- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Facile fabrication of cross-linked fluorescent organic nanoparticles with aggregation-induced emission characteristic via the thiol-ene click reaction and their potential for biological imaging
摘要： Over the past several years, the biomedical applications of fluorescent organic nanoparticles (FONs) with aggregation-induced emission (AIE) feature have been extensively explored because the AIE-active FONs could effectively overcome the aggregation caused quenching (ACQ) effect of FONs based on conventional organic dyes. The development of novel methods for synthesis of AIE-active FONs plays a centre role for their biomedical applications. In this work, we reported a facile one-step thiol-ene click reaction for fabrication of AIE-active FONs through conjugation of acrylated PEG and AIE-active tetraphenylethylene (TPE) with two ene bonds using pentaerythritol tetra(3-mercaptopropionate) as the linkage. The successful synthesis of TPE containing AIE-active copolymers was evidenced by various characterization techniques. The particle size and fluorescence properties of the resultant TPE-S-PEG copolymers were evaluated by transmission electronic microscopy and fluorescence spectroscopy. Moreover, the cell viability and cell uptake behavior was also examined to evaluate their potential for biological imaging. We demonstrated that the cross-linked TPE-S-PEG show small size, high water dispersibility, low cytotoxicity and strong fluorescence for tracing. All of these advantages endow the TPE-S-PEG FONs great potential for biological imaging applications. Furthermore, this novel click reaction can take place under mild experimental conditions with high efficiency. It could be also further expanded for preparation of multifunctional AIE-active materials due to the universality of the thiol-ene click reaction and good precursor applicapability. Taken together, we have developed a novel and effective thiol-ene click reaction to fabricate the cross-linked AIE-active FONs, which display excellent physicochemical and biological properties and are promising for biomedical applications.
关键词： thiol-ene click reaction,Aggregation-induced emission,biomedical applications,fluorescent organic nanoparticles,cross-linked FONs
更新于2025-09-23 15:23:52
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Gamma irradiated poly (methyl methacrylate)-reduced graphene oxide composite thin films for multifunctional applications
摘要： Poly (methyl methacrylate) (PMMA)-Reduced Graphene Oxide (rGO) (PrGO) composite films were fabricated by solvent evaporation technique and exposed to gamma radiation at different dosages viz. 25 kGy, 50 kGy and 100 kGy. The XRD analysis revealed the phases of PMMA and rGO and further confirmed the semi-crystalline nature of PMMA. The irradiation also decreased the peak intensities of the functional groups of PMMA and rGO. At 50 kGy irradiation, lamellar structures were formed on the surface of the films (50 kGy) due to the thermal fluctuations whereas, at higher dosage (100 kGy), pores were formed. The surface roughness and contact angle were enhanced on 50 kGy sample. The drug impregnated PrGO50 and PrGO100 samples showed sustained and burst release of drug respectively and in addition exhibited a better zone of inhibition against E. coli bacteria. All the samples were hemocompatible in nature. Fibroblast proliferation was enhanced with no cytotoxic effect on 50 kGy samples. Hence, the gamma irradiated samples could be an excellent candidate for biosensing and biomedical applications.
关键词： Biomedical Applications,Polymer,Bioactive,Reduced-graphene oxide composites
更新于2025-09-23 15:23:52
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Fluorescent Nanodiamonds || Cell Labeling and Fluorescence Imaging
摘要： Nanocarbons are a family consisting of fullerenes, carbon nanotubes, graphenes, nanodiamonds, and other variations. Fluorescent nanodiamonds (FNDs) joined the family in 2005. While receiving less attention than other members of the family, FNDs are gaining popularity as a novel nanoparticle platform for biomedical applications in recent years. An ideal biomedical nanoparticle platform should possess three functionalities: targeting, imaging, and therapeutic. Of course, the nanoparticles must be endowed with high biocompatibility and low cytotoxicity. As evident from all the discussions so far in this book, surface‐functionalized FND clearly meets all these requirements. Applying the platform to biomedical studies is expected to enhance our understanding of the pathophysiological basis of disease, open up more sophisticated diagnostic opportunities, and lead to improved therapies and treatments.
