1：Experimental Design and Method Selection:

The study involved microbial synthesis of Functional-PHA using engineered Halomonas bluephagenesis, followed by chemical modifications via thiol-ene click reaction to graft N-acetyl-L-cysteine (NAL) onto PHA, and coordination with rare earth ions (Eu3+ and Tb3+). Theoretical models include coordination chemistry for metal chelation and photoluminescence principles.

2：Sample Selection and Data Sources:

Samples included Functional-PHA, NAL-grafted-PHA, and rare earth-chelated PHAs. Data were obtained from bacterial fermentation and chemical synthesis processes.

3：List of Experimental Equipment and Materials:

Equipment includes rotary shaker (Multitron-Pro, INFORS, Switzerland), Soxhlet extractor (Soxtec 2050, Foss, Denmark), NMR instrument (JNM-ECA600, JEOL, Japan), GPC (LC-20A with GPC-804C column, SHIMADAZU, Japan), FT-IR spectrometer (Bruker VERTEX70, Horiba, Germany), UV-Vis spectrophotometer (UV-3600, SHIMADAZU, Japan), spectrofluorometer (FP-6500, Jasco, Japan), fluorescence spectrometer (FLS980, Edinburgh Instruments, UK), DSC (DSC-Q20, TA, USA), TGA (TGA-50, SHIMADAZU, Japan), contact angle system (OCA15Pro, DataPhysics, Germany), microplate reader (Varioskan Flash, Thermo-Fisher Scientific, USA), and inverted fluorescence microscope (OLYMPUS IX83, Japan). Materials include chloroform, THF, methanol, ethanol, NAL, DMPA, DMSO, PLLA, rare earth nitrates, and cell culture reagents.

4：Experimental Procedures and Operational Workflow:

Steps include bacterial fermentation for PHA production, purification via extraction and precipitation, thiol-ene reaction under UV light, coordination reaction with rare earth ions, film preparation by solvent casting, and characterization using NMR, GPC, FT-IR, UV-Vis, luminescence studies, DSC, TGA, contact angle measurements, cell viability assays, and cell morphology observation.

5：Data Analysis Methods:

Data were analyzed using OPUS 7.0 software for FT-IR, standard calibration for GPC, and statistical methods for cell viability assays.