研究目的
Developing a compact and inexpensive imaging device based on digital inline-holography for live-cell culture imaging inside a cell incubator environment, enabling automated cell counting and tracking.
研究成果
The utilization of inline-holographic microscopy for cell culture investigation proves to be a promising way of revealing cell growth and dynamics during incubation. The system is capable of long-term observation and acquisition of quantitative information like cell size, count, and mobility. Further optimization is needed to fully realize this approach's potential.
研究不足
The system requires further optimization in both hardware and software to fully unlock its potential. A cooling device was necessary due to the heated image sensor causing damage on observed cells.
1：Experimental Design and Method Selection:
A digital inline-holographic microscope was built using a multi-color LED light source, a pinhole, and a CMOS sensor. The image reconstruction was performed using the angular spectrum approach with custom software.
2：Sample Selection and Data Sources:
Polystyrene particles with various diameters and a USAF1951 resolution test chart were used for testing and calibration. Immortalized mouse astrocyte cells and a MIN6 cell line were investigated for online measurements.
3：List of Experimental Equipment and Materials:
ON Semiconductor MT9P031 CMOS sensor, multi-color LED light source, 90 μm pinhole, petri dish, 3D printed PMMA housing.
4：Experimental Procedures and Operational Workflow:
The system was placed inside a cell incubator for long-term operation, with measurements taken automatically every 10 min. Data was stored locally and streamed to a cloud storage device.
5：Data Analysis Methods:
Image reconstruction was performed using the angular spectrum approach. Cell counting was based on blob detection or artificial neural network approach.
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