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Evaluation of the Bactericidal Activity of a Hyaluronic Acid-Vehicled Clarithromycin Antibiotic Mixture by Confocal Laser Scanning Microscopy
摘要： Confocal laser scanning microscopy (CLSM) was used to evaluate the antibacterial effect and depth of action of a novel clarithromycin-containing triple antibiotic mixture, which was proposed for root canal disinfection in dental pulp regeneration. A previous study reported that this mixture had no tooth discoloration effects in vitro. After infection with Enterococcus faecalis for 3 weeks, the dentinal tubules in the cylindrical root specimens were exposed to different antibiotic mixtures: ciprofloxacin, metronidazole and minocycline (3-MIX); ciprofloxacin, metronidazole and clarithromycin (3-MIXC) and ciprofloxacin and metronidazole (2-MIX). Each antibiotic formulation was mixed with macrogol (MG) or hyaluronic acid (HA) vehicles. CLSM and viability staining were used to quantitatively analyze the mean depth of the antibacterial effect and the proportions of dead and live bacteria inside the dentinal tubules. The 3-MIX and 3-MIXC demonstrated a similar depth of action. The mean proportion of dead bacteria was similar in the 3-MIX and 3-MIXC groups, and both were statistically higher than that of 2-MIX (p = 0.014). Each antibiotic mixture showed a higher bactericidal efficacy if conveyed with HA, compared to MG (3-MIX, p = 0.019; 3-MIXC, p = 0.013 and 2-MIX, p = 0.0125). The depth of action and the antibacterial efficacy of 3-MIXC seemed comparable with 3-MIX.
关键词： triple antibiotic paste,confocal laser scanning microscopy,double antibiotic paste,regenerative endodontic procedures,hyaluronic acid
更新于2025-09-19 17:13:59
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The fabrication of a biomass carbon quantum dot-Bi <sub/>2</sub> WO <sub/>6</sub> hybrid photocatalyst with high performance for antibiotic degradation
摘要： A novel biomass carbon quantum dots@Bi2WO6 (BC-QDs@Bi2WO6) photocatalyst with uniform dispersivity was prepared via a dialysis-assisted hydrothermal process. Multiple techniques including HR-TEM, XPS, UV-Vis, PL, photocurrent analysis, and electrochemical impedance spectroscopy were employed to investigate the morphology and structures as well as the optical and electronic properties of the as-prepared samples. The photocatalytic activity of BC-QDs@Bi2WO6 was assessed by the degradation of antibiotics (tetracycline, ciprofloxacin, and gatifloxacin). The improved photocatalytic activity was attributed to the quantum effect of BC-QDs, which acted as the light harvesting center, electron reservoir, and reaction center for degrading the pollutants. Furthermore, a plausible mechanism for photocatalysis was put forward according to the ESR and quenching experiments. The present work can have significant advantages in the construction of biomass carbon-based photocatalysts in pollutant degradation and improved light utilization.
关键词： Bi2WO6,dialysis-assisted hydrothermal process,photocatalyst,biomass carbon quantum dots,antibiotic degradation
更新于2025-09-16 10:30:52
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Recycling Oxacillin Residues from Environmental Waste into Graphene Quantum Dots
摘要： Of great concern are the residual antibiotics from dirt that can be found in farm soil and wastewater. This kind of emerging pollutant into engineered nanomaterials is riveting. This work proposes the elimination and transformation of a beta-lactam antibiotic, oxacillin, from environmental waste to graphene quantum dots (GQDs). Two protocols were followed in which the use of ethylenediamine (EDA) in the transformation leads to GQDs with excellent optical properties. Therefore, two types of GQDs were synthesized in a Te?on-lined stainless autoclave by a thermal procedure using oxacillin in the absence and presence of EDA. The ensuing e-GQDs from oxacillin and EDA display a stronger ?uorescence emission in comparison to those synthesized without EDA (o-GQDs). The combination of Kaiser test analyses, infrared (IR) and Raman measurements revealed the presence of oxygen-containing groups and primary amines at the edges of the graphitic nanolayer for e-GQDs. This straightforward strategy brings hope and opens a new interest in waste recycling by means of extracting residual contaminants from the environment for their further transformation into adequate non-toxic graphitic nanomaterials with potential applications.
关键词： residues transformation,photoluminescence,antibiotic,removal
更新于2025-09-16 10:30:52
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Optically-controlled supramolecular self-assembly of an antibiotic for antibacterial regulation
摘要： We report a supramolecular photo-responsive antibiotic (azobenzene-norfloxacin/aCD). This supramolecule exhibited a higher ‘‘on–off’’ ratio of antibacterial ability than azobenzene-norfloxacin alone under UV irradiation. It offers an approach to efficiently regulate the activity of antibiotics by combining the supramolecular and light-regulating strategies together.
