- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Introducing Manganese-Doped Lead Halide Perovskite Quantum Dots: A Simple Synthesis Illustrating Optoelectronic Properties of Semiconductors
摘要： Quantum dots (QDs) are considered useful for demonstrating quantum phenomena in undergraduate laboratories due to their monodisperse size and excellent optical properties. Although doping has an increasingly important role in QD fabrication in the semiconductor field, it has rarely been discussed in the context of the undergraduate laboratory. In this work, a simple synthesis and characterization method for Mn-doped CsPbCl3 QDs for an upper-level undergraduate inorganic chemistry laboratory is reported. The Mn-doped CsPbCl3 system benefits from a simplified synthesis and straightforward characterization. This experiment introduces QD research to students and offers opportunities for instructors to discuss many important concepts in inorganic chemistry, such as energy band theory, particle-in-a-box model, electron paramagnetic resonance, ligand field theory, and nanochemistry.
关键词： Inorganic Chemistry,Crystal Field/Ligand Field Theory,EPR/ESR Spectroscopy,Upper-Division Undergraduate,Hands-On Learning/Manipulatives,Laboratory Instruction,Nanotechnology
更新于2025-11-20 15:33:11
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Simple Acid Vapor Method for Production of HCl and DCl Gas for IR Spectroscopy
摘要： We report here a method that utilizes the vapor pressure of HCl(g) above a concentrated HCl(aq) solution as an extremely simple and safe method to produce small amounts of HCl vapor in an IR gas cell. This vapor pressure approach can also be used to produce DCl(g) by introducing a few drops of concentrated DCl(aq) into the gas cell.
关键词： Physical Chemistry,Laboratory Instruction,IR Spectroscopy,Upper-Division Undergraduate,Hands-On Learning/Manipulatives
更新于2025-09-23 15:21:01
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Identification of Proteins Using Four Methods of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
摘要： An upper-division undergraduate- or graduate-level laboratory experiment, challenging students to identify bovine serum albumin, equine myoglobin, bovine calmodulin, and bovine cytochrome c, uses four methods of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): linear positive, in-source decay, peptide mass fingerprint, and postsource decay. Individual hands-on experience with each method is provided in a three week experiment. The importance of sequencing proteins/peptides when identifying proteomic targets using MALDI-TOF MS is emphasized.
关键词： Mass Spectrometry,Hands-On Learning/Manipulatives,Upper-Division Undergraduate,Laboratory Instruction,Graduate Education/Research,Bioanalytical Chemistry,Biotechnology,Analytical Chemistry,Biochemistry
更新于2025-09-19 17:13:59
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Analytical Methods for Monitoring the Degradation of Plasmonic Nanoparticles in Solution: An Instrumental Analysis Laboratory Exercise
摘要： Nanoparticles are an increasingly important in chemistry research, but currently there are few opportunities for chemistry students to work with nanoparticles in undergraduate analytical courses. In this exercise, designed for an analytical or instrumental analysis lab course, students have the opportunity to work with solutions of silver nanoparticles and analyze them with two instrumental techniques. UV?vis spectrophotometry is utilized to monitor the optical behavior of silver nanoparticles over the course of the experiment. ICP-OES analysis determines the concentration of total silver species present in solution based on storage conditions. This exercise also introduces students to the concept of localized surface plasmon resonance and its role in producing the vivid colors often associated with plasmonic nanoparticles embedded in stained glass.
关键词： Upper-Division Undergraduate,Instrumental Methods,Collaborative/Cooperative Learning,Laboratory Instruction,Analytical Chemistry,Nanotechnology,Problem Solving/Decision Making
更新于2025-09-19 17:13:59
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Detecting and Quantifying Microplastics in Bottled Water using Fluorescence Microscopy: A New Experiment for Instrumental Analysis and Environmental Chemistry Courses
摘要： Microplastics (MPs) are small plastic particles (<5 mm in size) that are ubiquitous in the environment and have even been detected in bottled water. In this laboratory experiment, suited for instrumental or environmental chemistry classes, students detect and quantify MPs in bottled water by filtering and staining them with Nile red dye prior to utilizing fluorescence microscopy. Instrumental concepts in fluorescence spectroscopy are reinforced as students build a low-cost fluorescence microscope and use it to collect images of the fluorescing MPs for counting purposes and assessing morphology. The exercise introduces students to MP pollution, an emerging field of chemical research, and motivates and engages them helping to form connections beyond the classroom. Each group detected MPs in their bottled water, and many were surprised by how many they found. Overall, the hands-on experiment received positive feedback from students, and postexperiment assessments showed marked improvement in their understanding of the principles of fluorescence and of the growing problem of MP pollution.
关键词： Analytical Chemistry,Upper-Division Undergraduate,Collaborative/Cooperative Learning,Water/Water Chemistry,Instrumental Methods,Environmental Chemistry,Hands-On Learning/Manipulatives
更新于2025-09-12 10:27:22
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Chemical cross-linking of a variety of green fluorescent proteins as F?rster resonance energy transfer donors for Yukon orange fluorescent protein: A project-based undergraduate laboratory experience
摘要： F?rster resonance energy transfer (FRET) is the basis for many techniques used in biomedical research. Due to its wide use in molecular sensing, FRET is commonly introduced in many biology, chemistry, and physics courses. While FRET is of great importance in the biophysical sciences, the complexity and dif?culty of constructing FRET experiments has resulted in limited usage in undergraduate laboratory settings. Here, we present a practical undergraduate laboratory experiment for teaching FRET using a diverse set of green-emitting ?uorescent proteins (FPs) as donors for a cross-linked Yukon orange FP. This laboratory experiment enables students to make the connection of basic lab procedures to real world applications and can be applied to molecular biology, biochemistry, physical chemistry, and biophysical laboratory courses.
关键词： Upper-division undergraduate,orange ?uorescent protein,?uorescence lifetimes,proteins,green ?uorescent protein,biochemistry,TCSPC,?uorescence spectroscopy
更新于2025-09-10 09:29:36
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Process Analytical Technology for Online Monitoring of Organic Reactions by Mass Spectrometry and UV–Vis Spectroscopy
摘要： Process analytical technologies (PATs) are used in the pharmaceutical and other industries to monitor chemical processes in real-time with analytical instrumentation. Each PAT analyzer brings its own advantages and disadvantages to the process, and multiplexing PAT analyzers can be advantageous as they can provide confirmatory measurements and increase the overall confidence of the analysis. In a pedagogical setting, online reaction monitoring enables the instructor to explain the fundamentals of instrumental techniques while highlighting pertinent real-world applications. Here we present a teaching laboratory exercise in which students monitor an amide bond formation reaction by flow UV?vis spectroscopy and by mass spectrometric analysis. The coupling of these two specific instruments allows students to investigate two instruments in one laboratory exercise, to explore the fundamentals behind each, and to contemplate the advantages and disadvantages of these instrumental methods. The coupling of the two instruments was aided by 3D printing which allowed students to explore how rapid prototyping can enable the development of innovative procedures.
关键词： Organic Chemistry,Mass Spectrometry,UV?Vis Spectroscopy,Laboratory Instruction,Instrumental Methods,Hands-On Learning/Manipulatives,Upper-Division Undergraduate,Analytical Chemistry,Reactions
更新于2025-09-04 15:30:14