研究目的
To modify the surface of a titanium implant by a straightforward and cost-effective processing technique and to determine its effect on osteoblast differentiation.
研究成果
Hydrothermal treatment successfully created ZnO nanostructures on acid-etched Ti, enhancing osteoblast proliferation and ALP activity in a dose-dependent manner. This suggests a promising surface modification method for improving implant osseointegration, with potential for future in vivo studies and clinical applications.
研究不足
The study is limited to in vitro tests with SaOS-2 cells; in vivo performance and long-term effects are not evaluated. The hydrophilicity and topography were similar across groups, so other factors might influence results. Optimization of Zn concentration and nanostructure size could be further explored.
1：Experimental Design and Method Selection:
The study used hydrothermal treatment to incorporate Zn into acid-etched pure Ti surfaces, with varying Zn precursor concentrations (0.0002 M, 0.002 M, 0.02 M) to create ZnO nanostructures. The rationale was to enhance bioactivity for bone regeneration.
2：0002 M, 002 M, 02 M) to create ZnO nanostructures. The rationale was to enhance bioactivity for bone regeneration. Sample Selection and Data Sources:
2. Sample Selection and Data Sources: Pure titanium discs (grade 2) were used as samples. Human osteosarcoma cell line SaOS-2 cells were cultured for in vitro tests.
3：List of Experimental Equipment and Materials:
Equipment includes SEM (Merlin Compact, Zeiss), contact angle meter (JC2000C1; Powereach), XRD (Rigaku), XPS (Escalab 250Xi, Thermo Scientific), ICP-AES (IRIS Advantage ER/S; Thermo Fisher Scientific), microplate reader (Enspire, PerkinElmer). Materials include zinc acetate, ammonia solution, sulfuric acid, DMEM medium, McCoy's 5A medium, foetal bovine serum, penicillin-streptomycin, CCK-8 kit, ALP kit, BCA protein assay kit.
4：Experimental Procedures and Operational Workflow:
Samples were abrasive to 800-grit, cleaned ultrasonically, acid-etched in sulfuric acid, then hydrothermally treated in ammonia solution with Zn precursor at 95°C for 4 hours. Characterizations included SEM, contact angle measurement, XRD, XPS, Zn ion release analysis, cell proliferation (CCK-8), and ALP activity tests.
5：Data Analysis Methods:
Statistical analysis was performed (mean ± SD, n=3), with p-values indicating significance. Data were normalized for protein content in ALP tests.
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SEM
Merlin Compact
Zeiss
To examine the surface morphology of samples.
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XPS
Escalab 250Xi
Thermo Scientific
To analyze surface chemical states.
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ICP-AES
IRIS Advantage ER/S
Thermo Fisher Scientific
To analyze Zn2+ concentration in medium.
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Microplate Reader
Enspire
PerkinElmer
To examine cell proliferation using CCK-8 reagent.
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Contact Angle Meter
JC2000C1
Powereach
To evaluate hydrophilicity through contact angle measurements.
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XRD
Rigaku
To identify the composition of nanostructures.
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Pure Titanium Discs
Grade 2
Fgetting Metal Materials
Used as the base material for surface modification.
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Autoclave
20 mL capacity
Used for hydrothermal treatment.
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