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A near-infrared BODIPY-based fluorescent probe for ratiometric and discriminative detection of Hg2+ and Cu2+ ions in living cells
摘要： A near-infrared distyryl boron dipyrromethene-based sensor bearing one bis(1,2,3-triazole)amino receptor has been synthesized. This probe selectively and quickly binds to Hg2+ and Cu2+ ions in CH3CN/H2O (5:1 v/v) and exhibits remarkably blue-shifted absorption and fluorescence bands due to the inhibition of the intramolecular charge transfer process. The fluorescence changes of this probe upon binding to Hg2+ or Cu2+ ion are totally different, undergoing a ratiometric fluorescence enhancement (for Hg2+) or a fluorescence quenching (for Cu2+) mechanism. The corresponding vivid color changes can be easily seen by the naked eye. This probe was further introduced into Hela cells for living cell imaging and found to discriminate Hg2+ and Cu2+ ions through two near-infrared fluorescence emission channels. These overall results indicate that this Click-derived near-infrared BODIPY-based probe is potentially useful for ratiometric and discriminative detection of Hg2+ and Cu2+ ions in solutions and living cells.
关键词： Discriminative detection,Ratiometric,Hg2+ and Cu2+ sensor,Near-infrared,BODIPY
更新于2025-11-21 11:08:12
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A sequential and reversibility fluorescent pentapeptide probe for Cu(II) ions and hydrogen sulfide detections and its application in two different living cells imaging
摘要： In this study, we report a sequential and reversibility fluorescent probe (DP5) based on pentapeptide conjugated with dansyl groups using the solid phase peptide synthesis (SPPS) technology. DP5 showed immediate “turn off” response toward Cu2+ ions at an excitation wavelength of 330 nm with detection limits of 23.5 nM. The 2:1 binding ratio between DP5 and Cu2+ were confirmed using Job's plot method and fluorescence titration study, and DP5-Cu complex was observed with an association constant of 6.76 × 108 M?2. As designed, DP5-Cu complex as a promising analytical probe exhibited highly selective for H2S detection in aqueous solutions. The detection limit for H2S was obtained to be 17.2 nM, and lower than EPA and WHO guidelines. In addition, the reversibility and cyclicity were imparted to the DP5 during the detection of Cu2+ and H2S, and cycle effect is very good. Furthermore, DP5 displayed better biocompatibility and low biotoxicity, and sequential fluorescence “on-off-on” responses of DP5 to Cu2+ and H2S were successfully applied in two different living cells.
关键词： Cu2+ ions,Pentapeptide,Fluorescent probe,Cell imaging,Hydrogen sulfide,Aqueous solutions
更新于2025-11-21 11:08:12
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Phenanthroline-Derivative Functionalized Carbon Dots for Highly Selective and Sensitive Detection of Cu2+ and S2? and Imaging inside Live Cells
摘要： Developing effective methods for the instant detection of Cu2+ and S2? is highly desired in the biological and environmental fields. Herein, a novel fluorescent nanoprobe was elaborately designed and synthesized by grafting a phenanthroline derivative onto the surface of carbon dots (CDs). The obtained functionalized CDs (FCDs) exhibited blue fluorescence (FL) with excellent photostability and possessed a mean diameter around 4 nm. Cu2+ can be selectively captured by the phenanthroline group of FCDs to generate an absorptive complex in situ, leading to obvious quenching of the FCDs’ FL signal through an inner filter effect. Furthermore, the FL of the FCD–Cu2+ can be effectively recovered by S2? anions due to the release of FCDs from the FCD–Cu2+ complex owing to the formation of stable CuS (Ksp = 1.27 × 10?36) between S2? and Cu2+. The detection limits of the FCDs were determined to be 40.1 nM and 88.9 nM for Cu2+ and S2?, respectively. Moreover, this nanoprobe can also be used for the imaging of intracellular Cu2+ and S2?, which shows strong application prospects in the field of biology.
