摘要： This study presents a one-station 3D fabrication technique of nano?brous scaffold for tissue engineering. A divergence static electric ?eld was introduced in an electrospinning system to induce a self-assembly of aligned polycaprolactone (PCL) nano?bers into a tunable 3D architecture with thickness ranging from 2 to 12 mm. Silver nanoparticles were incorporated into the PCL solution to alter the electrical conductivity. Human ?broblast cells were cultured on the pure PCL nano?ber scaffolds in vitro for 7 days. It was found that the occurrence of nano?ber bridging phenomenon depended on the solution viscosity. The minimum viscosity to form a 3D nano?ber structure was higher than that to form a 2D nano?ber mat. The homogeneity of nano?ber distribution within the 3D space was positively correlated with the electrical conductivity and the weight of the nano?bers. In the cell culture test, ?broblasts proliferated on the scaffold and organized as an aligned matrix which mimicked the microstructure of native musculoskeletal tissues.