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- 2017
- spectral reconstruction
- interference
- polarization
- transform
- imaging spectrometer
- Optoelectronic Information Science and Engineering
- BITTT
- Zhejiang University
- Guilin University of Aerospace Technology
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A mitochondria targetable and viscosity sensitive fluorescent probe and its applications for distinguishing cancerous cells
摘要： The determination of mitochondrial viscosity is of great importance owing to its crucial roles in the diffusion-mediated processes. It has demonstrated that the viscosity in cancer cells is higher than normal cells. Developing of viscosity sensitive fluorescent probes for distinguishing normal cells and cancer cells is necessary. Herein, we present a mitochondrial-targeting fluorescent probe PFV for the detection of viscosity in live cells. It exhibited outstanding sensitivity to viscosity, free from disturbing by ROS/sulfuret even at high concentrations. Equipped with mitochondria targeting ability, PFV was then applied to mitochondrial viscosity detection. Most importantly, by viscosity monitoring PFV successfully differentiated normal hepatic cells and cancerous hepatic cells. The present study would offer a broadly applicable for the determination of viscosity in complex systems.
关键词： fluorescence imaging,mitochondrial viscosity,fluorescent probe,cyanine dye,viscosity
更新于2025-11-14 15:29:11
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Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation
摘要： A turn-on fluorometric method is described for selective and sensitive detection of cysteine (Cys). Gold nanoclusters (Au NCs) capped with glutathione (GSH) are used as a fluorescent probe. If Ce3+ ion are present, they will bind to the carboxy groups of the GSH-capped Au NC. This results in aggregation-induced emission enhancement (AIEE), best measured at excitation/emission wavelengths of 360/575 nm. On addition of Cys, which has less steric hindrance compared with GSH and higher affinity for Ce3+, it will bind to Ce3+ through the carboxyl group and link with Au NCs via Au-S bond. Hence, the AIEE is increased and Cys can be quantified via this effect with a linear response in the 0.4–120 μmol L?1 Cys concentration range and a detection limit of 0.15 μmol L?1.
关键词： Au NC,HepG2 cell imaging,Aggregation-induced emission enhancement
更新于2025-11-14 15:26:12
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Tetramodal Imaging and Synergistic Cancer Radio-Chemotherapy Enabled by Multiple Component-Encapsulated Zeolitic Imidazolate Frameworks
摘要： The abundant species of functional nanomaterials have attracted tremendous interests as components to construct multifunctional composites for cancer theranostics. However, their distinct chemical properties substantially require a specific strategy to integrate them in harmony. Here, we report the preparation of a distinctive multifunctional composite by encapsulating small-sized semiconducting copper bismuth sulfide (CBS) nanoparticles and rare-earth down-conversion (DC) nanoparticles in larger-sized zeolitic imidazolate framework-8 (ZIF8) nanoparticles, followed by loading an anticancer drug, doxorubicin (DOX). Such composites can be used for tetramodal imaging, including traditional computed tomography and magnetic resonance imaging and, recently, for photoacoustic imaging and fluorescence imaging. With a pH-responsive release of the encapsulated components, synergistic radio-chemotherapy with a high (87.6%) tumor inhibition efficiency is achieved at moderate doses of the CBS&DC-ZIF8@DOX composite with X-ray irradiation. This promising strategy highlights the extending capacity of zeolitic imidazolate frameworks to encapsulate multiple distinct components for enhanced cancer imaging and therapy.
关键词： multifunctional,zeolitic imidazolate frameworks,tetramodal imaging,multiple encapsulation,synergistic radio-chemotherapy
更新于2025-11-14 15:24:45
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In Situ Synthesis of Fluorescent Mesoporous Silica–Carbon Dot Nanohybrids Featuring Folate Receptor-Overexpressing Cancer Cell Targeting and Drug Delivery
摘要： Multifunctional nanocarrier-based theranostics is supposed to overcome some key problems in cancer treatment. In this work, a novel method for the preparation of a fluorescent mesoporous silica–carbon dot nanohybrid was developed. Carbon dots (CDs), from folic acid as the raw material, were prepared in situ and anchored on the surface of amino-modified mesoporous silica nanoparticles (MSNs–NH2) via a microwave-assisted solvothermal reaction. The as-prepared nanohybrid (designated MSNs–CDs) not only exhibited strong and stable yellow emission but also preserved the unique features of MSNs (e.g., mesoporous structure, large specific surface area, and good biocompatibility), demonstrating a potential capability for fluorescence imaging-guided drug delivery. More interestingly, the MSNs–CDs nanohybrid was able to selectively target folate receptor-overexpressing cancer cells (e.g., HeLa), indicating that folic acid still retained its function even after undergoing the solvothermal reaction. Benefited by these excellent properties, the fluorescent MSNs–CDs nanohybrid can be employed as a fluorescence-guided nanocarrier for the targeted delivery of anticancer drugs (e.g., doxorubicin), thereby enhancing chemotherapeutic efficacy and reducing side effects. Our studies may provide a facile strategy for the fabrication of multifunctional MSN-based theranostic platforms, which is beneficial in the diagnosis and therapy of cancers in future.
