研究目的
To develop a ratiometric fluorometric assay for the detection of silver(I) ions using a dually emitting quantum dot hybrid.
研究成果
The ratiometric fluorometric assay using N,Si-CQDs and NALC-CdTe QDs is highly selective and sensitive for Ag(I) detection, with potential applications in environmental monitoring. The fluorescence quenching mechanism involves the formation of AgTe and Ag-SR bonds on the QDs' surface.
研究不足
The method may have slight interference from Cu2+ ions, which can be mitigated with EDTA. Direct application in complex samples like wastewaters may require sample digestion to remove organic substances that absorb at the excitation wavelength.
1：Experimental Design and Method Selection:
The study employs a ratiometric fluorometric assay using a hybrid of blue-fluorescent silicon- and nitrogen-doped carbon quantum dots (CQDs) and red-emitting CdTe quantum dots (QDs) capped with N-acetyl-L-cysteine.
2：Sample Selection and Data Sources:
The assay was tested with surface water and wastewater samples.
3：List of Experimental Equipment and Materials:
Instruments included a Jem-1200 transmission electron microscope (TEM), AVATAR 370 FTIR spectrometer, SIRION-100 X-ray energy dispersive spectroscopy (EDS), F-2500 spectrofluorimeter, Cary 100 UV-Vis spectrophotometer, and MP 220 pH meter. Chemicals included N-acetyl-L-cysteine, 3-aminopropyl-triethoxysilane, sodium borohydride, tellurium powder, citric acid, cadmium chloride hydrate, silver nitrate, and others.
4：Experimental Procedures and Operational Workflow:
The N,Si-CQDs and NALC-CdTe QDs were synthesized and mixed to form a ratiometric fluorescent probe. The fluorescence intensity was measured at excitation and emission wavelengths of 350 nm and 441/608 nm, respectively.
5：Data Analysis Methods:
The relationship between the log of fluorescence intensity ratios and Ag(I) concentration was analyzed for linearity and detection limits.
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AVATAR 370 FTIR spectrometer
AVATAR 370
Thermo Nicolet
Recording Fourier transform infrared spectra (FTIR) in KBr
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F-2500 spectrofluorimeter
F-2500
Hitachi
Recording fluorescence spectra
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Cary 100 UV-Vis spectrophotometer
Cary 100
Agilent
Recording absorption spectra
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Jem-1200 transmission electron microscope
Jem-1200
JEOL
Characterization of the size and surface morphology of QDs
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SIRION-100 X-ray energy dispersive spectroscopy
SIRION-100
PEI
Elemental compositions and contents analysis of QDs
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MP 220 pH meter
MP 220
Mettler Toledo
pH measurements
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