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Nitrogen-Doped Titanium Dioxide Thin Films Formation on the Surface of PLLA Electrospun Microfibers Scaffold by Reactive Magnetron Sputtering Method
摘要： Nitrogen-doped thin titanium dioxide films formed by the reactive magnetron sputtering method on the surface of PLLA electrospun microfibers scaffold were investigated. It was shown that the chemical composition of the films is shifting from titanium dioxide (TiO2) composites saturated with C–NH, C=N, N–C=N and HN–C=O compounds to solid solutions of titanium oxides (TixOy) and titanium oxynitrides (TiOxNy) with the increased time of the treatment. An empirical model describing changes in the chemical composition of the surface due to the treatment was proposed. It was shown that the modification of the PLLA microfibers scaffolds surface improves cell-scaffold and cell–cell interactions with the highest number of viable adherent cells observed on the scaffold treated for 4 min.
关键词： Scaffolds,Reactive magnetron sputtering,Thin films,Biocompatibility
更新于2025-11-21 11:24:58
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Biocompatible photoluminescent silk fibers with stability and durability
摘要： Exploring photoluminescent silk fibers, possessing biocompatibility as well as stable and durable fluorescent properties, is a requirement for the development of novel photoluminescent biomaterials. Herein, we fabricate photoluminescent silk fibers, TPCA@SF, via modifying an organic fluorescent molecule (5-oxo-3,5-dihydro-2H-thiazolo [3,2-a] pyridine-7-carboxylic acid, TPCA) onto silk fibers, along with using quaternary ammonium salt didodecyldimethylammonium bromide (DDAB) as color fixing agent. The hydrogen bonds and electrostatic association among silk fibers, TPCA and DDAB ensure the stable modification. The facile and green fabrication process is achieved in water under mild conditions without using any toxic substances. The TPCA@SF manifests the combining features of high quantum yield, fluorescence water-fastness, anti-photobleaching, good mechanical property and biocompatibility. The strategy holds great potential for exploring various biocompatible photoluminescent substances with stability and durability.
关键词： wash-durability,biocompatibility,photoluminescence,silk fibers,anti-photobleaching
更新于2025-11-21 11:24:58
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In vitro bioactivity and biocompatibility of femtosecond laser-modified Ti6Al4V alloy
摘要： The present work investigates bioactivity and biocompatibility of femtosecond (fs) laser surface-modified Ti6Al4V alloy (Ti-alloy). Self-aligned conical surface features were generated on Ti-alloy when laser irradiated employing a Ti:sapphire pulsed fs laser of wavelength 800 nm. Modification of surface chemical composition resulting from fs-laser irradiation of Ti-alloy was examined using Grazing incidence X-ray diffraction (GIXRD) technique and micro-Raman spectroscopy. Sub-oxide phase of titanium was detected on Ti-alloy surface post-fs-laser irradiation leading to increased oxygen vacancies on sample surface. For in vitro bioactivity tests, untreated and fs-laser-treated samples were immersed in simulated body fluid for 2 weeks. Evidence of hydroxyapatite deposition on both untreated Ti-alloy, as well as, fs-laser-treated Ti-alloy surfaces after in vitro tests were provided by scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDX), GIXRD, Fourier transform infrared spectroscopy (FTIR), and micro-Raman spectroscopy. Superior growth of HAP was observed on fs-laser-modified Ti-alloy surface in comparison with untreated surface. Biocompatibility of the laser-treated Ti-alloy was investigated by studying anchoring and growth of human osteosarcoma cell line (U2OS) on it. Using MTT assay technique in vitro cell viability and growth potential in the presence of untreated and laser-treated Ti-alloy samples were assessed. MTT test results demonstrated that, neither cell viability, nor growth were affected in the presence of either the untreated or laser-treated sample surfaces. In addition, in comparison with the untreated Ti-alloy surface, the fs-laser-treated Ti-alloy surface showed more efficient cellular attachment when examined under confocal microscope.
关键词： Biocompatibility,Hydroxyapatite,Surface modification,Ti6Al4V alloy,Femtosecond laser,Bioactivity
更新于2025-11-21 11:08:12
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TiO2 Rutile phase formed interlayers by sintering mono phasic bioceramics for Biomedical applications
摘要： The formation of rutile TiO2 phase in nanoHydroxyapatite(nanoHAP) coated titanium metal (nanHAP- TiO2-Ti) is reported. This work is mainly emphasized in order to enhance the corrosion protection performance, osseointegration and biocompatibility. The optimized potential of nanoHAP coated titanium using electrophoretic deposition (EPD) was found to be 80 V and 3 minutes. The coated samples were sintered in air at various sintering temperatures from 600 to 900°C for 1 hour. The crystallinity, vibrational states, surface morphology and surface topography were characterized by XRD, Raman, FESEM with EDAX and AFM studies respectively. In the present work, investigations on the corrosion behavior of sintered sample in Ringer’s solution by means of electrochemical studies using Open Circuit Potential (OCP), Electrochemical Impedance spectroscopic(EIS) and anodic polarization studies were carried out. The electrochemical studies exhibited an enhanced corrosion resistant behaviour towards nanoHAP coated titanium sintered at 800oC with complete rutile TiO2 phase formation. The in vitro cytotoxicity studies of the coated materials were carried out by MTT assay and cell attachment with osteoblast cells. These studies revealed an enhanced cell attachment and proliferation on the composite layer compared to uncoated metal, which controlled the release of metal ions into the biological system.
