1：Experimental Design and Method Selection:

The study involved synthesizing a hydrophobic derivative of indocyanine green (DCC) and encapsulating it into a pH-responsive copolymer (PDCZP) to form nanocarriers (DCC@PDCZP). Methods included UV-vis spectroscopy, fluorescence spectroscopy, TEM, DLS, flow cytometry, in vitro cell studies (MTT assay, cellular uptake), and in vivo studies on mouse models of melanoma and breast cancer with intratumoral injection and light irradiation.

2：Sample Selection and Data Sources:

B16-F10 mouse melanoma cells and 4T1 mouse breast cancer cells were used. Female BALB/c nude mice were inoculated with these cells to create orthotopic tumor models.

3：List of Experimental Equipment and Materials:

Equipment included spectrophotometers (TU-1901, Cary Eclipse), HPLC (Agilent 1260), TEM (Hitachi H-7650), DLS (Zetasizer Nano ZS), flow cytometer (BD FACS Calibur), confocal microscope (Nikon TiE-A1), infrared thermal camera (THERMOVISION A40), in vivo imaging system (IVIS Lumina), and laser emitters (830 nm and 660 nm). Materials included chemicals like DCC, PDCZP, mitoxantrone, cell culture reagents, and dialysis bags.

4：Experimental Procedures and Operational Workflow:

Synthesis of DCC and preparation of DCC@PDCZP nanocarriers; characterization of physical and photochemical properties; in vitro studies on cell viability, uptake, and localization; in vivo studies involving intratumoral injection, thermal and fluorescence imaging, and therapeutic efficacy assessment with histopathology and immunohistochemistry.

5：Data Analysis Methods:

Statistical analysis using SPSS 16.0 with ANOVA and LSD tests; data expressed as mean ± SD; significance levels at p<0.05 or p<0.01.