- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Mobile Teaching and Learning of Coupled-Line Structures: The multiple-1D coupled-line finite-difference time-domain method.
摘要： Fever is one of the most common symptoms of illness in infants and represents a clinical challenge due to the potential for serious bacterial infection. As delayed treatment for these infections has been correlated with increased morbidity and mortality, broad-spectrum β-lactam antibiotics are often prescribed while waiting for microbiological lab results (1–3 days). However, the spread of antibiotic resistance via the β-lactamase enzyme, which can destroy β-lactam antibiotics, has confounded this paradigm; empiric antibiotic regimens are increasingly unable to cover all potential bacterial pathogens, leaving some infants effectively untreated until the pathogen is characterized. This can lead to lifelong sequela or death. Here, we introduce a fluorescent, microfluidic assay that can characterize β-lactamase derived antibiotic susceptibility in 20 min with a sensitivity suitable for direct human specimens. The protocol is extensible, and the antibiotic spectrum investigated can be feasibly adapted for the pathogens of regional relevance. This new assay fills an important need by providing the clinician with hitherto unavailable point of care information for treatment guidance in an inexpensive and simple diagnostic format.
关键词： beta-lactamase,Antibiotic resistance,point of care,microfluidic
更新于2025-09-23 15:21:01
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Integration of plasmonic heating and ona??chip temperature sensor for nucleic acid amplification assays
摘要： Nucleic acid tests have been widely used for diagnosis of diseases by detecting the relevant genetic markers that are usually amplified using polymerase chain reaction (PCR). This work reports the use of a plasmonic device as an efficient and low-cost PCR thermocycler to facilitate nucleic acid-based diagnosis. The thermoplasmonic device, consisting of a one-dimensional metal grating, exploited the strong light absorption of plasmonic resonance modes to heat up PCR reagents using a near-infrared laser source. The plasmonic device also integrated a thin-film thermocouple on the metal grating to monitor the sample temperature. The plasmonic thermocycler is capable of performing a PCR amplification cycle in approximately 2.5 minutes. We successfully demonstrated the multiplex and real-time PCR amplifications of the antibiotic resistance genes using the genomic DNAs extracted from Acinetobacter baumannii, Klebsiella pneumonia, Escherichia coli, and Campylobacter.
关键词： polymer chain reaction,thermoplasmonics,thermocycler,nucleic acid-based diagnostics,antibiotic resistance detection,Photothermal effect
更新于2025-09-23 15:19:57
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Mobile Based in Situ Detection of Live/Dead and Antibiotic Resistant Bacteria by Silver Nanorods Array Sensor Fabricated by Glancing Angle Deposition
摘要： The rapid in-situ detection of viability of bacteria is essential for human health and environmental care. It has become one of the biggest needs in biological and medical sciences to prevent infections and diseases, which usually occur in hospitals and field clinics. Nowadays, antibiotic resistance (ABR) has been grown as one of the world’s acutest public health problems, which requires a quick and efficient solution. Here, we demonstrate an easy, sensitive, user-friendly, portable, cost effective and time saving approach for detection of live, dead and drug resistant bacteria. Most of the organisms are found to produce H2S gas by their metabolism system. The endogenous H2S evolution was targeted to differentiate between live and dead as well as ABR bacteria. The silver nanorods (AgNRs) arrays sensors were fabricated by glancing angle deposition technique. The colorimetric and water wettability (contact angle) features of as-synthesized AgNRs were found to be highly sensitive and selective for hydrogen sulfide (H2S) gas. E.coli, P. aeruginosa, B. subtilis and S. aureus were used as the model organisms for this study. A drastic visible change in color as well as wetting properties of AgNRs array was observed. To make it easy, a user friendly and field deployable android based mobile app ‘Colorimetric Detector’ was developed. This dual mode detection is facile, inexpensive and can be easily scaled-up in the field of disease diagnosis.
关键词： mobile app,colorimetric detection,silver nanorods,antibiotic resistance,bacteria detection,H2S gas
更新于2025-09-11 14:15:04
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Assessment of thin-film photocatalysis inactivation of different bacterial indicators and effect on their antibiotic resistance profile
摘要： The presence of bacterial pathogens in water bodies, alongside their growing antibiotic resistance, endanger access to freshwater sources and necessitate their successful inactivation with a proper disinfection technology. In the present study, a parallel plate reactor (PPL) with immobilized photocatalyst was used as a disinfection system for the inactivation of two bacterial indicators (Escherichia coli and Enterococcus faecalis) in aqueous samples. Experiments were carried out at parallel plate reactor configuration (PPL) operated in recycling batch mode. Titanium Tetraisopropoxide (TTIP) based thin-film coated photocatalyst surfaces were used and assessed operations parameters were; pH, initial bacteria concentration, source and type of bacteria. The effect of the photocatalytic process on antibiotic resistance profile of target bacteria was also investigated as it may serve as a pioneering step in the field of well-established and modern disinfection method development, without causing proliferation of antibiotic resistance. The observed courses of bacterial inactivation and the final disinfection rates point out diversity in the level of interaction between different type/source of bacteria and photocatalyst of concern. According to the results, a stationary phase of bacteria inactivation proceeded with a rapid and efficient one for the case of E. faecalis (99% removal after 180 min and 99.9% removal after 240 min), while the trend for E. coli is more likely to be described as extended along the process time. Among the tested antibiotics, E. coli Minimum inhibition concentration (MIC) values for beta-lactam, macrolide and aminoglycoside groups were considerably altered (namely Ampicillin, Cefaclor, Clarithromycin - Erythromycin and Amikacin, respectively). PC oxidation was approved to be efficient on bacterial inactivation and trigger alterations on resistance behaviour of E.coli and Enterococcus sp. strains.
关键词： Photocatalysis,Parallel Plate Reactor,Minimum inhibitory concentration (MIC),E. coli,Antibiotic resistance,Inactivation,Thin-Film,E. faecalis
更新于2025-09-10 09:29:36
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Smartphone based dual mode in situ detection of viability of bacteria using Ag nanorods array
摘要： The in-situ and rapid detection of live and dead bacteria is essential for human and environmental care. It has become one of the biggest needs in the biological and medical sciences to prevent infectious diseases, which usually occur in hospitals and field clinics. In the current scenario, antibiotic resistance is one of the severe public health problems, which requires a quick and efficient solution. Here, we report a facile sensitive, portable, user-friendly, cost-effective and time saving approach for detection of live, dead and drug-resistant bacteria. The endogenous H2S evolution was targeted to differentiate between live and dead as well as antibiotic resistant bacteria. The silver nanorods (AgNRs) arrays sensors were fabricated by glancing angle deposition technique. The colorimetric and water wettability features of as-synthesized AgNRs are found to be highly sensitive and selective for H2S. E. coli. P. aeruginosa, B. subtilis and S. aureus were used as a model organism in this study. All the bacteria were found to produce H2S by their metabolism process. In order to detect the antibiotic resistant E. coli were grown in the presence of different concentration of ampicillin in Luria broth. A drastic visible change in color as well as wetting of AgNRs array was observed. To make the technique easy, a user-friendly and field deployable mobile app ‘Colorimetric Detector’ was developed. This technique takes only 4-6 hours whereas the conventional methods need around 24 hours for the same. This dual mode facile and, inexpensive method can be easily scaled up in the field of diagnostics.
关键词： live and dead bacteria,hydrogen sulfide,glancing angle deposition,Antibiotic resistance,silver nanorods
更新于2025-09-10 09:29:36