane on two-dimensional monolayer phosphorene in order to fully exploit the gas sensing capabilities. The adsorption properties of different molecules in the environment on monolayer phosphorene base material is discussed in terms of the Mulliken charge transfer, energy band gap, adsorption energy. The prominent adsorption sites of methane, hydrogen sulfide, water, and oxygen on monolayer phosphorene are investigated at an atomistic level. Based on the results the transport properties of monolayer phosphorene exposed to these gas molecules are investigated both in zigzag and armchair direction using non-equilibrium Green function tuned with density functional theory. The results show that using both directions simultaneously as gas sensing can be an effective technique to distinguish hydrogen sulfide and methane gas molecules from others and demonstrate great selectivity.
关键词： phosphorene,methane,Green function,density functional theory,transport properties,device,hydrogen sulfide,gas sensors
更新于2025-09-23 15:19:57
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High performance nBn detectors based on InGaAsSb bulk materials for short wavelength infrared detection
摘要： Objective: To investigate whether hydrogen sulfide (H2S) counteracts formaldehyde (FA)-induced cognitive defects and whether the underlying mechanism is involved in the upregulation of hippocampal brain-derived neurotrophic factor (BDNF) expression. Methods: The cognitive function of rats was evaluated by the Morris water maze (MWM) test and the novel object recognition test. The content of superoxide dismutase (SOD) and malondialdehyde (MDA) in the hippocampus were detected by enzyme-linked immunosorbent assay (ELISA). The neuronal apoptosis in the hippocampal CA1 region was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end (TUNEL) staining. The expression of the BDNF protein was detected by Western blot and immunohistochemistry. Results: We found that sodium hydrosulfide (NaHS, a donor of H2S) significantly reversed the impairment in the function of learning and memory in the MWM test and the novel objective recognition task induced by intracerebroventricular injection of FA. We also showed that NaHS significantly reduced the level of MDA, elevated the level of SOD, and decreased the amount of TUNEL-positive neurons in the hippocampus of FA-exposed rats. Moreover, NaHS markedly increased the expression of hippocampal BDNF in FA-exposed rats. Conclusions: H2S attenuates FA-induced dysfunction of cognition and the underlying mechanism is involved in the reduction of hippocampal oxidative damage and apoptosis as well as upregulation of hippocampal BDNF.
关键词： Cognitive defect,Oxidative stress,Brain-derived neurotrophic factor,Formaldehyde,Hydrogen sulfide,Apoptosis
更新于2025-09-19 17:13:59
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Sweet Corn <i>(Zea mays L. var. rugosa)</i> Derived Fluorescent Carbon Quantum Dots for Selective Detection of Hydrogen Sulfide and Bioimaging Applications
摘要： We report a green, simple, and facile fluorometric method for the detection of hydrogen sulfide (S2?) using bluish green carbon quantum dots (CQDs) derived from sweet corn (Zea mays L. var. rugosa) as carbon source. The optical properties of the CQDs were studied using UV-visible, PL spectra and lifetime measurements. High-resolution transmission electron microscopy (HR-TEM), atomic force microscope (AFM) and dynamic light scattering (DLS) were used for morphological studies. X-ray diffraction (XRD) technique was used for phase analysis. Zeta potential, Fourier transform infrared (FT-IR), Raman and X-ray photoelectron spectroscopy (XPS) were used to measure the charge and composition of QDs. The prepared CQDs having size less than 2.4 nm with bluish green emission at ～ 470 nm and showed excellent stability for more than 6 months in aqueous medium. The synthesized CQDs showed highly sensitive and selective detection of sulfide ions (S2?) in aqueous medium over other toxic metal ions and inorganic salts, and the detection limit was 8 nM in the linear range from 5 to 100 nM. The synthesized CQDs had excellent hemocompatibility as evidenced from the hemolytic assay (2.86 %) and ESR estimation (7.5 mm/h) on human RBCs. The cell viability of CQDs was evaluated on Vero and A549 cell line by MTT assay, which produced more than 90 % of cell viability in both cell lines at 250 μg/mL concentration. Owing to their biocompatible nature, the synthesized CQDs were used as a fluorescent probe for in vitro bioimaging applications.
关键词： Bioimaging,Sweet corn,Sensor,Hydrogen sulfide,Carbon quantum dots
更新于2025-09-12 10:27:22
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Smartphone based dual mode in situ detection of viability of bacteria using Ag nanorods array
摘要： The in-situ and rapid detection of live and dead bacteria is essential for human and environmental care. It has become one of the biggest needs in the biological and medical sciences to prevent infectious diseases, which usually occur in hospitals and field clinics. In the current scenario, antibiotic resistance is one of the severe public health problems, which requires a quick and efficient solution. Here, we report a facile sensitive, portable, user-friendly, cost-effective and time saving approach for detection of live, dead and drug-resistant bacteria. The endogenous H2S evolution was targeted to differentiate between live and dead as well as antibiotic resistant bacteria. The silver nanorods (AgNRs) arrays sensors were fabricated by glancing angle deposition technique. The colorimetric and water wettability features of as-synthesized AgNRs are found to be highly sensitive and selective for H2S. E. coli. P. aeruginosa, B. subtilis and S. aureus were used as a model organism in this study. All the bacteria were found to produce H2S by their metabolism process. In order to detect the antibiotic resistant E. coli were grown in the presence of different concentration of ampicillin in Luria broth. A drastic visible change in color as well as wetting of AgNRs array was observed. To make the technique easy, a user-friendly and field deployable mobile app ‘Colorimetric Detector’ was developed. This technique takes only 4-6 hours whereas the conventional methods need around 24 hours for the same. This dual mode facile and, inexpensive method can be easily scaled up in the field of diagnostics.
关键词： live and dead bacteria,hydrogen sulfide,glancing angle deposition,Antibiotic resistance,silver nanorods
更新于2025-09-10 09:29:36
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Dual sites fluorescence probe for hydrogen sulfide: AIEE activity and supramolecular assembly with β-cyclodextrin
摘要： One aggregation-induced emission enhancement (AIEE) active compound based on α–cyanostilbenes and maleic anhydride group were synthesized. The “Turn-On” fluorescence recognition for H2S was evaluated both in aqueous solution and cells. It was found that cyanostilbenes group acts as the first reactive site and maleimide group acts as the second site to H2S. Supramolecular assembly with β-cyclodextrin successfully revealed the reaction mechanism of 3-state model.
关键词： α–cyanostilbenes,Hydrogen sulfide,Supramolecular assembly,Maleic anhydride,Aggregation–induced emission
更新于2025-09-09 09:28:46