研究目的
Designing a novel, selective and sensitive fluorescent probe for the real-time detection of quercetin in red wine samples.
研究成果
The proposed SGQDs based fluorescent probe offers significant value for quercetin detection in real sample with good accuracy and high precision. The method is expected to offer a considerable implication to detect other antibodies, biomarkers, or aptamers by partially modifying the sensing molecule and for further exploration in clinical and pharmaceutical assay of quercetin for real-world application.
研究不足
The study focuses on the detection of quercetin in red wine samples and may not be directly applicable to other types of samples without further optimization.
1：Experimental Design and Method Selection:
SGQDs were synthesized by pyrolyzing citric acid (CA) and 3-Mercaptopropionic acid (MPA) and characterized through advanced techniques. The fluorescence intensity of SGQDs was quenched by the addition of quercetin through inner filter effect (IFE) mechanism.
2：Sample Selection and Data Sources:
Red wine samples with different quercetin concentrations were used for quantitative analysis.
3：List of Experimental Equipment and Materials:
Citric acid (CA), 3-Mercaptopropionic acid (MPA), sodium hydroxide (NaOH), quercetin, luteolin, apigenin, hesperetin, genistein, daidzein, malvidin, myricetin, kaempferol, phosphate buffered saline (PBS), ethanol solution.
4：Experimental Procedures and Operational Workflow:
SGQDs were synthesized, characterized, and used as a fluorescent probe for quercetin detection. The effect of pH, solvent, incubation time, concentration of quercetin and other co-existing flavonoid compounds on fluorescence intensity of SGQDs were discussed.
5：Data Analysis Methods:
The fluorescence intensity of SGQDs was measured with varying quercetin content, and the data was analyzed for linearity and detection limit.
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