关键词： Targeted drug delivery,Fluorescence imaging,Mesoporous silica nanoparticles,Carbon dots,Chemotherapy
更新于2025-11-14 14:48:53
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Illumination conditions in microsphere-assisted microscopy
摘要： White-light microsphere-assisted microscopy is a full-field and label-free imaging promising technique making it possible to achieve a sub-diffraction lateral resolution. However, performance of this technique depends not only on the geometrical parameters but also on the illumination conditions of the optical system. In the present work, experimental measurements and computer simulations have been performed in air in order to determine the influence of the two diaphragm apertures of the K?hler arrangement and the spectral width of the light source on both the depth-of-focus of the microsphere and the optimisation of the imaging contrast. Furthermore, the super-resolution phenomenon is demonstrated and the cumulated optical aberrations are shown through the measurement of the optical transfer function for the different arrangements of the illumination part.
关键词： Super-resolution microscopy,Illumination,Microsphere,Optical transfer function,Imaging depth
更新于2025-10-24 16:39:32
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System Design and Evaluation of a Compact and High Energy X-ray Talbot-Lau Grating Interferometer for Industrial Applications
摘要： X-ray grating interferometry has been an active area of research in recent years. In particular, various studies have been carried out for the practical use of the x-ray grating interferometer in medical and industrial fields. For the commercialization of the system, it needs to be optimized for its application. In this study, we have developed a prototype of the compact high energy x-ray grating interferometer of which the high effective energy and compactness is of our primary feature of design. We have designed the Talbot-Lau x-ray interferometer in a symmetrical geometry with an effective energy of 54.3 keV. The system has a source-to-analyzer grating distance of 788.4 mm, which is compact enough for a commercial product. In a normal operation, it took less than ten seconds to acquire a set of phase stepping images. The acquired images had a maximum visibility of about 15%, which is relatively high compared with the visibilities of the other high-energy grating interferometric systems reported so far.
关键词： X-ray phase contrast imaging,Dark-field imaging,Talbot-Lau interferometry,compact design,High energy phase contrast imaging
更新于2025-09-23 15:23:52
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Spectral correction for handheld optoacoustic imaging by means of near-infrared optical tomography in reflection mode
摘要： In vivo imaging of tissue/vasculature oxygen saturation levels is of prime interest in many clinical applications. To this end, the feasibility of combining two distinct and complementary imaging modalities was investigated: optoacoustics (OA) and near-infrared tomography (NIROT), both operating noninvasively in reflection mode. Experiments were conducted on two optically heterogeneous phantoms mimicking tissue before and after the occurrence of a perturbation. OA imaging was used to resolve submillimetric vessel-like optical absorbers at depths up to 25 mm, but with a spectral distortion in the OA signals. NIROT measurements were utilized to image perturbations in the background and to estimate the light fluence inside the phantoms at the wavelength pair (760 nm, 830 nm). This enabled the spectral correction of the vessel-like absorbers' OA signals: the error in the ratio of the absorption coefficient at 830 nm to that at 760 nm was reduced from 60%-150% to 10%-20%. The results suggest that oxygen saturation (SO2) levels in arteries can be determined with <10% error and furthermore, that relative changes in vessels' SO2 can be monitored with even better accuracy. The outcome relies on a proper identification of the OA signals emanating from the studied vessels.
关键词： fluence compensation,near-infrared optical tomography,blood oxygen saturation,quantitative optoacoustic imaging,optoacoustic signal quantification,multimodal imaging,spectral correction
更新于2025-09-23 15:23:52
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Underwater Imaging Based on LF and Polarization
摘要： The underwater image restoration based on polarization information has achieved good results in improving the image quality in scattering media. However, the previous methods are difficult to obtain the true distribution of degree of polarization in the scene. In this paper, we combine synthetic aperture imaging with polarimetric imaging, and propose a method for retrieving radiation of object based on the degree of polarization and intensity of backscattering at the multi-view image. In addition, compared with the previous methods, the proposed method can achieve simultaneous acquisition of 4D light field information and polarization information, effectively increasing the information dimension obtained by single imaging. In order to verify the effectiveness and superiority of the proposed method, we have established a relevant experimental platform and compared with the experimental results of the previous methods, and obtained the expected experimental results.
