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A New Generation of NIR-II Probes: Lanthanide-Based Nanocrystals for Bioimaging and Biosensing
摘要： Fluorescence-based imaging in the second near infrared window (NIR-II, 1000–1700 nm) is extensively used in both fundamental scientific research and clinical practice, owing to its advances of high sensitivity and high spatiotemporal resolution with increasing tissue penetration depths. Among several NIR-II fluorophores, recent accomplishments in biocompatible lanthanide-based luminescent nanomaterials have aroused great interest of researchers. This progress report summarizes recent progress in controlled synthesis of lanthanide-based NIR-II nanomaterials and their state-of-the-art in NIR-II biomedical imaging and biosensing applications. In addition, challenges and opportunities for this kind of novel NIR-II nanoprobes are also discussed.
关键词： lanthanides,bioimaging,NIR-II,near infrared imaging,fluorescence,nanoparticles
更新于2025-09-23 15:22:29
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Rhenium and technetium-complexed silicon rhodamines as near-infrared imaging probes for bimodal SPECT- and optical imaging
摘要： Fluorescent Si-rhodamines were modified to enable complexation with the Re(I)- and 99mTc(I)-tricarbonyl core. The corresponding complexes exhibit suitable properties as bimodal imaging probes for SPECT- and optical imaging in vitro. Importantly, the novel in aqueous solution stable, functionalized Si-rhodamines retain favourable optical properties after complexation (QY=0.09, λabs=654 nm, λem=669 nm in PBS) and show promising near-infrared optical properties for potential in vivo applications enabling bimodal scintigraphic imaging and optical imaging, e.g. used in radio- and fluorescence-guided tumor resection.
关键词： SPECT,Optical imaging,Near-infrared imaging,Technetium,Rhenium,Silicon rhodamines
更新于2025-09-23 15:21:01
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Near-infrared fluorescence-guided surgery using indocyanine green facilitates secure infrapyloric lymph node dissection during laparoscopic distal gastrectomy
摘要： Purpose We aimed to investigate the usefulness of near-infrared indocyanine green (ICG)-enhanced fluorescence guidance for infrapyloric LN dissection in laparoscopic distal gastrectomy. Methods This study enrolled patients with early gastric cancer scheduled for laparoscopic distal gastrectomy. After intra-operative submucosal injection of ICG (0.1 mg/mL), LN dissection was conducted under near-infrared ICG fluorescence guidance. The operation time, bleeding events during infrapyloric LN dissection were analyzed. Cases were retrospectively 1:3 propensity-score matched to patients who underwent laparoscopic distal gastrectomy without ICG injection. Results The mean time from midline omentectomy to exposure of the right gastroepiploic vein was significantly shorter in the ICG group (n = 20) than in the non-ICG group (n = 60) (13.05 ± 5.77 vs 18.68 ± 7.92 min; p = 0.001), and the incidence of bleeding during infrapyloric LN dissection was lower in the ICG group (20% vs 68.3%, p < 0.001). The two groups did not differ significantly regarding the number of LNs retrieved from the infrapyloric area (p = 0.434). Conclusions Near-infrared ICG fluorescence guidance facilitates safe and fast infrapyloric LN dissection in laparoscopic distal gastrectomy.
关键词： Indocyanine green,Laparoscopic surgery,Lymph node dissection,Near-infrared imaging,Gastric cancer
更新于2025-09-23 15:21:01
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A dual colorimetric and near-infrared fluorescent turn-on probe for Hg2+ detection and its applications
摘要： The increasing Hg2+ contamination in environment and ecosystem has gained wide attention and thus demands for its facile and effective detection. In this study, we constructed a new fluorescent probe (E)-2-(2-(3-(1,3-dithian-2-yl)-4-hydroxystyryl)-4H-chromen-4-ylidene)malononitrile (DCM-Hg) for Hg2+ detection by incorporating the 1, 3-dithiane group to the dicyanomethylene-4H-pyran fluorophore for the first time. This probe can selectively detect Hg2+ via the Hg2+-triggered deprotection reaction of thioacetals, which leads to a distinct color change from purple to pink and obvious fluorescence enhancement in the near-infrared (NIR) region. The NIR fluorescence intensity increased linearly with Hg2+ level within the range from 0 to 100 μM and the detection limit of DCM-Hg for Hg2+ was found to be 6.8 × 10-8 mol/L. Moreover, the probe could sense Hg2+ on paper strips and image Hg2+ in living cells with low cytotoxicity, which demonstrates its potential application in environment and biological science.
