1：Experimental Design and Method Selection:

The study involved measuring fluorescence lifetimes of dyes in solution to investigate concentration dependence, using spectroscopic techniques and exponential fitting of decay curves.

2：Sample Selection and Data Sources:

Dye 1 (CAS RN 110590-84-6) was synthesized, and Nile Blue A (CAS RN 3625-57-8) was purchased; solvents were spectroscopical grade chloroform and ethanol.

3：List of Experimental Equipment and Materials:

UV/Vis spectrometer (Varian Cary 5000), fluorescence spectrometer (Varian Cary Eclipse), light source (NKT-Laser SuperK Extreme EXB-4), monochromator (Edinburgh Instruments Ltd.), detector (PicoHarp 300 with PMA-C 192-N-M photomultiplier), quartz cuvettes.

4：Experimental Procedures and Operational Workflow:

Solutions were prepared at various concentrations; UV/Vis and fluorescence spectra were recorded, and fluorescence lifetimes were measured with excitation and detection at specific wavelengths, followed by exponential fitting.

5：Data Analysis Methods:

Fluorescence decays were deconvoluted and fitted exponentially; concentration dependencies were analyzed using equation (5) for solvent effects.