研究目的
Improve mapping and registration of longitudinal view on histopathology vessels in a three-dimensional alignment procedure for postmortem quantitative coronary plaque analyses.
研究成果
The 3D fusion approach enabled slice-based comparison, showing that PMCTA overestimates calcified plaques and OCT underestimates them compared to histopathology. This provides a basis for improving automatic assessment of plaque constitution in future studies.
研究不足
Only two arteries from one heart were included; only 16 OCT images from LAD were of sufficient quality; postmortem settings lack dynamic movement and have deviant pressures, affecting results; annotations were not corrected for errors.
1：Experimental Design and Method Selection:
A 3D alignment procedure was developed to fuse histopathology, PMCTA, and OCT images for quantitative coronary plaque analysis. The procedure involved cropping and aligning histopathology slices, annotating landmarks, and constructing 3D volumes for comparison.
2：Sample Selection and Data Sources:
Data from two coronary arteries (LAD and RCA) from one postmortem heart were used. The heart was placed in a chest phantom (Kyoto Kagaku Lungman).
3：List of Experimental Equipment and Materials:
CT scanner (GE Healthcare CT750 HD), OCT system (Ilumien PCI optimization system from LightLab Imaging, St. Jude Medical), histopathology staining (hematoxylin and eosin), annotation software (QAngio CT Research Edition v
4：1, QCU-CMS-Research v4, custom histopathology annotation program, NPD view2 v1), and various catheters and guidewires. Experimental Procedures and Operational Workflow:
PMCTA and OCT acquisitions were performed, followed by histopathology analysis. Images were processed and annotated independently, then aligned using the 3D fusion procedure with manual landmark matching. Calcium quantification was done semi-automatically.
5：Data Analysis Methods:
Quantitative analysis of calcified plaque areas was performed, comparing modalities using mean areas along the vessel.
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Image viewing software
NPD view2 version 2.3.1
Hamamatsu Photonics
Used for viewing high-resolution histopathology images when closer examination is needed.
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CT scanner
CT750 HD
GE Healthcare
Used for post-mortem computed tomography angiography (PMCTA) to acquire images of coronary arteries.
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OCT system
Ilumien PCI optimization system
LightLab Imaging, St. Jude Medical
Used for optical coherence tomography (OCT) to acquire high-resolution images of coronary arteries.
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Plaque analysis software
QAngio CT Research Edition version 2.1.11.1
Medis medical imaging systems bv
Used for semi-automatic quantitative analysis of calcified plaques in PMCTA images.
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OCT analysis software
QCU-CMS-Research v4
Leiden University Medical Center
Used for processing and analyzing OCT images, including calcium annotation.
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Histopathology annotation program
Custom program for annotating structures in histopathology images, allowing freehand drawing and export to Excel.
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Chest phantom
Lungman
Kyoto Kagaku
Used to hold the postmortem heart during imaging to simulate in vivo conditions.
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