关键词： carbon dots,fluorescent nanoprobe,(2,3-f)-pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid,Cu2+,S2?
更新于2025-09-23 15:23:52
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Highly Selective Fluorescent Probe Based on 2-(2′-Dansylamidophenyl)-Thiazole for Sequential Sensing of Copper(II) and Iodide Ions
摘要： A novel highly selective fluorescent probe based on 2-(20-dansylamidophenyl)-4-phenylthiazole (1) is developed for sequential sensing of copper(II) and iodide ions in acetonitrile. The fluorescence mechanism is based on cation-induced inhibition of excited-state intramolecular hydrogen transfer, intramolecular charge transfer, and metal–ligand electron/charge transfer. Subsequent iodide-induced extrusion of copper(II) results in partial revival of fluorescence. Probe 1 and its ensemble with copper (II) show high selectivity for copper(II) and iodide ions, respectively, in acetonitrile solution.
关键词： Excited-state intramolecular hydrogen transfer,I?,Cu2+,Intramolecular charge transfer,Dansyl-thiazole conjugate
更新于2025-09-23 15:23:52
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Ratiometric fluorescent probe based on pyrrole-modified rhodamine 6G hydrazone for the imaging of Cu2+ in lysosomes
摘要： A novel rhodamine-based Schiff base derivative was obtained via the simple condensation of substituted formyl-1H-pyrrole and rhodamine 6G hydrazone. Fluorescence resonance energy transfer enabled the subsequent use of the derivative as a naked-eye colorimetric and ratiometric fluorescent sensor for Cu2+ in semi-aqueous solution, and the existence of the morpholine group enabled the further application of the sensor in imaging Cu2+ in the lysosomes of HeLa cells.
关键词： ratiometric fluorescent probe,lysosome-targeting,rhodamine hydrazone,Cu2+,FRET,pyrrole
更新于2025-09-23 15:23:52
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Voids in Kesterites and the Influence of Lamellae Preparation by Focused Ion Beam for Transmission Electron Microscopy Analyses
摘要： Kesterite solar cells based on Cu2 ZnSnS4 and Cu2 ZnSnSe4 (CZTSe) are potential future candidates to be used in thin-film solar cells. The technology still has to be developed to a great extent and for this to happen, high levels of confidence in the characterization methods are required, so that improvements can be made on solid interpretations. In this study, we show that the interpretations of one of the most used characterization techniques in kesterites, scanning transmission electron microscopy (STEM), might be affected by its specimen preparation when using focused ion beam (FIB). Using complementary measurements based on scanning electron microscopy and Raman scattering spectroscopy, compelling evidence shows that secondary phases of ZnSe mixed in the bulk of CZTSe are the likely cause of the appearance of voids in STEM lamellae. Sputtering simulations support this interpretation by showing that Zn in a ZnSe matrix is preferentially sputtered compared with any metal atom in a CZTSe matrix.
关键词： Cu2 ZnSn(S, Se)4 (CZTSSe),thin-film solar cells,transmission electron microscopy (TEM),focused ion beam (FIB),kesterite
更新于2025-09-23 15:22:29
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Photocatalytic Self-Cleaning Cotton Fabrics Coated by Cu2(OH)PO4 under VIS/NIR Irradiation
摘要： In the present work, a mild strategy was employed to obtain cotton fabrics (CFs) coated with Cu2(OH)PO4 (CHP) nanoparticles to achieve self-cleaning property. The phytic acid (IP6) assisted method was employed to synthesize nanoparticles (CHP-IP6). The as-prepared coated cotton fabrics were characterized using the following techniques: Fourier-transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray (EDX) analysis, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and thermogravimetric analysis (TGA). The CHP-IP6 coated cotton fabrics showed significant photocatalytic activity, excellent photocatalytic stability, and good discoloration of methylene blue (MB) stains when exposed to sunlight, which could have important applications as tablecloths, household apparels, and industrial workwear.
