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A sensitive and selective fluorescence probe for detection of hypochlorite (OCl?) and its bioimaging in live cells
摘要： A novel indolium-based fluorescent probe (probe 1) for the recognition and detection of hypochlorite (OCl?) has been explored via a double oxidation reaction mechanism. Probe 1 exhibited excellent selectivity and sensitivity for OCl? over other analytes, and with a detection limit of 0.11 μM. Meanwhile, probe 1 showed fast response towards OCl? in less than 3 min with obvious changes in color, which could be observed by naked eye. Moreover, fluorescence imaging experiments by using Eca109 cells were performed utilizing the new probe, demonstrating its practical applications in living cells.
关键词： Oxidize,Fluorescence probe,Bioimaging,Hypochlorite
更新于2025-11-21 11:24:58
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Self-Assembled Nanostructures of Red Fluorescent Amphiphilic Block Copolymers as Both Imaging Probes and Drug Carriers
摘要： We report a red-?uorescent drug delivery system formed by biodegradable and biocompatible amphiphilic A-B-A block copolymers. Each polymer consists of a red ?uorescent dye covalently bonded in the middle of hydrophobic block (B) of polylactone, tethered at both ends with poly[(oligo ethylene glycol) methyl ether methacrylate] (POEGMA) as the hydrophilic block. Two types of polylactones, i.e., semicrystalline poly(ε-caprolactone) (PCL) and amorphous poly(δ-decalactone) (PDL), respectively, were incorporated as the hydrophobic segment in the block copolymers. Using transmission electron microscopy, we characterized the self-assembled nanostructures formed by these amphiphilic block copolymers in mixtures of water/tetrahydrofuran or water/dimethylformamide. All of these polymers remained highly ?uorescent in water, although some extent of aggregation-induced ?uorescence quenching was still observed. Among the three types of polymers presented here, the polymer (RPO-3) containing an amorphous block of PDL showed the highest drug-loading capacity and the largest extent of drug release in acidic media. RPO-3 micelles loaded with doxorubicin as a model of anticancer drug showed sustainable intracellular release and cytotoxicity against HeLa cells.
关键词： self-assembly,drug delivery,polymer,?uorescence,bioimaging
更新于2025-11-21 11:08:12
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Green Preparation of Fluorescent Carbon Quantum Dots from Cyanobacteria for Biological Imaging
摘要： Biomass-based carbon quantum dots (CQDs) have become a significant carbon materials by their virtues of being cost-effective, easy to fabricate and low in environmental impact. However, there are few reports regarding using cyanobacteria as a carbon source for the synthesis of fluorescent CQDs. In this study, the low-cost biomass of cyanobacteria was used as the sole carbon source to synthesize water-soluble CQDs by a simple hydrothermal method. The synthesized CQDs were mono-dispersed with an average diameter of 2.48 nm and exhibited excitation-dependent emission performance with a quantum yield of 9.24%. Furthermore, the cyanobacteria-derived CQDs had almost no photobleaching under long-time UV irradiation, and exhibited high photostability in the solutions with a wide range of pH and salinity. Since no chemical reagent was involved in the synthesis of CQDs, the as-prepared CQDs were confirmed to have low cytotoxicity for PC12 cells even at a high concentration. Additionally, the CQDs could be efficiently taken up by cells to illuminate the whole cell and create a clear distinction between cytoplasm and nucleus. The combined advantages of green synthesis, cost-effectiveness and low cytotoxicity make synthesized CQDs a significant carbon source and broaden the application of cyanobacteria and provide an economical route to fabricate CQDs on a large scale.
关键词： bioimaging,cyanobacteria,carbon quantum dots,hydrothermal method
更新于2025-11-21 11:08:12
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Microwave-Assisted Synthesis of Quasi-Pyramidal CuInS <sub/>2</sub> -ZnS Nanocrystals for Enhanced Near-Infrared Targeted Fluorescent Imaging of Subcutaneous Melanoma
摘要： Near-infrared (NIR) fluorescent CuInS2–ZnS nanocrystals (CIZS NCs) are synthesized via an ultra-fast, non-injection microwave (MW)-assisted nanoalloying process at 230 oC within 5 min using 1-dodecanethiol (DDT) as both the sulfur source and solvent under solvothermal (ST) condition. The structural and surface analyses reveal that DDT-functionalized CIZS NCs exhibit quasi-pyramids of tetragonal-phase with well-defined facets. The DDT-functionalized CIZS NCs present a photoluminescence quantum yield (PLQY) of 76% and a long-lived fluorescence lifetime of ≈0.6 μs in organic-phase. Subsequently, DDT-functionalized CIZS NCs are phase-transferred via ligand-exchange using 11-mercaptoundecanoic acid (MUA) into water-soluble MUA–CIZS NCs that exhibit a substantial PLQY of 55%. In addition, the NIR-fluorescent MUA-functionalized CIZS NCs in conjugation with folic acid (FA), as a tumor-targeting ligand, demonstrates enhanced tumor-targeted imaging ability. The FA–MUA–CIZS NC conjugates exhibit a cell viability of ≈75% even at the highest concentration of 1 mg mL–1 and a labeling efficiency of 95.4%. The in vivo imaging results corroborate that FA–MUA–CIZS NCs conjugates are actively targeted to folate receptor-positive B16F10 tumor-bearing C57BL/6 mice in 2 h. The histopathological and hematological studies confirm no significant changes in tissue architecture and blood biochemical parameters. The confocal microscopy studies reveal deep penetration and uniform distribution of FA–MUA–CIZS NCs conjugates in subcutaneous melanoma.