关键词： Biocompatibility,nanHAP- TiO2-Ti,EPD,EIS,Sintering
更新于2025-11-21 11:08:12
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Cell viability assessments of green synthesized water-soluble AgInS <sub/>2</sub> /ZnS core/shell quantum dots against different cancer cell lines
摘要： Chalcopyrite quantum dots (QDs) have emerged as a safe alternative to cadmium-based QDs for bio-applications. However, the research on AgInS2 chalcopyrite QDs has not been widely explored in terms of their toxicity. Herein, we report a synthesis of biocompatible AgInS2/ZnS QDs via a greener approach. The emission intensity of the as-synthesized AgInS2 core QDs was enhanced 2-fold after the ZnS shell growth. X-ray diffraction revealed the tetragonal crystal structure of QDs, and high-resolution transmission electron microscope images show that the QDs are spherical in shape and crystalline in nature. Cell viability assays conducted on different cell lines, such as HeLa, A549, and BHK-21 cells, indicated that AgInS2/ZnS QDs are least toxic at a QD concentration range of 100 lg/mL. The ?uorescent microscope analysis of A549 cells incubated with AgInS2/ZnS QDs shows that the QDs were accumulated in the cell membranes. The as-synthesized AgInS2/ZnS QDs are less toxic and eco-friendly, and can be used for biolabeling.
关键词： cell viability,AgInS2/ZnS,biolabeling,Chalcopyrite quantum dots,biocompatibility
更新于2025-11-19 16:46:39
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Biocompatible pure ZnO nanoparticles-3D bacterial cellulose biointerfaces with antibacterial properties
摘要： In this paper, we present for the first time the obtaining and characterization of new antibacterial and biocompatible nano-ZnO–bacterial cellulose (BC) material with controlled interfaces for studying in vitro microorganisms (Escherichia Coli (ATCC 8737), B. subtilis Spizizenii Nakamura (ATCC 6633), Candida albicans (ATCC10231)) and mammalian cells (human dermal fibroblast cells) response. The use of BC based material with controlled characteristics in terms of quantity and distribution of ZnO onto BC membrane (with 2D and 3D fibers arrangement) is directly correlated with the surface chemical and topographical properties, the method of preparation, and also with the type of cells implied for the specific application within the bioengineering fields. In our study, the uniform distribution and the control on the quantity of ZnO nanoparticles onto 3D BC were obtained using matrix assisted pulsed laser evaporation (MAPLE) method. The influence on particle distribution onto 3D bio cellulose were investigated based on two types of solvents (water and chloroform) involved in target preparation within MAPLE deposition. The attachment of the nanoparticles to the bacterial cellulose surface and fibrils was demonstrated by SEM and FT-IR studies. The BC-ZnO showed both resistance to bacteria-sticking and non-cytotoxic effect on the human dermal fibroblasts cells at a mass distribution onto surface of 1.68 mg ZnO NPS/mm2. These results represent a good premise in terms of tailoring BC substrates with ZnO particles that could determine or enhance both the biocompatibility and antibacterial properties of BC-composite materials.
关键词： Antibacterial effect,Zinc oxide nanoparticles,MAPLE deposition technique,Bacterial cellulose,Biocompatibility
更新于2025-09-23 15:23:52
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Near infrared light-responsive heat-emitting hemoglobin hydrogels for photothermal cancer therapy
摘要： Photothermal therapy (PTT) is an effective means of treating tumors because tumor cells are sensitive to heat. Gold and carbon nanoparticles are used as efficient PTT materials. However, development of a non-toxic biodegradable PTT agent remains a challenge. Here, we developed a hemoglobin (Hb) hydrogel that exhibited excellent PTT effects in vitro and in vivo. Unlike conventional PTT agents, which are toxic and do not decompose completely in the body, the Hb hydrogel was manufactured using only two components: (i) Hb, a natural substance derived from the human body, and (ii) PEG, an FDA-approved polymer. The gelation time of the Hb hydrogels could be controlled by changing the Hb concentration. Because Hb is present at a high concentration (150 mg/ml) in the body, the Hb hydrogel decomposed and was eliminated in vivo without toxicity. The Hb hydrogel showed an excellent PTT effect in response to 808 nm near-infrared (NIR) laser irradiation and had excellent anticancer effects against A549 lung cancer cells both in vitro and in vivo. Blood hematology and blood biochemical assay results from an animal model treated with Hb hydrogel were similar to those of the control group. Importantly, toxicity was not observed based on H&E staining of major organs (heart, liver, spleen, kidneys and lung). Tumors of A549 cell-xenografted mice treated with Hb hydrogel and 808 nm NIR laser irradiation were significantly smaller than those of the control group (23.1 mm3 versus 746.5 mm3, respectively). This is a first report of a biocompatible photothermal hydrogel based on hemoglobin, and our overall results suggest that Hb hydrogels are commercially-promising PTT systems that have excellent anti-cancer effects.