关键词： photocatalysis,self-cleaning property,Cu2(OH)PO4,cotton fabric
更新于2025-09-23 15:22:29
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Extracellular biosynthesis of Cu2-xSe nanocrystallites with photocatalytic activity
摘要： Semiconductor Cu2-xSe nanospheres were successfully biosynthesized based on bioreduction of SeO3 2? into Se2? by the selenite-reducing bacterium, Pantoea agglomerans. The Cu2-xSe nanospheres had excellent crystallinity with a face-centered cubic structure and an average diameter of 80 nm. Composition and oxidation states analysis using X-ray photoelectron spectroscopy and X-ray energy dispersive spectroscopy followed by optical characterization using ultraviolet-visible and Fourier transform infra-red spectroscopy confirmed that the biosynthesized Cu2-xSe nanospheres were capped by proteins. The extracellular proteins which mediated biosynthesis were visualized by excitation–emission matrix fluorescence spectroscopy. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis revealed that the molecular masses of proteins were about 110, 50, 38, 35 and 25 kDa. The biosynthesized Cu2?xSe nanospheres showed an excellent and stable photocatalytical activity under sunlight irradiation in the degradation of methylene blue for four cycles. This study put forward a green and toilless way to manufacture copper selenide nanoparticles using a biological process.
关键词： Biosynthesis,Photocatalytical activity,Cu2-xSe,Extracellular proteins
更新于2025-09-23 15:21:21
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Noninvasive In Situ Ratiometric Imaging of Biometals Based on Self-Assembled Peptide Nanoribbon
摘要： Development of probes for accurate sensing and imaging of biometals in situ is still a growing interest owing to their crucial roles in cellular metabolism, neurotransmission, and apoptosis. Among them, Zn2+ and Cu2+ are two important cooperative biometals closely related to Alzheimer’s disease (AD). Herein, we developed a multifunctional probe based on self-assembling peptide nanoribbon for ratiometric sensing of Zn2+, Cu2+, or Zn2+ and Cu2+ simultaneously. Uniform peptide nanoribbon (AQZ@NR) was rationally designed by coassembling a Zn2+-specific ligand AQZ-modified peptide (AQZKL-7) with peptide KL-7. The nanoribbon further combined with Cu2+-sensitive near-infrared quantum dots (NIR QDs) and Alexa Fluor 633 as an inner reference molecule, which was endowed with the capability for ratiometric Zn2+ and Cu2+ imaging at the same time. The peptide-based probe exhibited good specificity to Zn2+ and Cu2+ without interference from other ions. Importantly, the nanoprobe was successfully applied for noninvasive Zn2+ and Cu2+ monitoring in both living cells and zebrafish via multicolor fluorescence imaging. This gives insights into the dynamic Zn2+ and Cu2+ distribution in an intracellular and in vivo mode, as well as understanding the neurotoxicity of high concentration of Zn2+ and Cu2+. Therefore, the self-assembled nanoprobe shows great promise in multiplexed detection of many other biometals and biomolecules, which will benefit the diagnosis and treatment of AD in clinical applications.
关键词： fluorescence imaging,biometals,ratiometric imaging,peptide nanoribbon,Cu2+,Zn2+,self-assembly,Alzheimer’s disease
更新于2025-09-23 15:21:01
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Dual-Emitting Quantum Dot Complex Nanoprobe for Ratiometric and Visual Detection of Hg2+ and Cu2+ ions
摘要： Herein we report the use of a dual emitting quantum dot complex (QDC; composed of blue emitting metal-methylsalicylaldimine complex being on the surface of orange emitting Mn2+-doped ZnS quantum dot) for ratiometric and visual detection of Hg2+ and Cu2+ ions - following the alterations in luminescence color, intensity ratio, chromaticity and hue of the QDC.
关键词： Hg2+ ions,Cu2+ ions,ratiometric detection,quantum dot complex,visual detection
更新于2025-09-23 15:21:01