研究目的
To develop a selective and sensitive fluorescent probe for the quantification of creatinine (CRN) using glutathione-capped copper nanoclusters (CuNCs) with aggregation-induced emission (AIE) characteristics.
研究成果
The study successfully developed a highly sensitive fluorescent probe for CRN detection using CuNCs with AIE characteristics. The probe demonstrated excellent selectivity and sensitivity, with potential applications in clinical diagnostics for renal dysfunction.
研究不足
The synthesis of nanoparticles to have better control over particle size and surface properties is difficult. The assay requires substantial skills for reproduction. Point-of-care determination of CRN by this method is challenging.
1：Experimental Design and Method Selection:
The study utilized the AIE characteristics of CuNCs for CRN detection. The methodology involved the synthesis of CuNCs, their characterization, and the evaluation of their fluorescence properties in the presence of Al3+ ions and CRN.
2：Sample Selection and Data Sources:
Human serum samples were used for real sample analysis. The samples were spiked with known concentrations of CRN to evaluate the method's accuracy and recovery.
3：List of Experimental Equipment and Materials:
Equipment included a Shimadzu RF-5301PC Spectrofluorimeter, Shimadzu UV2550 spectrophotometer, Zeiss TEM, and Tensor-27 FT-IR spectrometer. Materials included glutathione, copper sulfate, aluminum nitrate, and creatinine.
4：Experimental Procedures and Operational Workflow:
The procedure involved the preparation of CuNCs, testing their AIE characteristics with Al3+ ions, and then using these aggregates for CRN detection by observing fluorescence quenching.
5：Data Analysis Methods:
The fluorescence intensity was measured and analyzed to determine the concentration of CRN. The Stern-Volmer equation was used to establish the relationship between fluorescence quenching and CRN concentration.
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