关键词： Biocompatibility,Quick gelation,Lung cancer,Hemoglobin,Hydrogel,Photothermal therapy
更新于2025-09-23 15:23:52
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Ultrasmall Au-Ag Alloy Nanoparticles: Protein-directed Synthesis, Biocompatibility and X-ray Computed Tomography Imaging ?
摘要： The ultrasmall sizes of nanoparticles have attracted significant attention for potential application in the fields of catalysis and nanomedicine. Herein, we reported on the green preparation and X-ray computed tomography (CT) imaging of ultrasmall bimetallic bovine serum albumin-directed gold-silver (Au-Ag@BSA) nanoparticles (2–4 nm) using BSA as a stabilizing and template-directed agent. Further, the effects of synthesis condition were systematically explored to prepare products by adjusting the different molar ratios of Au/Ag. The resulting Au-Ag@BSA nanoparticles exhibited the spherical shape, well-dispersed ability, as well as long-term room-temperature stability. The cytotoxicity effects of Au-Ag@BSA nanoparticles on A549 and MCF-7 cells were compared with those of individual Ag nanoparticles, and the results indicated lower cytotoxicity effect by Au-Ag@BSA nanoparticles. Furthermore, the in vivo toxicity of Au-Ag@BSA nanoparticles was investigated in the early-stage zebrafish embryos. The results indicate that there are not any obvious changes of survival and hatching percentages at multiple growth stages (4-120 hpf) even a high level of Au-Ag@BSA nanoparticles (up to 80 mM), revealing the good biocompatibility. Interestingly, a rational design of Au/Ag molar ratio (3:2) surprisingly possessed the enhanced CT performances compared to the Au nanoparticles and iohexol. Accordingly, this study highlights a new prospect in the green preparation of ultrasmall alloy nanomaterials with good biocompatibility and will be of great interest in developing CT contrast agent, catalyst as well as drug delivery carrier.
关键词： green synthesis,Au-Ag@BSA nanoparticles,biocompatibility,zebrafish embryos,enhanced CT imaging
更新于2025-09-23 15:23:52
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An inorganic magnetic fluorescent nanoprobe with favorable biocompatibility for dual-modality bioimaging and drug delivery
摘要： In this work, we present the development of a high water soluble biological nanoprobe through the covalent bonding of β-CD onto the surface of iron oxide?gold nanoclusters (Fe3O4@Au@β-CD). Fe3O4@Au@SiO2 NPs were also prepared for comparison. The maximum emission peak of magnetic fluorescent NPs red-shifted by 30 nm and the lifetime was also elongated to 5.21 μs after surface modified with β-CD. The relaxivities and in vitro magnetic resonance imaging ability of the resultant magnetic fluorescent NPs were also studied, indicating that Fe3O4@Au@β-CD NPs have the lowest r2/r1 ratio and could be a potential T2 contrast agent for MR imaging. The MTT assay proved that, Fe3O4@Au@β-CD NPs are of excellent water solubility and biocompatibility. In vitro confocal fluorescence imaging was also performed, manifesting that Fe3O4@Au@β-CD NPs can be selectively uptaken by gastric cancer cells (MGC-803) and exhibit red fluorescence in the cells. The preliminary drug loading and releasing measurements demonstrate that it can also act as targeted drug delivery nanosystem. All these experimental results indicate that, Fe3O4@Au@β-CD NPs hold great application perspective in the diagnosis and therapy of gastric cancer cells as biological nanoprobes.
关键词： Multifunctional,Biocompatibility,Magnetic fluorescent nanocomposites,Drug delivery,Dual-modality imaging
更新于2025-09-23 15:23:52
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Bright red-emitting highly reliable styryl probe with large stokes shift for visualizing mitochondria in live cells under wash-free conditions
摘要： Bright red-emitting pyridinium cyanine based styryl probe 2 is synthesized in good yields. Probe 2 demonstrated a large Stokes’ shift (Δλ ≈ 128 nm, 4227 cm-1 in DCM) and excellent fluorescent quantum yield (?fl ≈ 0.2 - 0.7) due to strong Intra-molecular charge transfer (ICT). Probe 2 found to exhibit exceptional selectivity for cellular mitochondria in both normal (COS-7) and cancer (A549) cell lines. Probe 2 is readily applicable as a “wash-free” dye to visualize mitochondria as it does not require post-staining washing prior to imaging. Styryl probe 2 also showed an excellent biocompatibility as the calculated LC50 (lethal concentration, 50%) value was > 20 μM. Probe 2 emission did not show any interferences from anionic species or other biological molecules. Probe 2 is readily excitable (λex ~460 and λem ~618 nm) with the available laser (454 nm) in commercial microscopes and thus it can be a useful probe for mitochondrial tracking in live cells.
关键词： Wash-free application,Biocompatibility,Intra-molecular charge transfer (ICT),Styryl dye,Large Stokes shift,Mitochondria selectivity
更新于2025-09-23 15:23:52