研究目的
To analyze hair damage and support a claim of hair protection using a simple fluorescence method based on the emissive properties of damaged hair.
研究成果
The emissive properties of hair in the visible range can be used as a tool for the evaluation of hair damage and protection, offering a simple method for the cosmetic industry to substantiate claims of hair protection products.
研究不足
The study focused on the visible and UVA regions for hair damage analysis, potentially overlooking other wavelengths' effects. The method's effectiveness may vary with different hair types and colors.
1：Experimental Design and Method Selection:
The study used fluorimetry and microscopic procedures to observe hair fluorescence, comparing native hair with hair damaged by UVA and visible light.
2：Sample Selection and Data Sources:
Brazilian brown and white hair shafts were donated by human subjects, washed with neutral shampoo, and dried at room temperature before use.
3：List of Experimental Equipment and Materials:
Included deuterated water, tyrosinase, L-dopa, hydrochloric acid, phosphate buffer, and chamomile extract.
4：Experimental Procedures and Operational Workflow:
Hair shafts were exposed to UV and visible radiation in custom-made photoreactors, treated with chamomile extract, and analyzed for fluorescence changes.
5：Data Analysis Methods:
Spectroscopic properties were analyzed using a fiber-guided spectrophotometer and confocal microscopy, with data processed using Origin 8.0 Software and Image J Software.
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Axiovert 200 Microscope
Axiovert 200
Carl Zeiss
Used to obtain microscopic images of hair samples.
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Power Shot G10 Camera
G10
Canon
Used to register photographs of hair samples.
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USB-2000 Spectrophotometer
USB-2000
Ocean Optics
Used to acquire the reflectance spectra of the hair shafts.
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Edinburg F900 instrument
F900
Edinburgh
Used to measure emission intensities for singlet oxygen spectra.
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Photomultiplier R5509
R5509
Hamamatsu Photonics
Used to detect singlet oxygen emissions at 1270 nm.
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Edinburgh Instrument FLS 920
FLS 920
Edinburgh Instrument
Used for processing singlet oxygen emissions.
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Deuterated water
Sigma-Aldrich
Used as received in the study.
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Tyrosinase
Sigma-Aldrich
Used in melanin synthesis.
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L-dopa
Sigma-Aldrich
Used in melanin synthesis.
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Hydrochloric Acid
LabSynth
Used in the study.
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Phosphate buffer
LabSynth
Prepared with NaH2PO4 and Na2HPO4 for the study.
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Eppendorf Centrifuge
5804 R
Eppendorf
Used for centrifuging particle suspension in melanin synthesis.
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Surelite III Laser
Surelite III
Continuum
Used to excite hair shafts for singlet oxygen emission spectra.
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OPO Rainbow Laser
Rainbow
Quantel Brilliant
Used to excite melanin solutions for action spectra.
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Image J Launcher
NIH
Used for picture analyses.
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