1：Experimental Design and Method Selection:

The study involved synthesizing a poly(azomethine-urethane) (PAMU) and preparing a composite with zeolite to enhance functional groups and interaction with DNA. Fluorescence spectroscopy was used for detection due to its sensitivity and selectivity.

2：Sample Selection and Data Sources:

Calf thymus DNA (Type I) was used as the DNA source. Materials such as (3-aminopropyl)trimethoxysilane (APTMS), 2,4-dihydroxy benzaldehyde (2,4-DHBA), lysine diisocyanate ethyl ester (LDI), and sodium zeolite (Y-zeolite) were commercially sourced.

3：List of Experimental Equipment and Materials:

Equipment included Perkin Elmer FT-IR Spectrum one, Bruker AC FT-NMR spectrometer, Shimadzu VP-10A GPC, Perkin-Elmer LS 55 fluorescence spectrometer, Schimadzu UV-1800 UV-Vis spectrophotometer, Zeiss EVO LS 10 SEM, and ultrasonic bath sonicator. Materials included various chemicals and solvents like DMF, EtOH, THF, MeCN, and PBS buffer.

4：Experimental Procedures and Operational Workflow:

Synthesis of prepolymer via condensation reaction, synthesis of PAMU via step-polymerization, preparation of PAMU-zeolite composite, characterization using FT-IR, NMR, GPC, SEM, fluorescence and UV-Vis measurements, optimization of parameters (composite concentration, pH, incubation time), DNA detection experiments, interference studies, and analytical applications.

5：Data Analysis Methods:

Fluorescence intensity differences (ΔF) were used to calibrate DNA concentration, with LOD calculated using standard deviation and slope from calibration curve. Statistical analysis included recovery and RSD calculations for synthetic samples.