研究目的
To develop a boron-doped carbon dots (B-CD) as a multifunctional fluorescent probe for the detection of sorbate (PS) and vitamin B12 (VB12) in food and pharmaceutical samples, addressing the need for an economical, fast, and accurate method.
研究成果
The B-CD probe is effective for sensitive and selective detection of PS and VB12 with low detection limits and good linear ranges. It was successfully applied to real samples with high recovery and low RSD. Future work should focus on improving selectivity, especially against food colorants, and expanding applications.
研究不足
The probe requires working in the UV region, making it prone to interferences from background fluorescence and UV-absorbing molecules. Food colorants can quench fluorescence and affect accuracy. The method may not be suitable for all real samples due to potential interferences.
1：Experimental Design and Method Selection:
A one-step hydrothermal method was used to synthesize B-doped carbon dots (B-CD) from phenylboronic acid. The quenching mechanism (mainly inner filter effect and possibly FRET) was investigated for detecting PS and VB12 via fluorescence spectroscopy.
2：Sample Selection and Data Sources:
Real samples including bread, vinegar, mineral water, vitamin drink, and VB12 tablets were purchased locally and pretreated (e.g., drying, milling, ultrasonic extraction, centrifugation, filtration) to prepare stock solutions for analysis.
3：List of Experimental Equipment and Materials:
Materials included phenylboronic acid (PBA, 97%), potassium sorbate (PS, AR), vitamin B12 (VB12, AR), Britton-Robinson buffer, ultrapure water, and other analytical grade reagents. Equipment details are in supporting information (not provided in paper).
4：Experimental Procedures and Operational Workflow:
Synthesis involved dissolving PBA in water, hydrothermal treatment at 200°C for 10h, centrifugation, filtration, dialysis, and drying. For detection, B-CD was mixed with PS/VB12 in buffer (pH 7.0), incubated for 1 min, and fluorescence spectra recorded (λex=247 nm, λem=323 nm). Selectivity and interference tests were conducted with various substances.
5：0), incubated for 1 min, and fluorescence spectra recorded (λex=247 nm, λem=323 nm). Selectivity and interference tests were conducted with various substances.
Data Analysis Methods:
5. Data Analysis Methods: Linear calibration curves (F0/F vs. concentration) were used for quantification. Detection limits calculated as 3σ/slope. Statistical analysis included recovery tests and RSD calculations for method validation.
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