关键词： Imaging,Targeting,Fluorescent nanodiamonds,Nanocarbons,Therapeutic,Biomedical applications
更新于2025-09-23 15:21:01
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Atomic Force Microscopy in Molecular and Cell Biology || AFM Imaging-Force Spectroscopy Combination for Molecular Recognition at the Single-Cell Level
摘要： Molecular recognition at the single-cell level is an increasingly important issue in Biomedical Sciences. With atomic force microscopy, cell surface receptors may be recognized through the interaction with their ligands, inclusively for the identification of cell-cell adhesion proteins. The spatial location of a specific interaction can be determined by adhesion force mapping, which combines topographic images with local force spectroscopy measurements. Another valuable possibility is to simultaneously record topographic and recognition images (TREC imaging) of cells, enabling the mapping of specific binding events on cells in real time. This review is focused on recent developments on these molecular recognition approaches, presenting examples of different biological and biomedical applications.
关键词： Molecular recognition,TREC imaging,Atomic force microscopy,Adhesion force mapping,Biomedical applications
更新于2025-09-23 15:21:01
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Evolution of nanocrystal size distribution in porous silicon nanoparticles during storage in aqueous media: X-ray diffraction analysis
摘要： X-ray diffraction studies of electrochemically prepared mesoporous and microporous silicon particles were carried out to monitor their dissolution in aqueous media. The dissolution process was found to result in either decreasing or an increasing of the mean size of silicon nanocrystallites in mesoporous and microporous samples, respectively. The evolution of nanocrystallite size was related by polydispersity of the initial size distribution and it was described by using a model of Bshrinking spheres.^ The proposed approach was used to confirm an effect of biopolymer surrounding of silicon nanocrystallites on their stability in aqueous medium. The obtained results and developed model can be useful for potential biomedical applications of porous silicon.
关键词： Biomedical applications,Oxidation,Nanoparticles,Diffraction,Silicon,X-ray
更新于2025-09-19 17:15:36
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Biomedical applications of mid-infrared quantum cascade lasers – a review
摘要： Mid-infrared spectroscopy has been applied to research in biology and medicine for more than 20 years and conceivable applications have been identified. More recently, these applications have been shown to benefit from the use of quantum cascade lasers due to their specific properties, namely high spectral power density, small beam parameter product, narrow emission spectrum and, if needed, tuning capabilities. This review provides an overview of the achievements and illustrates some applications which benefit from the key characteristics of quantum cascade laser-based mid-infrared spectroscopy using examples such as breath analysis, the investigation of serum, non-invasive glucose monitoring in bulk tissue and the combination of spectroscopy and microscopy of tissue thin sections for rapid histopathology.
关键词： quantum cascade lasers,glucose monitoring,mid-infrared spectroscopy,histopathology,breath analysis,biomedical applications
更新于2025-09-19 17:15:36
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Surface grafting of fluorescent polymers on halloysite nanotubes through metal-free light-induced controlled polymerization: Preparation, characterization and biological imaging
摘要： Halloysite nanotubes (HNTs) are a kind of aluminosilicate clay with a unique hollow tubular structure that has been intensively explored for various applications especially in biomedical fields owing to their excellent biocompatibility, biodegrading potential and low cost. Surface modification of HNTs with functional polymers will greatly improve their properties and endow new functions for biomedical applications. In this work, a light-induced reversible addition-fragmentation chain transfer (RAFT) polymerization was introduced to successfully prepare HNTs based fluorescent HNTs/poly(PEGMA-Fl) composites in the presence of oxygen using diacrylate-fluorescein and poly (ethylene glycol) methyl ether methacrylate (PEGMA) as the monomers. Without other catalysts, heating, and deoxygenation procedure, the polymerization process can take place under mild conditions. Besides, owing to the introduction of fluorescein and PEGMA on the surface of HNTs, the resultant HNTs/poly(PEGMA-Fl) composites display high water dispersibility and stable fluorescence. The results from cell viability examination and confocal laser scanning microscopy also demonstrated that HNTs/poly(PEGMA-Fl) composites could be internalized by L929 cells with bright fluorescence and low cytotoxicity. Taken together, we developed a novel photo-initiated RAFT polymerization method for the fabrication of HNTs based fluorescent polymeric composites with great potential for biomedical applications. More importantly, many other multifunctional HNTs based polymer composites could also be fabricated through a similar strategy owing to good designability of RAFT polymerization.