关键词： photo-responsive,antibiotic,antibacterial regulation,supramolecular,azobenzene-norfloxacin
更新于2025-09-12 10:27:22
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Mobile Based in Situ Detection of Live/Dead and Antibiotic Resistant Bacteria by Silver Nanorods Array Sensor Fabricated by Glancing Angle Deposition
摘要： The rapid in-situ detection of viability of bacteria is essential for human health and environmental care. It has become one of the biggest needs in biological and medical sciences to prevent infections and diseases, which usually occur in hospitals and field clinics. Nowadays, antibiotic resistance (ABR) has been grown as one of the world’s acutest public health problems, which requires a quick and efficient solution. Here, we demonstrate an easy, sensitive, user-friendly, portable, cost effective and time saving approach for detection of live, dead and drug resistant bacteria. Most of the organisms are found to produce H2S gas by their metabolism system. The endogenous H2S evolution was targeted to differentiate between live and dead as well as ABR bacteria. The silver nanorods (AgNRs) arrays sensors were fabricated by glancing angle deposition technique. The colorimetric and water wettability (contact angle) features of as-synthesized AgNRs were found to be highly sensitive and selective for hydrogen sulfide (H2S) gas. E.coli, P. aeruginosa, B. subtilis and S. aureus were used as the model organisms for this study. A drastic visible change in color as well as wetting properties of AgNRs array was observed. To make it easy, a user friendly and field deployable android based mobile app ‘Colorimetric Detector’ was developed. This dual mode detection is facile, inexpensive and can be easily scaled-up in the field of disease diagnosis.
关键词： mobile app,colorimetric detection,silver nanorods,antibiotic resistance,bacteria detection,H2S gas
更新于2025-09-11 14:15:04
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A system composed of vanadium(IV) disulfide quantum dots and molybdenum(IV) disulfide nanosheets for use in an aptamer-based fluorometric tetracycline assay
摘要： A system composed of vanadium(IV) disulfide quantum dots (VS2 QDs) and molybdenum(IV) disulfide (MoS2) nanosheets for use in an aptamer-based fluorometric tetracycline assay was developed. The tetracycline (TET) aptamer was first immobilzed on the VS2 QDs with a typical size of 3 nm. The blue fluorescence of the VS2 QDs (labeled with aptamer) with emission maxima at 448 nm (under excitation at 360 nm) was subsequently quenched by MoS2 nanosheets. If TET is recognized by the aptamer, the VS2 QDs drift away from the basal plane of the MoS2 nanosheets. This generated “turn-on” fluorescence of the VS2 QDs. AVS2 QD/MoS2 nanosheet-based fluorometric TET aptasensor was thus constructed. Selective and sensitive TET bioanalysis was realized in a linear range of 1 to 250 ng mL?1. The detection limit was 0.06 ng mL?1. Its applicability of determination of TET in milk samples has been demonstrated.
关键词： MoS2,Aptamer,VS2 quantum dots,Fluorometric assay,Antibiotic,Turn-on fluorescence
更新于2025-09-11 14:15:04
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Assessment of thin-film photocatalysis inactivation of different bacterial indicators and effect on their antibiotic resistance profile
摘要： The presence of bacterial pathogens in water bodies, alongside their growing antibiotic resistance, endanger access to freshwater sources and necessitate their successful inactivation with a proper disinfection technology. In the present study, a parallel plate reactor (PPL) with immobilized photocatalyst was used as a disinfection system for the inactivation of two bacterial indicators (Escherichia coli and Enterococcus faecalis) in aqueous samples. Experiments were carried out at parallel plate reactor configuration (PPL) operated in recycling batch mode. Titanium Tetraisopropoxide (TTIP) based thin-film coated photocatalyst surfaces were used and assessed operations parameters were; pH, initial bacteria concentration, source and type of bacteria. The effect of the photocatalytic process on antibiotic resistance profile of target bacteria was also investigated as it may serve as a pioneering step in the field of well-established and modern disinfection method development, without causing proliferation of antibiotic resistance. The observed courses of bacterial inactivation and the final disinfection rates point out diversity in the level of interaction between different type/source of bacteria and photocatalyst of concern. According to the results, a stationary phase of bacteria inactivation proceeded with a rapid and efficient one for the case of E. faecalis (99% removal after 180 min and 99.9% removal after 240 min), while the trend for E. coli is more likely to be described as extended along the process time. Among the tested antibiotics, E. coli Minimum inhibition concentration (MIC) values for beta-lactam, macrolide and aminoglycoside groups were considerably altered (namely Ampicillin, Cefaclor, Clarithromycin - Erythromycin and Amikacin, respectively). PC oxidation was approved to be efficient on bacterial inactivation and trigger alterations on resistance behaviour of E.coli and Enterococcus sp. strains.
