研究目的
To understand the interaction between citrate-coated silver nanoparticles (AgNPs) and gamma globulin (γ-globulin) using spectroscopic methods, aiming to provide insights into the biomedical applications of AgNPs.
研究成果
Citrate-coated AgNPs can bind to γ-globulin with high affinity, primarily through van der Waals interactions and hydrogen bonds, inducing slight conformational changes in γ-globulin. The fluorescence quenching mechanism is static. These findings contribute to understanding the biomedical applications of AgNPs.
研究不足
The study focuses on citrate-coated AgNPs and their interaction with γ-globulin, which may not represent interactions with other types of nanoparticles or proteins. The experimental conditions (e.g., temperature, pH) were controlled, which might differ from physiological conditions.
1：Experimental Design and Method Selection:
The study employed UV-vis absorption spectroscopy, fluorescence spectroscopy, fluorescence lifetime measurement, synchronous fluorescence spectroscopy, 3D fluorescence spectroscopy, and CD spectroscopy to investigate the interaction between AgNPs and γ-globulin.
2：Sample Selection and Data Sources:
γ-globulin was purchased from Sigma Co. (USA). AgNPs were synthesized and characterized in the lab.
3：List of Experimental Equipment and Materials:
Instruments used include a JEOL JEM-2100 (HR) electron microscope, Shimadzu XRD-6100 instrument, LS-55 spectrofluorimeter, and FS980 fluorescence spectrophotometer.
4：Experimental Procedures and Operational Workflow:
AgNPs were synthesized by a 'seed grow' method. The interaction studies were conducted by mixing γ-globulin with varying concentrations of AgNPs and analyzing the mixtures using the aforementioned spectroscopic methods.
5：Data Analysis Methods:
Data were analyzed using Stern-Volmer equation, Benesi and Hildebrand equation, and van’t Hoff equation to determine binding constants, quenching mechanisms, and thermodynamic parameters.
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High resolution transmission electron microscopy
JEOL JEM-2100 (HR)
JEOL
Recording TEM images of AgNPs
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Inductively coupled plasma spectrometry
ICPS-7500
Shimadzu
Determining the concentration of AgNPs
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X-ray diffraction measurements
Zetasizer NanoZS90
Malvern Instruments
Measuring XRD patterns of AgNPs
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Electron microscope FESEM-EDX
Carl Zeiss Sigma 300
Carl Zeiss Microscopy GmbH
EDX analysis of AgNPs
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Spectrofluorimeter
LS-55
Perkin-Elmer Co.
Recording fluorescence spectra of γ-globulin and AgNPs
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Spectrophotometer
TU-1901
Puxi Ltd
Gaining the absorption spectra of γ-globulin and AgNPs
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Fluorescence spectrophotometer
FS980
Edingburgh Instruments
Fluorescence lifetime measurement
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Circular Dichroism Photomultiplier
Applied Photophysics Limited
CD measurement of γ-globulin and AgNPs
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