- 标题
- 摘要
- 关键词
- 实验方案
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A Photostable AIEgen for Specific and Real-time Monitoring of Lysosomal Processes
摘要： Lysosomes are recognized as advanced organelles involved in many cellular processes, and are also considered as crucial regulators of cell homeostasis. The current strategies for monitoring activities of lysosomes exhibit some limitations. Herein, we synthesized a novel fluorescent probe named 2M-DPAS with AIE characteristics, which was proved to have significant advantages of good biocompatibility, high selectivity, bright emission and excellent photostability. Based on those, 2M-DPAS can be used to continuously monitor the dynamic changes of lysosomes including autophagy and mitophagy, as well as to track the process of endocytosis of macromolecules in lysosomes, which are benefit to better know about the lysosomes-related diseases.
关键词： Aggregation-induced emission,Lysosomes,Autophagy,Monitoring,Phagocytosis of macromolecules
更新于2025-09-23 15:23:52
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Nuclear Spin Singlet States in Photoactive Molecules: From Fluorescence/NMR Bimodality to a Bimolecular Spin Singlet State Switch
摘要： Nuclear spin singlet states are silent states in nuclear magnetic resonance (NMR). However, they can be probed indirectly and offer great potential for the development of contrast agents for magnetic resonance imaging (MRI). Here, we are introducing two novel concepts: Firstly, we investigate the bimodal NMR/fluorescence properties of 13C2-tetraphenylethylene. It possesses a long-lived singlet state in organic solvents, which shortens upon the addition of water. This simultaneously increases the fluorescence emission in a substantial enhancement of fluorescence. Secondly, we introduce a bimolecular singlet state switch based on 3-2H-coumarin containing an isolated proton. Upon UV-light exposure, a dimer forms leading to a coupling between two previously isolated protons. A nuclear spin singlet state can now be populated. Excitation with a wavelength of 254 nm results in partly cleavage of the molecule back into its monomer.
关键词： Photoswitches,NMR spectroscopy,Aggregation induced emission,Fluorescence,Nuclear spin singlet state
更新于2025-09-23 15:23:52
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Facile fabrication of cross-linked fluorescent organic nanoparticles with aggregation-induced emission characteristic via the thiol-ene click reaction and their potential for biological imaging
摘要： Over the past several years, the biomedical applications of fluorescent organic nanoparticles (FONs) with aggregation-induced emission (AIE) feature have been extensively explored because the AIE-active FONs could effectively overcome the aggregation caused quenching (ACQ) effect of FONs based on conventional organic dyes. The development of novel methods for synthesis of AIE-active FONs plays a centre role for their biomedical applications. In this work, we reported a facile one-step thiol-ene click reaction for fabrication of AIE-active FONs through conjugation of acrylated PEG and AIE-active tetraphenylethylene (TPE) with two ene bonds using pentaerythritol tetra(3-mercaptopropionate) as the linkage. The successful synthesis of TPE containing AIE-active copolymers was evidenced by various characterization techniques. The particle size and fluorescence properties of the resultant TPE-S-PEG copolymers were evaluated by transmission electronic microscopy and fluorescence spectroscopy. Moreover, the cell viability and cell uptake behavior was also examined to evaluate their potential for biological imaging. We demonstrated that the cross-linked TPE-S-PEG show small size, high water dispersibility, low cytotoxicity and strong fluorescence for tracing. All of these advantages endow the TPE-S-PEG FONs great potential for biological imaging applications. Furthermore, this novel click reaction can take place under mild experimental conditions with high efficiency. It could be also further expanded for preparation of multifunctional AIE-active materials due to the universality of the thiol-ene click reaction and good precursor applicapability. Taken together, we have developed a novel and effective thiol-ene click reaction to fabricate the cross-linked AIE-active FONs, which display excellent physicochemical and biological properties and are promising for biomedical applications.