关键词： Photocatalysis,Parallel Plate Reactor,Minimum inhibitory concentration (MIC),E. coli,Antibiotic resistance,Inactivation,Thin-Film,E. faecalis
更新于2025-09-10 09:29:36
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Low-blinking SERS substrate for switchable detection of kanamycin
摘要： Metallic plasmonic nanoparticles generate surface-enhanced Raman Scattering (SERS) for wide sensing applications of biomolecules, gas and environmental contaminants. However, the metallic materials cause high thermal radiation during laser excitation in the opto-microfluidic systems and give rise to temporal intensity fluctuations, which is called blinking effects in SERS spectra. This work developed nanocomposite consisting of graphene oxide (GO) and gold nanoparticles (AuNPs) with high thermal stability and low thermal radiation, which provided plasmonic resonance while reduced blinking effect for SERS-based sensing in the microfluidic detection system. The system was applied to detect kanamycin in drinking water, orange juice, and milk by using Texas Red-dye conjugated Beacon aptamer, where the aptamer was designed to be specific and switchable in kanamycin recognition. The sensor was reusable, and its limit of detection was 0.75 nM in a linear range of 1 nM to 100 nM. The microfluidic SERS detection system can be potentially used for sensitive and reliable assays of small molecules.
关键词： Graphene oxide,Gold nanoparticles,SERS,Blinking effects,Antibiotic sensor
更新于2025-09-10 09:29:36
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Smartphone based dual mode in situ detection of viability of bacteria using Ag nanorods array
摘要： The in-situ and rapid detection of live and dead bacteria is essential for human and environmental care. It has become one of the biggest needs in the biological and medical sciences to prevent infectious diseases, which usually occur in hospitals and field clinics. In the current scenario, antibiotic resistance is one of the severe public health problems, which requires a quick and efficient solution. Here, we report a facile sensitive, portable, user-friendly, cost-effective and time saving approach for detection of live, dead and drug-resistant bacteria. The endogenous H2S evolution was targeted to differentiate between live and dead as well as antibiotic resistant bacteria. The silver nanorods (AgNRs) arrays sensors were fabricated by glancing angle deposition technique. The colorimetric and water wettability features of as-synthesized AgNRs are found to be highly sensitive and selective for H2S. E. coli. P. aeruginosa, B. subtilis and S. aureus were used as a model organism in this study. All the bacteria were found to produce H2S by their metabolism process. In order to detect the antibiotic resistant E. coli were grown in the presence of different concentration of ampicillin in Luria broth. A drastic visible change in color as well as wetting of AgNRs array was observed. To make the technique easy, a user-friendly and field deployable mobile app ‘Colorimetric Detector’ was developed. This technique takes only 4-6 hours whereas the conventional methods need around 24 hours for the same. This dual mode facile and, inexpensive method can be easily scaled up in the field of diagnostics.
关键词： live and dead bacteria,hydrogen sulfide,glancing angle deposition,Antibiotic resistance,silver nanorods
更新于2025-09-10 09:29:36
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Pattern-Based Interpretation Criteria for 18F-Fludeoxyglucose Positron Emission Tomography/Computed Tomography in the Assessment of Pyogenic Spine Infection
摘要： 18F-?udeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) is the procedure of choice for investigation of pyogenic spine infection (PSI) when other imaging fails to provide a de?nitive diagnosis, and for assessment of treatment response when patients are not responding as expected to antibiotic therapy [1]. Interpretation criteria for 18F-FDG PET/CT in the initial diagnosis of spine infection have previously relied on the intensity of the 18F-FDG activity. Sites with activity greater than in normal marrow are generally considered actively infected. Results from different series are dif?cult to compare because of different imaging techniques and inclusion criteria. PET-only systems (without the bene?t of a localization CT) were used prior to the availability of hybrid PET/CT. Patient populations were heterogeneous and included spine and peripheral osteomyelitis cases, infection with pyogenic organisms and tuberculosis, and non-operated and postoperative patients with and without hardware. Despite these shortcomings, a review of the early literature [2] and more recent series [3e6] have all reported high sensitivity and speci?city in the diagnosis of spine infection. The localization of 18F-FDG is nonspeci?c and occurs with infection but also with non-septic in?ammation, fracture, or neoplasm. The references quoted above have consisted of random cases, or prospective series of cases, of suspected spine infection. The prevalence of 18F-FDG-avid non-septic conditions in these series is likely low, explaining the relatively high speci?cities reported. False-positive 18F-FDG PET/CT using intensity-based criteria may be more common in problem cases done for initial diagnosis because imaging other than 18F-FDG PET/CT failed to provide a de?nitive diagnosis. Non-septic activity is commonly seen if 18F-FDG PET/CT is done for assessment of antibiotic treatment response for PSI.
关键词： Antibiotic treatment response,18F-?udeoxyglucose positron emission tomography/computed tomography,Pyogenic spine infection,Discitis,Vertebral osteomyelitis
更新于2025-09-10 09:29:36