关键词： Selectivity,Near-infrared,Imaging,Color change,Hg2+
更新于2025-09-23 15:21:01
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IGCS Intraoperative Technology Taskforce. Update on near infrared imaging technology: beyond white light and the naked eye, indocyanine green and near infrared technology in the treatment of gynecologic cancers
摘要： Surgery, by its very nature, relies on the ability of the surgeon to visualize and distinguish healthy and non-healthy tissue or structures. Without advanced technology, surgeons can visualize only that which can be seen with the naked eye or using white light imaging. Critical information on tissues, anatomical structures and physiological processes remain hidden and difficult to discern. Fluorescence imaging augments the basic surgical information. This form of imaging entails injecting a contrast or fluorescence agent (fluorophore) that is then illuminated by the appropriate wavelength of light required to excite the fluorophore. The excited fluorophore emits light of a slightly longer wavelength that is selectively imaged to produce a fluorescence image. The first fluorescent agent used in surgery was an intravenous injection of fluorescein, where it was used to enhance intracranial neoplasms. One particular imaging agent, indocyanine green, has been a significant driver of adoption of fluorescence imaging. Indocyanine green was first developed in 1955 by Kodak Research Laboratories and was approved by the Food and Drug Administration (FDA) in 1959 for retinal angiography. Since that time, this fluorophore has been used for a variety of surgical applications due to its unique properties: relative non-toxicity, depth of visualization through tissue, and remaining confined to intravascular and lymphatic spaces due to binding predominately to lipoproteins. The large depth of visualization results from the fluorescence properties of bound indocyanine green which is optimally excited with 805 nm light and emits over an approximate wavelength range from 810 nm to 875 nm. These near infrared wavelengths, invisible to the naked eye, pass through tissue particularly well due to the low adsorption of light by the various structures of tissue, such as hemoglobin and water. As a result, the tissue is relatively transparent to this light and images of structures as much as 5 mm below the tissue surface can be formed. By comparison, fluorescence imaging with fluorescein images only 2–3 mm below the tissue surface; thus subsurface structures cannot be imaged using visible fluorophores.
关键词： gynecologic cancers,fluorescence imaging,indocyanine green,near infrared imaging,surgical technology
更新于2025-09-23 15:19:57
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Fluorescence-Guided Neurosurgery (Neuro-oncology and Cerebrovascular Applications) || 1 Current Fluorescence-Guided Neurosurgery and Moving Forward
摘要： This introductory chapter provides a current overview of fluorescence-guided neurosurgery and includes future directions. The concepts of fluorescence and fluorescence-guided surgery (FGS) are introduced. Currently used fluorescent contrast agents in patients are summarized, including 5-aminolevulinic acid (5-ALA), fluorescein, and indocyanine green. Excitation light sources are discussed for each fluorescent contrast agent. Targeted fluorophores under clinical development for FGS are also introduced. Future directions in fluorescence-guided neurosurgery including handheld devices to better detect tumor fluorescence, dual fluorophore imaging, metabolic imaging in combination with FGS, and detection of the tumor margin will be discussed.
关键词： near-infrared imaging,fluorophore,indocyanine green,fluorescence-guided neurosurgery,fluorescence,operative microscope,fluorescence-guided surgery,fluorescein sodium,5-ALA
更新于2025-09-19 17:15:36
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Ex-vivo Imaging of Upper Tract Urothelial Carcinoma Using Novel ICG-Var3 pHLIP Imaging Agent
摘要： Objectives: To improve visualization of upper tract urothelial carcinomas (UTUC). Previous studies using the novel pH low insertion peptide (pHLIP) variant 3 (Var3) conjugated to indocyanine green (ICG) have demonstrated high sensitivity and specificity for imaging of bladder urothelial carcinoma. Here, we describe a novel approach for the imaging of UTUC using ICG-Var3 pHLIP. Methods: Twelve ex-vivo upper urinary tract specimens were irrigated with ICG-Var 3 pHLIP for fifteen minutes and then examined using a white light laparoscopic camera followed by near infrared fluorescent (NIRF) imaging using a Stryker 1588 AIM imaging system. Standard histopathologic evaluation was performed and findings were correlated with white light and ICG-Var3 NIRF imaging. One patient who underwent radical nephrectomy for renal cell carcinoma was used as a negative control. Results: Nineteen lesions were identified on histopathologic evaluation in ten patients, including 82% high-grade urothelial carcinoma and 18% low-grade urothelial carcinoma. Nineteen (100%) malignant lesions were identified using NIRF imaging, while 15 (78.9%) lesions were identified using conventional white light examination. The sensitivity of ICG-Var3 pHLIP NIRF imaging was 100% compared to 78.9% white light examination. Both modalities are 100% specific. Benign collecting systems and ureters did not show uptake of the pHLIP construct. Conclusions: In this feasibility study, the ICG-Var3 pHLIP imaging agent demonstrated superior diagnostic performance compared to conventional white light examination. While additional studies are required for validation and in-vivo translation, pHLIP-based imaging represents a promising tool to improve the evaluation and management of upper tract urothelial carcinoma.