关键词： thiol-ene click reaction,Aggregation-induced emission,biomedical applications,fluorescent organic nanoparticles,cross-linked FONs
更新于2025-09-23 15:23:52
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An AIEgen-based fluorescent probe for highly selective and specific imaging of lipid droplets in L02 and HepG2 cells
摘要： Lipid droplets (LDs), as dynamic organelles, are associated with physiologically and pathologically metabolic processes. Especially, in human liver cancer cells (HepG2 cells) the lipid metabolism shows significant difference by comparing with the human normal liver cell (L02 cells). In this work, a new highly selective and specific fluorescent probe TPA-SD for the detection of LDs has been rationally designed and easily prepared from triphenylamine and salicylaldehyde, which possesses aggregation-induced emission (AIE) and excited state intramolecular proton transfer characteristics (ESIPT). Ideal probes for LDs imaging require high concentration accumulation of fluorophores in LDs and lack of self-quenching in the aggregated state, so the AIE property is necessary. TPA-SD can successfully and specifically accumulate in LDs, thus facilitating clearly distinguishing L02 cells from HepG2 cells according to obvious difference in the number of dot-shaped LDs marked by TPA-SD. The dot-shaped LDs in the HepG2 cells is found to be obviously more than that in the L02 cells. Moreover, the spatial distribution of LDs could be identified by TPA-SD. The results indicate TPA-SD possesses great potential in imaging and diagnosis of LDs-related diseases.
关键词： fluorescent probe,aggregation-induced emission,dot-shaped LDs number,lipid droplets (LDs) imaging
更新于2025-09-23 15:23:52
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SwissKnife-Inspired Multifunctional Fluorescence Probes for Cellular Organelle Targeting Based on Simple AIEgens
摘要： Facile, efficient and mass production of aggregation-induced emission (AIE) luminogens (AIEgens) with excited-state intramolecular proton transfer (ESIPT) characteristics was achieved by one-step condensation reaction of 2-(hydrazonomethyl)phenol with benzaldehydes. The function of as-prepared AIEgens could be tuned easily by varying the functional group carrying on the phenyl ring of benzaldehyde just like Swiss knife handle. The suitable distance and angle of intramolecular hydrogen bond in these AIEgens endowed them with ESIPT properties, intense solid-state luminescence and large Stokes shifts (155?169 nm). These AIEgens could not only serve as biological probes showing specific targeting to lipid droplets, endoplasmic reticulum and lysosomes, respectively, but also generate reactive oxygen species upon visible light irradiation to make them promise for photodynamic therapy.
关键词： Fluorescence probes,Aggregation-induced emission,Excited-state intramolecular proton transfer,Photodynamic therapy,Organelle-targeting
更新于2025-09-23 15:23:52
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Alkyl chains length dependent fluorescence emission and reversible mechanofluorochromism of AIEE-based quinoline derivatives
摘要： Five quinoline derivatives containing different alkyl chains (QLACn, n ? 2, 4, 6, 8, 12) were synthesized to investigate the effect of alkyl chains on their photophysical properties. The ?uorescent properties of the QLACn were affected by the alkyl chains, which indicated obvious blue-shifting trend and ?uorescence emission are alkyl chains length-dependent by grinding-induced spectral shifts (DlMFC). Longer alkyl-length revealed larger DlMFC. Powder X-ray diffraction (PXRD), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC) measurements indicated that the transformation between microcrystalline and amorphous states upon external stimuli should be responsible for the observed reversible MFC behavior.
关键词： Alkyl chains length-dependent behavior,Quinoline derivatives,Aggregation-induced emission enhancement (AIEE) behavior,Mechano?uorochromism behavior
更新于2025-09-23 15:23:52
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Visualize Embryogenesis and Cell Fate Using Fluorescent Probes with Aggregation-Induced Emission
摘要： Horseradish peroxidase (HRP) and fluorogen-dextran conjugate are tracers extensively used for injection-based lineage tracing. However, HRP is sensitive to proteolytic digestion while the high-molecular-weight dextran may have antigenicity. Small molecular tracers can overcome these problems, but they usually diffuse from labeled cells, causing inaccurate information. Herein, we developed a small-molecular-weight fluorogen with aggregation-induced emission (AIEgen) for embryonic cell tracing with strong signals against tracer dilution caused by cell division. Once injected into the ancestor cells, the AIEgen can be entrapped in the cells without leakage because of the two hydrophilic and neutral arms. Consequently, it can specifically trace the progenies of the treated ancestor cells. More importantly, the operating concentration of AIEgen can be much higher than those of fluorogens with aggregation-caused quenching (ACQ), which provides bright signals in daughter cells during embryonic cell tracing, thus overcoming the problem of fast signal degrading typically encountered with the use of traditional cell tracers.