关键词： CuInS2–ZnS nanocrystals,nano-bioprobe,nanoalloying approach,targeted bioimaging,microwave-solvothermal method
更新于2025-11-21 11:08:12
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The Application of Green-Synthesis-Derived Carbon Quantum Dots to Bioimaging and the Analysis of Mercury(II)
摘要： Ginkgo leaves were used as precursors for the hydrothermal synthesis of carbon quantum dots (CQDs), which were subsequently characterized by transmission electron microscopy as well as Fourier-transform infrared, X-ray powder di?raction, and X-ray photoelectron spectroscopy. The prepared CQDs exhibited a ?uorescence quantum yield of 11% and superior water solubility and ?uorescence stability, as well as low cytotoxicities and excellent biocompatibilities with A549 and HeLa cells; these CQDs were also used to bioimage HeLa cells. Moreover, owing to the experimental observation that Hg2+ quenches the ?uorescence of the CQDs in a speci?c and sensitive manner, we developed a method for the detection of Hg2+ using this ?uorescence sensor. The sensor exhibited a linear range for Hg2+ of 0.50–20 μM, with an excellent coe?cient of determination (R2 ? 0.9966) and limit of detection (12.4 nM). In practice, the proposed method was shown to be highly selective and sensitive for the monitoring of Hg2+ in lake water and serum samples.
关键词： mercury(II) detection,carbon quantum dots,hydrothermal synthesis,fluorescence quenching,bioimaging
更新于2025-11-19 16:46:39
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Novel BODIPY-based fluorescent probes with large Stokes shift for imaging hydrogen sulfide
摘要： Hydrogen sulfide has important biomedical significance because it plays a regulatory role in a variety of physiological processes. In our previous work, we investigated the effect of substituents on Stokes shift of BODIPY and found that methoxy groups on phenyl substituents at 3,5-positions could expand the Stokes shift of BODIPY-based probes. In this study, taking hydrogen sulfide (H2S) as analyte, we designed and synthesized 4,4-difluoro-8-{4-(2,4-dinitrophenoxy)phenyl}-3,5-bis(2,4-dimethoxyphenyl)-4-bora-3a,4a-diaza-s-indancene (DMOEPB) and 4,4-difluoro-8-(4-nitrophenyl)-3,5-bis(2,4-dimethoxyphenyl)-4-bora-3a,4a-diaza-s-indancene (DMONPB) according to the same strategy. As expected, Stokes shifts of 49 nm and 51 nm were obtained respectively, which demonstrate the feasibility of the strategy in the synthesis of other fluorescent probes for the detection of small biomolecules. DMONPB can react with H2S to form the derivative with a fluorescence quantum yield of 0.13. An excellent linear relationship was observed in the range of H2S concentrations from 0 μM to 800 μM and the detection limit was 1.3 μM. Furthermore, it can be successfully applied to imaging of H2S in cells and tissues. For DMOEPB, the presence of m-dinitrophenyl did not cause the expected fluorescence quenching, which makes it impossible to perform bioimaging. We speculate that the strong electron-withdrawing property of nitro group is obviously weaker when the two benzene rings are separated at 8-position and are not conjugated with the fluorophore. It provides experience and lessons for designing the same type of turn-on probe.