关键词： urothelial carcinoma,pHLIP,fluorescence,NIRF,near infrared imaging
更新于2025-09-19 17:15:36
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Near-Infrared Imaging with Second-Window Indocyanine Green in Newly Diagnosed High-Grade Gliomas Predicts Gadolinium Enhancement on Postoperative Magnetic Resonance Imaging
摘要： Purpose: Intraoperative molecular imaging with tumor-targeting fluorophores offers real-time detection of neoplastic tissue. The second window indocyanine green (SWIG) technique relies on passive accumulation of indocyanine green (ICG), a near-infrared fluorophore, in neoplastic tissues. In this study, we explore the ability of SWIG to detect neoplastic tissue and to predict postoperative magnetic resonance imaging (MRI) findings intraoperatively. Procedures: Retrospective data were collected from 36 patients with primary high-grade gliomas (HGG) enrolled as part of a larger trial between October 2014 and October 2018. Patients received systemic ICG infusions at 2.5–5 mg/kg 24 h preoperatively. Near-infrared fluorescence was recorded throughout the case and from biopsy specimens. The presence/location of residual SWIG signal after resection was compared to the presence/location of residual gadolinium enhancement on postoperative MRI. The extent of resection was not changed based on near-infrared imaging. Results: All 36 lesions demonstrated strong near-infrared fluorescence (signal-to-background = 6.8 ± 2.2) and 100 % of tumors reaching the cortex were visualized before durotomy. In 78 biopsy specimens, near-infrared imaging demonstrated higher sensitivity and accuracy than white light for diagnosing neoplastic tissue intraoperatively. Furthermore, near-infrared imaging predicted gadolinium enhancement on postoperative MRI with 91 % accuracy, with visualization of residual enhancement as small as 0.3 cm3. Patients with no residual near-infrared signal after resection were significantly more likely to have complete resection on postoperative MRI (p value G 0.0001). Conclusions: Intraoperative imaging with SWIG demonstrates highly sensitive detection of HGG tissue in real time. Furthermore, post-resection near-infrared imaging correlates with postoperative MRI. Overall, our findings suggest that SWIG can provide surgeons with MRI-like results in real time, potentially increasing resection rates.
关键词： Intraoperative MRI,Postoperative MRI,Indocyanine green,Near-infrared imaging,High-grade gliomas
更新于2025-09-16 10:30:52
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Sharp pH-responsive mannose prodrug polypeptide nanoparticles encapsulating a photosensitizer for enhanced near infrared imaging-guided photodynamic therapy
摘要： Mannose has been reported as a novel drug to kill cancer cells. The prodrug of mannose will promote its targeted delivery and enrichment at the tumor site and cancer cells. Here, a pH-sensitive polypeptide copolymer with a tertiary amine group has been prepared and a mannose molecule was conjugated to the polymer through the formation of a Schiff base. At the same time, an iodinated boron dipyrromethene (BDPI) photosensitizer with high singlet oxygen generation efficacy and near infrared (NIR) fluorescence was encapsulated by the nanoparticles, which makes it a potential pH-sensitive NIR imaging-guided chemotherapy/PDT agent. In vitro and in vivo studies reveal that in a tumor acidic environment, the protonation of the tertiary amine group destroyed the nanostructure of the nanoparticles, resulting in increased BDPI release. Meanwhile, the bond cleavage of the Schiff base led to the release of conjugated mannose and synergistic inhibition of tumor cell growth with the PDT effect was realized. The combination of these two kinds of tumor suppression effects and photodynamic therapy made this pH-sensitive polypeptide delivery system show great potential for further cancer therapy.
关键词： polypeptide nanoparticles,mannose prodrug,pH-responsive,photodynamic therapy,photosensitizer,near infrared imaging
更新于2025-09-16 10:30:52
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Near‐infrared imaging and optical coherence tomography for intraoperative visualization of tumors
摘要： Surgical excision is the foundation of treatment for early-stage solid tumors in man and companion animals. Complete excision with appropriate margins of surrounding tumor-free tissue is crucial to survival. Intraoperative imaging allows real-time visualization of tumors, assessment of surgical margins, and, potentially, lymph nodes and satellite metastatic lesions, allowing surgeons to perform complete tumor resections while sparing surrounding vital anatomic structures. This Review will focus on the use of near-infrared imaging and optical coherence tomography for intraoperative tumor visualization.
关键词： optical coherence tomography,tumors,intraoperative visualization,surgical excision,near-infrared imaging
更新于2025-09-11 14:15:04