关键词： bright signals,small molecule,aggregation-induced emission,cell entrapped,embryonic cell,zebrafish lineage tracing
更新于2025-09-23 15:23:52
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Unprecedented Multicolor Photoluminescence from Hyperbranched Poly(amino ester)s
摘要： A novel kind of water-soluble fluorescent hyperbranched poly(amino ester) (PAE) is prepared through a one-pot polycondensation reaction of citric acid (CA) and N-methyldiethanolamine (NMDEA). The PAE exhibits enhanced and red-shift fluorescence with increasing solution concentration, showing distinct aggregation-induced emission character. Interestingly, the resulting PAE exhibits tunable photoluminescence from blue, cyan, and green to red irradiated by altering the excitation wavelengths. Such unique emission of non-conjugated PAE is attributed to the clustering of ester and tertiary amine groups derived from PAE self-assembly aggregates. Moreover, the fluorescence of PAE is very sensitive to Fe3+ ions. The facile preparation and unique optical features make PAE potentially useful in numerous applications such as multicolor cellular imaging, Fe3+ ions probe, and light-emitting diodes.
关键词： aggregation-induced emission,poly(amino ester)s,water-soluble polymers,hyperbranched,fluorescence
更新于2025-09-23 15:23:52
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Rational Design of Near-Infrared AIE-Active Probes: In Situ Mapping of Amyloid-β Plaques with Ultra-Sensitivity and High-Fidelity
摘要： High-fidelity mapping of amyloid-β (Aβ) plaques is critical for the early detection of Alzheimer’s disease. However, in vivo probing of Aβ plaques by commercially available Thioflavin derivatives (ThT or ThS) has proven to be extremely limited, as evident by the restriction of enrichment quenching effect, low signal-to-noise (S/N) ratio and poor blood-brain barrier (BBB) penetrability. Herein, we demonstrate a rational design strategy of near-infrared (NIR) aggregation-induced emission (AIE)-active probes for Aβ plaques, through introducing lipophilic π-conjugated thiophene-bridge for extending to NIR wavelength with enhancement of BBB penetrability, and tuning the substituted position of sulfonate group for guaranteeing specific hydrophilicity to keep fluorescence-off state before binding to Aβ deposition. Probe QM-FN-SO3 has well settled the AIE dilemma between lipophilic requirement for longer emission and aggregation behavior from water to protein fibrillogenesis, thus making a breakthrough in high-fidelity feedback on in vivo detection of Aβ plaques with remarkable binding affinity, and serving as an efficient alternative to the commercial probe ThT or ThS.
关键词： Fluorescent probe,In vivo imaging,Aggregation-induced emission,Amyloid-β plaques,Near-infrared
更新于2025-09-23 15:23:52
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Freezing Induced Turn-on Modality for Real-time Imaging in Cryosurgery
摘要： Cryosurgery has attracted great attention for the treatment of tumors due to its obvious advantages. However, it still remains a challenge to determine the volume of frozen tissues in real-time, which greatly lowers the therapeutic efficacy of cryosurgery and hinders its broad application for the treatment of cancers. Herein, we report a freezing induced turn-on strategy for selective real-time imaging of frozen cancer cells. As a type of aggregation induced emission (AIE) fluorogen, TABD-Py molecules interact specifically with ice crystals and form aggregates at the ice/water interface. Consequently, bright fluorescent emission appears upon freezing. Note that TABD-Py molecules are enriched only in the cancer cells and exhibit high biocompatibility as well as low cytotoxicity, therefore a freezing induced turn-on imaging modality for cryosurgery is developed, which will certainly maximize therapeutic efficacy of cryosurgery in treating tumors.
关键词： aggregation induced emission,cancer cells,real-time imaging,freezing,cryosurgery
更新于2025-09-23 15:23:52