研究目的
To develop and evaluate chemosensors L1 and L2 for selective detection of Hg2+ ions using various spectral techniques, and to explore their applications in molecular logic gates, keypad locks, live cell imaging, and real water sample analysis.
研究成果
The chemosensors L1 and L2 effectively detect Hg2+ ions with high selectivity, reversibility with EDTA, and low detection limits. They enable applications in molecular logic gates, keypad locks, live cell imaging, and real water sample analysis, supported by theoretical DFT studies.
研究不足
The study is limited to specific chemosensors L1 and L2; other metal ions or conditions may not be covered. Reversibility requires excess EDTA, and practical applications in diverse environments need further validation. Cell imaging was only tested in HeLa cells.
1：Experimental Design and Method Selection:
The study involved synthesizing chemosensors L1 and L2 via Kabachnik-Fields reaction, and evaluating their interaction with Hg2+ ions using UV-Vis and fluorescence spectroscopy, NMR, IR, ESI-MS, and DFT calculations. Reversibility was tested with EDTA.
2：Sample Selection and Data Sources:
Metal ion chloride salts (e.g., Hg2+, Cu2+, etc.) were used as analytes. Real water samples included tap water, river water, and commercial bottled water. HeLa cells were used for cell imaging.
3：List of Experimental Equipment and Materials:
Instruments included Bruker Avance spectrometer for NMR, LCQ Fleet mass spectrometer for ESI-MS, FT-IR spectrometer, Agilent UV-8453 spectrometer for UV-Vis, JASCO FP-6300 fluorescence spectrophotometer, and Gaussian 09 for DFT. Chemicals were from Sigma-Aldrich and Merck.
4：Experimental Procedures and Operational Workflow:
Stock solutions of sensors and metal ions were prepared. Titration studies involved adding Hg2+ ions incrementally and monitoring spectral changes. Competitive studies, reversibility tests with EDTA, cell culture, and real sample analysis were conducted.
5：Data Analysis Methods:
Association constants and detection limits were calculated using Benesi-Hildebrand equation and LOD = K × SD/S. DFT optimized geometries using B3LYP/LANL2DZ basis set.
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