研究目的
Designing and preparing a novel rhodamine-based chemosensor for the dual-channel detection of Cu2+ and Co2+/trivalent metal ions, and investigating its aggregation-induced ratiometric emission activities.
研究成果
The developed sensor 1 is effective for detecting Co2+ and trivalent ions in CH3OH, Al3+ in H2O, and Cu2+ colorimetrically. It exhibits AIRE activities and can be used for intracellular Al3+ imaging, offering potential applications in environmental and biological monitoring.
研究不足
The sensor's response to Co2+ is disturbed by other metal ions like Cu2+, Ni2+, and trivalent cations in CH3OH. In H2O, it is selective for Al3+, but performance may vary in complex real-world samples. The AIRE phenomenon is solvent-dependent and may not be stable under all conditions.
1：Experimental Design and Method Selection:
The study involved designing and synthesizing a rhodamine hydrazone sensor (compound 1) by condensation reaction. UV-vis and fluorescence spectroscopy were used to study interactions with metal ions. AIRE properties were investigated in mixed solvents. DFT calculations were performed for structural insights.
2：Sample Selection and Data Sources:
Metal ions tested include Ag+, Al3+, Ca2+, Cd2+, Co2+, Cr3+, Cu2+, Fe3+, Hg2+, K+, Mg2+, Mn2+, Na+, Ni2+, Pb2+, Zn2+. Solutions were prepared in CH3OH and H2O.
3：2+. Solutions were prepared in CH3OH and H2O. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Instruments included UV-vis spectrophotometer, fluorescence spectrometer, TEM, DLS, ESI-MS, NMR spectrometer, and confocal microscope. Materials involved rhodamine 6G hydrazide, coumarin derivative, and various metal salts.
4：Experimental Procedures and Operational Workflow:
Synthesis of compound 1 via condensation. Titration experiments with metal ions in different solvents. Measurement of absorption and emission spectra. TEM and DLS for aggregation studies. Cell imaging with HeLa cells.
5：Data Analysis Methods:
Data analyzed using linear regression for detection limits, Job's plot for stoichiometry, Benesi-Hildebrand for association constants, and DFT for molecular geometry.
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UV-vis spectrophotometer
Measuring absorption spectra of solutions
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Fluorescence spectrometer
Measuring emission spectra of solutions
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TEM
Imaging morphology of aggregates
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DLS
Measuring particle size distribution
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ESI-MS
Mass spectrometry for molecular analysis
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NMR spectrometer
Nuclear magnetic resonance for structural analysis
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Confocal microscope
Fluorescence imaging in live cells
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DFT software
Gaussian 09
Gaussian
Density functional theory calculations for molecular geometry
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