研究目的
Investigating the development of a ratiometric fluorescent aptasensor for the detection of Ochratoxin A (OTA) based on energy transfer between [Ru(bpy)3]2+ and silica quantum dots.
研究成果
The developed ratiometric fluorescent aptasensor is highly sensitive and selective for OTA detection, with a low detection limit of 0.08 ng mL?1. It successfully applied to real flour samples, offering a potential platform for food safety monitoring.
研究不足
The method may require strict pretreatment for biological samples with strong background UV absorption and fluorescence at 350 nm. Additionally, synthetization or functionalization of QDs with more red-shift excitation wavelengths could be necessary to mitigate interference.
1：Experimental Design and Method Selection:
The study designed a ratiometric fluorescent aptasensor using [Ru(bpy)3]2+ and silica QDs for OTA detection. The aptamer for OTA was used to shield [Ru(bpy)3]2+ into MSN, which is released upon OTA presence due to aptamer conformational change.
2：Sample Selection and Data Sources:
Flour samples were used to validate the method's practical applicability.
3：List of Experimental Equipment and Materials:
Materials included [Ru(bpy)3]2+, silica QDs, MSN, OTA aptamer, and various chemicals for preparation and assay. Equipment included a spectrofluorometer for fluorescence measurements.
4：Experimental Procedures and Operational Workflow:
The process involved preparing and loading aFMSN with [Ru(bpy)3]2+, mixing with OTA, and measuring fluorescence changes due to energy transfer.
5：Data Analysis Methods:
The ratio of fluorescent intensities at 625 nm and 442 nm was used for quantitative analysis of OTA concentration.
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