关键词： synthetic aperture imaging,polarimetric imaging,underwater image restoration
更新于2025-09-23 15:23:52
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Patient Factor Disparities in Imaging Follow-Up Rates After Incidental Abdominal Findings
摘要： OBJECTIVE. The effect of demographics and societal determinants on imaging follow-up rates is not clear. The purpose of this study was to compare characteristics of patients with imaging findings representing possible cancer who undergo follow-up imaging versus those who do not to better understand factors that contribute to follow-up completion. MATERIALS AND METHODS. The records of 1588 patients with indeterminate abdominal imaging findings consecutively registered between July 1, 2013, and March 20, 2014, were reviewed. Several patient characteristics, including distance between patients’ home zip codes and the flagship hospital of the health system were compared between the groups who did and did not undergo follow-up imaging. Subgroup analyses based on the location of the index examination were also performed. RESULTS. Among the 1513 (36.62%) included patients, 554 did not undergo follow-up abdominal imaging within 1 year of the index examination. The same was true of 270 of 938 (28.78%) outpatients and 168 of 279 (60.21%) emergency department patients. Eighty-nine of 959 (9.28%) patients who underwent follow-up imaging were younger than 40 years, compared with 76 of 554 (13.72%) patients who did not undergo follow-up imaging (p = 0.005). Fifty-four of 959 (5.63%) patients who underwent follow-up imaging were older than 80 years, compared with 70 of 554 (12.64%) patients who did not undergo follow-up imaging (p < 0.001). More white patients (587 of 959 versus 301 of 554, p = 0.007) and fewer black patients (204 of 554 versus 270 of 959, p < 0.001) were found in the follow-up imaging group. Greater distance from the flagship hospital correlated with less follow-up in the outpatient subgroup only (p = 0.03). CONCLUSION. Emergency department patients and patients at the extremes of age are less likely to complete follow-up imaging. Insurance status and race and ethnicity may affect follow-up completion rates. The relationship between distance to hospital and follow-up completion requires further investigation.
关键词： imaging follow-up,abdominal imaging,structured reports,incidental findings,actionable findings
更新于2025-09-23 15:23:52
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Radiomic analysis of imaging heterogeneity in tumours and the surrounding parenchyma based on unsupervised decomposition of DCE-MRI for predicting molecular subtypes of breast cancer
摘要： Objectives This study aimed to predict the molecular subtypes of breast cancer via intratumoural and peritumoural radiomic analysis with subregion identification based on the decomposition of contrast-enhanced magnetic resonance imaging (DCE-MRI). Methods The study included 211 women with histopathologically confirmed breast cancer. We utilised a completely unsupervised convex analysis of mixtures (CAM) method by unmixing dynamic imaging series from heterogeneous tissues. Each tumour and the surrounding parenchyma were thus decomposed into multiple subregions, representing different vascular characterisations, from which radiomic features were extracted. A random forest model was trained and tested using a leave-one-out cross-validation (LOOCV) method to predict breast cancer subtypes. The predictive models from tumoural and peritumoural subregions were fused for classification. Results Tumour and peritumour DCE-MR images were decomposed into three compartments, representing plasma input, fast-flow kinetics, and slow-flow kinetics. The tumour subregion related to fast-flow kinetics showed the best performance among the subregions for differentiating between patients with four molecular subtypes (area under the receiver operating characteristic curve (AUC) = 0.832), exhibiting an AUC value significantly (p < 0.0001) higher than that obtained with the entire tumour (AUC = 0.719). When the tumour- and parenchyma-based predictive models were fused, the performance, measured as the AUC, increased to 0.897; this value was significantly higher than that obtained with other tumour partition methods. Conclusions Radiomic analysis of intratumoural and peritumoural heterogeneity based on the decomposition of image time-series signals has the potential to more accurately identify tumour kinetic features and serve as a valuable clinical marker to enhance the prediction of breast cancer subtypes.
关键词： Magnetic resonance imaging,Diagnostic imaging,Breast neoplasms
更新于2025-09-23 15:23:52