关键词： fluorescent composites,biomedical applications,Halloysite nanotubes,light-induced polymerization,surface-initiated RAFT polymerization
更新于2025-09-19 17:13:59
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Recent Advances in Biopolymers || Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques
摘要： This chapter provides an overview of recent advances in the field of laser-based synthesis of biopolymer thin films for biomedical applications. The introduction addresses the importance of biopolymer thin films with respect to several applications like tissue engineering, cell instructive environments, and drug delivery systems. The next section is devoted to applications of the fabrication of organic and hybrid organic–inorganic coatings. Matrix-assisted pulsed laser evaporation (MAPLE) and Combinatorial-MAPLE are introduced and compared with other conventional methods of thin films assembling on solid substrates. Advantages and limitations of the methods are pointed out by focusing on the delicate transfer of bio-macromolecules, preservation of properties and on the prospect of combinatorial libraries’ synthesis in a single-step process. The following section provides a brief description of fundamental processes involved in the molecular transfer of delicate materials by MAPLE. Then, the chapter focuses on the laser synthesis of two polysaccharide thin films, namely Dextran doped with iron oxide nanoparticles and Levan, followed by an overview on the MAPLE synthesis of other biopolymers. The chapter ends with summary and perspectives of this fast-expanding research field, and a rich bibliographic database.
关键词： MAPLE and Combinatorial-MAPLE,thin films,biomedical applications,Biopolymers
更新于2025-09-19 17:13:59
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A Multiparametric Evaluation of Quantum Dot Size and Surface-Grafted Peptide Density on Cellular Uptake and Cytotoxicity
摘要： Despite the progress in nanotechnology for biomedical applications, great efforts are still being employed in optimizing nanoparticle (NP) design parameters to improve functionality and minimize bionanotoxicity. In this study, we developed CdSe/CdS/ZnS core/shell/shell quantum dots (QDs) that are compact ligand-coated and surface-functionalized with an HIV-1-derived TAT cell-penetrating peptide (CPP) analog to improve both biocompatibility and cellular uptake. Multiparametric studies were performed in different mammalian and murine cell lines to compare the effects of varying QD size and number of surface CPPs on cellular uptake, viability, generation of reactive oxygen species, mitochondrial health, cell area, and autophagy. Our results showed that the number of cell-associated NPs and their respective toxicity are higher for the larger QDs. Meanwhile, increasing the number of surface CPPs also enhanced cellular uptake and induced cytotoxicity through the generation of mitoROS and autophagy. Thus, here we report the optimal size and surface CPP combinations for improved QD cellular uptake.
关键词： cellular uptake,mitochondrial health,cytotoxicity,biomedical applications,reactive oxygen species,nanotechnology,autophagy,quantum dots
更新于2025-09-19 17:13:59
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MAPbI <sub/>3</sub> Self‐Recrystallization Induced Performance Improvement for Oxygen‐Containing Functional Groups Decorated Carbon Nanotube‐Based Perovskite Solar Cells
摘要： The specific interactions (i.e., molecular recognitions) between receptors at membrane and ligands at the extracellular matrix (ECM) are crucial in various cellular processes [1]. The occurrence of these molecular recognitions as a consequence of ECM remodeling gives rise to specific cell-signaling and intracellular cascades. Therefore the natural receptor(cid:1)ligand interactions are central in physiology and pathological processes. From the point of view of materials science, the specific binding of natural receptors to targeted ligands also shows great promise in the design of biomaterials with advanced affinity [2]. Despite the successful development of a plethora of biomaterials or biomedical devices based on natural receptor(cid:1)ligand interactions in the last decade, natural species like proteins or DNAs still have inherent drawbacks [3,4]. First, the chemical and physical stability and shelf life of natural receptors or antibodies are limited, which restricts their applications in nonphysiological environments. Second, it is expensive, time-consuming, and labor-intensive to isolate and purify them from nature or by biochemical synthesis. Finally, practical requirements in materials science are more extensive than the limited yield, functions, and diversity in currently available molecular recognition in nature. As a result, an advanced material design with more durable and robust receptor-like substitutions is sought [5].
关键词： Bioaffinity,Synthetic Receptors,Affinity Screening,Molecular Imprinting,Biomedical Applications
更新于2025-09-19 17:13:59