关键词： hydrogen sulfide,BODIPY,red fluorescence,bioimaging,large Stokes shift
更新于2025-11-19 16:46:39
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Sulfur doped carbon nitride quantum dots with efficient fluorescent property and their application for bioimaging
摘要： Heteroatom doping can drastically alter electronic characteristics of carbon nitride quantum dots, thus resulting in unusual properties and related applications. Herein, we used sulfur as the doping element and investigated the influence of doping on the electronic distribution of carbon nitride and the corresponding fluorescent property. A simple synthetic strategy was applied to prepare sulfur-doped carbon nitride (S-g-C3N4) quantum dots through ultrasonic treatment of bulk S-g-C3N4. Characterization results demonstrated that the prepared S-g-C3N4 quantum dots with an average size of 2.0 nm were successfully prepared. Fluorescent properties indicated that S-g-C3N4 quantum dots have an emission peak at 460 nm and cover the emission spectra region up to 550 nm. Furthermore, the fluorescent intensity is greatly increased due to the sonication of bulk S-g-C3N4 into quantum dots. As a result, S-g-C3N4 quantum dots not only show a blue cell imaging, but have a bright green color. Therefore, S-g-C3N4 quantum dot is a promising candidate for bioimaging benefiting from the efficient fluorescent property, good biocompatibility, and low toxicity.
关键词： Doped carbon nitride,Fluorescent property,In vitro cytotoxicity,Quantum dots,Bioimaging
更新于2025-11-14 17:04:02
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Self-assembling Non-conjugated Poly(amide-imide) into Thermoresponsive Nanovesicles with Unexpected Red fluorescence for Bioimaging
摘要： Non-conjugated red fluorescent polymers have been increasingly studied to improve the biocompatibility and penetration depth over conventional fluorescent materials. However, the accessibility of such polymers remains challenging due to the scarcity of non-conjugated fluorophores and lacking relevant mechanism of red-shifted fluorescence. Herein, we discovered that the combination of hydrogen bonding and π-π stacking interactions provides non-conjugated poly(amide-imide) with a large bathochromic shift (> 100 nm) from blue-green fluorescence to red emission. The amphiphilic PEGylated poly(amide-imide) derived from in-situ PEGylation self-assembled into nanovesicles in water, which isolated the aminosuccinimide fluorophore from the solvents and suppressed the hydrogen bonds formation between aminosuccinimide fluorophores and water. Therefore, the fluorescence of PEGylated poly(amide-imide) in water was soundly retained. Furthermore, the strong hydrogen bonding and hydrophobic interactions with water provided PEGylated poly(amide-imide) with a reversible thermoresponsiveness and presented a concentration-dependent behavior. Finally, accompanied with the excellent biostability and photostability, PEGylated poly(amide-imide) exhibited as a good candidate for cell imaging.
关键词： poly(amide-imide),red-shifted fluorescence,self-assembly,bioimaging,nanovesicles
更新于2025-11-14 17:04:02
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Nanomechanical Microfluidic Mixing and Rapid Labelling of Silica Nanoparticles with Allenamide to Thiol Covalent Link for Bioimaging
摘要： Rapid surface functionalization of nanomaterials using covalent linkage following ‘green chemistry’ remains challenging and the quest for developing simple protocols are persisting. We report a nanomechanical microfluidic approach for the coupling of allenamide functionalized organic derivatives on the surface of thiol modified silica nanoparticles using allenamide-thiol chemistry. The coupling principle involves the use of a microfluidic surface acoustic wave (SAW) device that generates acoustic streaming based chaotic fluid micromixing that enables mixing of laterally flowing fluids containing active components. This approach was used to demonstrate the direct surface labeling of thiol modified silica nanoparticles using a selected group of modified fluorescence tags containing allenamide handles and achieved a total efficiency of 83-90 %. This green approach enabled a highly efficient surface functionalization in aqueous conditions, with tunable control over the conjugation process via the applied field. The dye labelled silica particles were characterized using various analytical techniques and found to be biocompatible with a potential in live cell bioimaging. It is envisaged that this bioconjugation strategy will find numerous applications in the field of bioimaging and drug delivery.
关键词： microfluidics,surface acoustic wave (SAW),allenamide-thiol chemistry,bioimaging,nanoparticles
更新于2025-09-23 15:23:52
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Enhancement strategies of targetability, response and photostability for in vivo bioimaging
摘要： Analyses of the physiology and pathology of active biochemical species in their native contexts are critical for early diagnosis and therapy. Optical imaging has emerged as one of the promising modalities for noninvasive and real-time visualization of important biomolecules or biological events, and it has witnessed major advances in the field of imaging in vitro and in vivo. In this review, we present a survey of common approaches and tactics for enhanced targetability, response rate, and photostability in bioimaging applications. Recently developed and representative examples are illustrated on the cellular and tissue levels.
关键词： targetability,bioimaging,photostability,response rate,fluorescent probe
更新于2025-09-23 15:23:52